MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 250)

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+41

Accession Score Description
1 ILVC_PSEPG 120 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+42

Accession Score Description
1 AMPA_PSEPG 120 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+43

Accession Score Description
1 YJJK_ECOLI 119 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+44

Accession Score Description
1 ARGD_PSEPK 119 Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1

+45

Accession Score Description
1 YEAG_ECOLI 119 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+46

Accession Score Description
1 RPOA_PSEPK 118 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+47

Accession Score Description
1 STHA_PSEPG 117 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

-48

Accession Score Description
Family member distances as a dendrogram 1 GLYA2_PSEF5 116 Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1
2 GLYA1_PSEF5 39 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
GLYA2_PSEF5 116 45064 5 (4) 5 (4) 0.22
Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1
2 samesets of GLYA2_PSEF5
GLYA1_PSEPK 116 44813 4 (4) 4 (4) 0.22
Serine hydroxymethyltransferase 1 OS=Pseudomonas putida (strain KT2440) GN=glyA1 PE=3 SV=1
GLYA3_PSEPF 116 44970 4 (4) 4 (4) 0.22
Serine hydroxymethyltransferase 3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=glyA3 PE=3 SV=1
GLYA1_PSEF5 39 45149 4 (2) 4 (2) 0.10
Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
173   531.2930 530.2857 530.2812 8.47 0 7 1.1 +5Score > 46 indicates identity
Score > 20 indicates homology		 X E.ANVAR.Q + Deamidated (NQ)
577   391.7312 781.4478 781.4446 4.12 0 26 0.057 +1Score > 35 indicates identity
Score > 26 indicates homology		 U X R.AHITVNK.N
885   458.2690 914.5234 914.5185 5.37 0 44 0.03 +1Score > 41 indicates identity U X R.IGTPAVTTR.G
979   475.2608 948.5070 948.5029 4.37 0 33 0.34 +1Score > 41 indicates identity U X R.FGTPAVTTR.G
1017   482.2694 962.5242 962.5185 5.93 0 31 0.025 +1Score > 41 indicates identity
Score > 27 indicates homology		 U X R.SPFVTSGLR.F
2770   900.4642 1798.9138 1798.8850 16.0 0 72 8.4e-007 +1Score > 40 indicates identity
Score > 24 indicates homology		 U X K.NAVPNDPQSPFVTSGLR.I + Deamidated (NQ)
3205   693.0538 2076.1396 2076.1004 18.9 1 21 1 +1Score > 35 indicates identity
Score > 34 indicates homology		 U X K.LNSAVFPGAQGGPLEHVIAAK.A + Deamidated (NQ)
3331   716.0708 2145.1906 2145.1834 3.35 1 30 0.002 +1Score > 33 indicates identity
Score > 16 indicates homology		 U X R.GFDVVSGGTKNHLFLLSLIK.Q + Deamidated (NQ)
3365   721.7217 2162.1433 2162.1008 19.6 0 56 1e-005 +1Score > 37 indicates identity
Score > 18 indicates homology		 U X R.GYDVVSGGTDNHLFLVSLIR.Q + Deamidated (NQ)

+12 subsets and intersections (264 subset proteins in total)

+49

Accession Score Description
1 RRF_ACTSZ 116 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+50

Accession Score Description
1 RL25_PSEPK 114 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2
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