MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 51–60 (out of 250)

Page: Previous 1 2 3 4 5 6 7 8 9 10 11 … 25 Next 

-51

Accession Score Description
1 SYA_PSEPG 110 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2
Score Mass Matches Sequences emPAI
51.1 SYA_PSEPG 110 95050 10 (4) 9 (4) 0.10
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2
2 samesets of SYA_PSEPG
SYA_PSEPK 110 95109 10 (4) 9 (4) 0.10
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1
SYA_PSEP1 110 95108 10 (4) 9 (4) 0.10
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2

-10 peptide matches (10 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
169   529.3482 528.3409 528.3384 4.85 0 14 0.9 +1Score > 41 indicates identity
Score > 26 indicates homology		 K.VLAAR.L
444   360.7380 719.4614 719.4581 4.61 0 17 0.51 +1Score > 29 indicates identity
Score > 26 indicates homology		 K.IIALYK.D
811   443.2950 884.5754 884.5695 6.70 0 8 0.31 +1Score > 32 indicates identity
Score > 16 indicates homology		 K.VVLVAGVTK.D
1049   493.7662 985.5178 985.5192 -1.41 1 31 0.41 +1Score > 41 indicates identity
Score > 40 indicates homology		 U K.QLEDIVNR.E
1260   542.3491 1082.6836 1082.6699 12.7 0 36 0.022 +1Score > 32 indicates identity U K.ALLALVDQLK.N
1393   572.8242 1143.6338 1143.6248 7.92 1 42 0.0076 +1Score > 41 indicates identity
Score > 34 indicates homology		 U K.IISEGGVASGVR.R
1440   389.2168 1164.6286 1164.6251 2.96 1 18 3 +3Score > 39 indicates identity
Score > 35 indicates homology		 R.QVLGEHVQQK.G
2355   794.8976 1587.7806 1587.7740 4.19 1 91 3.3e-008 +1Score > 41 indicates identity
Score > 28 indicates homology		 U K.AASAAGDDLSNAAVEVK.G
4245   713.8888 2851.5261 2851.4868 13.8 1 24 0.009 +1Score > 34 indicates identity
Score > 16 indicates homology		 U R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ)
4246   951.5192 2851.5358 2851.4868 17.2 1 31 0.099 +1Score > 33 indicates identity U R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ)

3 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
SYA_PSEE4 107 95309 51.1
Alanyl-tRNA synthetase OS=Pseudomonas entomophila (strain L48) GN=alaS PE=3 SV=1
SYA_PSEPF 45 94840 51.1
Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=alaS PE=3 SV=1
1 sameset of SYA_PSEPF
SYA_PSEPW 45 94991
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain W619) GN=alaS PE=3 SV=2
SYA_PSE14 36 94784 51.1
Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=alaS PE=3 SV=1
8 samesets of SYA_PSE14
SYA_PSEF5 36 95022
Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=alaS PE=3 SV=1
SYA_PSESM 36 94865
Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=alaS PE=3 SV=1
SYA_PSEU2 36 94751
Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=alaS PE=3 SV=2
SYA_PSEAB 36 95112
Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=alaS PE=3 SV=1
SYA_PSEAE 36 95099
Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=alaS PE=3 SV=1
SYA_PSEMY 36 94797
Alanyl-tRNA synthetase OS=Pseudomonas mendocina (strain ymp) GN=alaS PE=3 SV=2
SYA_PSEU5 36 95029
Alanyl-tRNA synthetase OS=Pseudomonas stutzeri (strain A1501) GN=alaS PE=3 SV=2
SYA_PSEA7 36 94997
Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain PA7) GN=alaS PE=3 SV=1

+52

Accession Score Description
1 RL4_PSE14 110 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+53

Accession Score Description
1 RL3_PSEE4 107 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+54

Accession Score Description
1 LEU1_PSEP1 103 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+55

Accession Score Description
1 CISY_PSEAE 103 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+56

Accession Score Description
1 IF2_PSEE4 102 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+57

Accession Score Description
1 SODF_PSEPK 99 Superoxide dismutase [Fe] OS=Pseudomonas putida (strain KT2440) GN=sodB PE=3 SV=3

+58

Accession Score Description
1 PORF_PSEAE 100 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+59

Accession Score Description
1 RP5M_PSEPK 99 Probable sigma(54) modulation protein OS=Pseudomonas putida (strain KT2440) GN=rpoX PE=3 SV=1

+60

Accession Score Description
1 CAPA_PSEFR 98 Cold shock protein capA (Fragment) OS=Pseudomonas fragi GN=capA PE=2 SV=1
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 … 25 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/