MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 250)

Page: 1 2 3 4 5 6 … 25 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1177 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_CARRP 73 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1045 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 EFTU_BACAA 271 Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
4 EFTU_ANATD 83 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 525 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
5 ATPG_RHOP5 40 description
3 ATPB_LEGPA 167 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
2 CH60_PSEPK 473 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
6 CH602_PROMS 39 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1
4 CH602_SINMW 59 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPG 522 ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 ATPA_RICAH 112 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1

+4

Accession Score Description
1 ARCA_PSEPK 463 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-5

Accession Score Description
1 OTCC_PSEPK 391 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
Score Mass Matches Sequences emPAI
5.1 OTCC_PSEPK 391 38115 34 (23) 15 (11) 1.71
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-34 peptide matches (24 non-duplicate, 10 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
154   499.3263 498.3190 498.3166 4.95 0 47 0.0032 +1Score > 34 indicates identity U R.IAAPK.A
382   345.6852 689.3558 689.3530 4.07 0 36 0.13 +1Score > 42 indicates identity
Score > 40 indicates homology		 K.LGMDVR.I
779   436.2322 870.4498 870.4460 4.40 0 36 0.1 +1Score > 39 indicates identity
Score > 39 indicates homology		 M.AFNIHNR.N
801   879.4998 878.4925 878.4861 7.26 0 36 0.069 +1Score > 37 indicates identity R.YLLDLSR.D
888   916.5001 915.4928 915.4913 1.66 1 57 0.0021 +1Score > 43 indicates identity U R.ITLTEDPK.A
889   458.7543 915.4940 915.4913 3.00 1 27 0.45 +1Score > 43 indicates identity
Score > 36 indicates homology		 U R.ITLTEDPK.A
1136 +3 1015.6130 1014.6057 1014.5961 9.49 0 16 0.38 +1Score > 37 indicates identity
Score > 25 indicates homology		 U K.AILVSTLADL.-
1226   530.7451 1059.4756 1059.4695 5.80 1 40 0.041 +1Score > 39 indicates identity U R.MYDAIEYR.G
1322   1118.6259 1117.6186 1117.6131 4.92 1 70 5e-005 +1Score > 40 indicates identity U K.GNNIALIFEK.T
1323 +2 559.8181 1117.6216 1117.6131 7.62 1 55 0.00073 +1Score > 39 indicates identity
Score > 36 indicates homology		 U K.GNNIALIFEK.T
1521   402.2043 1203.5911 1203.5884 2.23 1 26 0.051 +1Score > 42 indicates identity
Score > 25 indicates homology		 U K.YTGTEQQHLK.G
1522   602.8039 1203.5932 1203.5884 4.03 1 51 0.00022 +1Score > 42 indicates identity
Score > 27 indicates homology		 U K.YTGTEQQHLK.G
1772   330.9320 1319.6989 1319.6946 3.27 1 45 0.0006 +1Score > 40 indicates identity
Score > 25 indicates homology		 U R.NLLSLEHHTTR.E
1774   660.8571 1319.6996 1319.6946 3.84 1 76 1.4e-005 +1Score > 40 indicates identity U R.NLLSLEHHTTR.E
1776 +2 440.9074 1319.7004 1319.6946 4.39 1 47 0.00055 +1Score > 40 indicates identity
Score > 27 indicates homology		 U R.NLLSLEHHTTR.E
1778   441.2408 1320.7006 1320.6786 16.6 1 11 0.62 +2Score > 40 indicates identity
Score > 21 indicates homology		 U R.NLLSLEHHTTR.E + Deamidated (NQ)
1829 +1 672.8730 1343.7314 1343.7231 6.23 0 51 0.00028 +1Score > 40 indicates identity
Score > 28 indicates homology		 U R.NNMGNSLLLIGAK.L
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.raw

Score > 41 indicates identity

Score > 24 indicates homology
1834 +2 450.9003 1349.6791 1349.6728 4.65 1 40 0.0013 -1Score > 41 indicates identity
Score > 24 indicates homology		 U K.ALWPHDDLVER.C
-2.66 1 9 1.8 2 QPLKHNVIVGNE   + 3 Deamidated (NQ)
-0.70 0 7 2.4 3 GTATHPGNNVGIGR  
-14.0 0 7 2.5 4 GPQSPFQHILPK   + 2 Deamidated (NQ)
-2.69 0 7 2.9 5 SYTVTNGADVVPK  
16.0 1 6 3 6 GVQYNRDIINR   + 3 Deamidated (NQ)
-16.5 1 6 3.3 7 IACIFSIQLER   + Deamidated (NQ)
-2.69 0 6 3.5 8 VGSYVVNGSGSPVK   + Deamidated (NQ)
-11.0 1 5 4.1 9 FGIVTSGNALSER  
-2.67 1 5 4.1 10 TGNETLQFIAVR   + 2 Deamidated (NQ)
1837   675.8485 1349.6824 1349.6728 7.16 1 31 0.015 +1Score > 41 indicates identity
Score > 25 indicates homology		 U K.ALWPHDDLVER.C
2275   521.2874 1560.8404 1560.8334 4.49 1 35 0.0016 +1Score > 39 indicates identity
Score > 19 indicates homology		 U K.QLKPYQVNAELMK.S
2276   781.4292 1560.8438 1560.8334 6.72 1 49 0.00028 +1Score > 39 indicates identity
Score > 26 indicates homology		 U K.QLKPYQVNAELMK.S
3415   547.5189 2186.0465 2186.0392 3.33 1 40 0.0007 +1Score > 39 indicates identity
Score > 20 indicates homology		 U R.EHSDKPLHDISYAYLGDAR.N
4386   756.3879 3021.5225 3021.4729 16.4 1 8 1 +1Score > 36 indicates identity
Score > 20 indicates homology		 U K.FAGVPVFNGLTDEYHPTQMIADVLTMR.E
4389   1008.5105 3022.5097 3022.4569 17.5 1 62 2.9e-006 +1Score > 36 indicates identity
Score > 19 indicates homology		 U K.FAGVPVFNGLTDEYHPTQMIADVLTMR.E + Deamidated (NQ)

7 subsets and intersections (43 subset proteins in total)

Score Mass Subset of
OTCC_PSEAE 177 38255 5.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas aeruginosa GN=arcB PE=1 SV=3
OTCC_PSEME 117 38189 5.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas mendocina GN=arcB PE=3 SV=3
OTCC_BURMA 66 38278 5.1
Ornithine carbamoyltransferase, catabolic OS=Burkholderia mallei GN=arcB PE=3 SV=1
3 samesets of OTCC_BURMA
OTCC_BURPS 66 38278
Ornithine carbamoyltransferase, catabolic OS=Burkholderia pseudomallei GN=arcB PE=3 SV=1
OTCC_HAEGA 66 37705
Ornithine carbamoyltransferase, catabolic OS=Haemophilus gallinarum GN=arcB PE=3 SV=1
OTC_HAEDU 66 37722
Ornithine carbamoyltransferase OS=Haemophilus ducreyi GN=argF PE=3 SV=1
OTCC_HAEIN 61 37908 5.1
Ornithine carbamoyltransferase, catabolic OS=Haemophilus influenzae GN=arcB PE=3 SV=1
2 samesets of OTCC_HAEIN
OTC_HAEIG 61 37881
Ornithine carbamoyltransferase OS=Haemophilus influenzae (strain PittGG) GN=arcB PE=3 SV=1
OTC_MANSM 61 37793
Ornithine carbamoyltransferase OS=Mannheimia succiniciproducens (strain MBEL55E) GN=argF PE=3 SV=1
OTCC_CHRVO 56 38025 5.1
Ornithine carbamoyltransferase, catabolic OS=Chromobacterium violaceum GN=arcB PE=3 SV=1
AMN1_PICAN 47 0 5.1
description
19 samesets of AMN1_PICAN
DNAE2_RALSO 47 120147
Error-prone DNA polymerase OS=Ralstonia solanacearum GN=dnaE2 PE=3 SV=1
OTC_CLOAB 47 0
description
SYA_IGNH4 47 0
description
CIPKB_ORYSJ 47 0
description
FAS_RAT 47 0
description
DOP1_EMENI 47 0
description
IML1_NEUCR 47 0
description
CHDM_DROME 47 0
description
GB_MCMVS 47 0
description
MURI_LACAC 47 0
description
THEG_HUMAN 47 0
description
BOP1_DICDI 47 0
description
DNAE2_RALPJ 47 0
description
IMPA3_MOUSE 47 0
description
RL3_SPHWW 47 0
description
YHC3_SCHPO 47 0
description
RS4_DROME 47 0
description
RL32_CARHZ 47 0
description
UNC53_CAEEL 47 0
description
OTCC_RHIET 40 38135 5.1
Ornithine carbamoyltransferase, catabolic OS=Rhizobium etli GN=arcB PE=3 SV=1
12 samesets of OTCC_RHIET
OTC_ACTPJ 40 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=arcB PE=3 SV=1
OTC_NEIGO 40 36823
Ornithine carbamoyltransferase OS=Neisseria gonorrhoeae GN=argF PE=3 SV=1
OTC_NEILA 40 28761
Ornithine carbamoyltransferase (Fragment) OS=Neisseria lactamica GN=argF PE=3 SV=1
OTC_NEIMA 40 36795
Ornithine carbamoyltransferase OS=Neisseria meningitidis serogroup A GN=argF PE=3 SV=1
OTC_NEIMB 40 36807
Ornithine carbamoyltransferase OS=Neisseria meningitidis serogroup B GN=argF PE=3 SV=1
OTC_NEIME 40 28974
Ornithine carbamoyltransferase (Fragment) OS=Neisseria meningitidis GN=argF PE=3 SV=2
OTC_NEIPE 40 25521
Ornithine carbamoyltransferase (Fragment) OS=Neisseria perflava GN=argF PE=3 SV=1
OTC_NEIPO 40 28876
Ornithine carbamoyltransferase (Fragment) OS=Neisseria polysaccharea GN=argF PE=3 SV=1
OTC_NEIG1 40 36768
Ornithine carbamoyltransferase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=argF PE=3 SV=1
OTC_ACTP2 40 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=argF PE=3 SV=1
OTC_ACTP7 40 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=arcB PE=3 SV=1
OTCC_RHIME 40 37978
Ornithine carbamoyltransferase, catabolic OS=Rhizobium meliloti GN=arcB PE=3 SV=1

+6

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 350 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
2 RPOC_PSEA7 244 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 FTSZ_PSEPK 95 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+7

Accession Score Description
1 DLDH2_PSEPU 349 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+8

Accession Score Description
1 ADHP_ECOLI 327 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+9

Accession Score Description
1 ACON2_PSEAE 321 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+10

Accession Score Description
1 SUCC_PSEPK 313 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
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