MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 120 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPG | 120 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJJK_ECOLI | 119 | Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARGD_PSEPK | 119 | Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 119 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 118 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPG | 117 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 47.1 | STHA_PSEPG |
117 | 51202 | 6 (3) | 6 (3) | 0.14 | |
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1 | |||||||
| 2 samesets of STHA_PSEPG | |||||||
| STHA_PSEPK |
117 | 51159 | 6 (3) | 6 (3) | 0.14 | ||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3 | |||||||
| STHA_PSEP1 |
117 | 51159 | 6 (3) | 6 (3) | 0.14 | ||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sthA PE=3 SV=1 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1373 |
569.8068 | 1137.5990 | 1137.5859 | 11.6 | 0 | 5 | 1.5 | 4Score > 40 indicates identity Score > 20 indicates homology |
R.WFSFPDVLK.S | ||
| 1953 |
469.6080 | 1405.8022 | 1405.7929 | 6.59 | 1 | 40 | 0.006 | 1Score > 35 indicates identity Score > 31 indicates homology |
U | R.VEGLDNGVILHLK.S | |
| 2584 |
568.6260 | 1702.8562 | 1702.8526 | 2.09 | 0 | 43 | 0.0007 | 1Score > 40 indicates identity Score > 24 indicates homology |
U | R.VYDSDTILSLSHTPR.K | |
| 3341 |
1076.0771 | 2150.1396 | 2150.1147 | 11.6 | 1 | 28 | 0.47 | 1Score > 37 indicates identity |
R.FVNDVPTGIYTIPEISSIGK.N + Deamidated (NQ) | ||
| 4112 |
918.7986 | 2753.3740 | 2753.3409 | 12.0 | 0 | 86 | 2.6e-008 | 1Score > 37 indicates identity Score > 23 indicates homology |
U | R.TTVPNIYGAGDVIGWPSLASAAHDQGR.S + Deamidated (NQ) | |
| 4410 |
1018.5035 | 3052.4887 | 3052.5254 | -12.0 | 1 | 10 | 0.29 | 1Score > 37 indicates identity Score > 17 indicates homology |
R.GQLLSFLDSEISQALSYHFSNNNITVR.H |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| STHA_PSEA7 |
56 | 51343 | 47.1 | ||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain PA7) GN=sthA PE=3 SV=1 | |||||
| 4 samesets of STHA_PSEA7 | |||||
| STHA_PSEPW |
56 | 51152 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain W619) GN=sthA PE=3 SV=1 | |||||
| STHA_PSEAB |
56 | 51357 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sthA PE=3 SV=1 | |||||
| STHA_PSEAE |
56 | 51357 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa GN=sthA PE=1 SV=2 | |||||
| STHA_PSEA8 |
56 | 51357 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain LESB58) GN=sthA PE=3 SV=1 | |||||
| STHA_PSEE4 |
43 | 51180 | 47.1 | ||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas entomophila (strain L48) GN=sthA PE=3 SV=1 | |||||
| STHA_AZOVD |
40 | 51527 | 47.1 | ||
| Soluble pyridine nucleotide transhydrogenase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=sthA PE=3 SV=1 | |||||
| 5 samesets of STHA_AZOVD | |||||
| STHA_PSEFL |
40 | 51318 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens GN=sthA PE=1 SV=3 | |||||
| STHA_PSEFS |
40 | 51305 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens (strain SBW25) GN=sthA PE=3 SV=1 | |||||
| STHA_PSEU2 |
40 | 51036 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sthA PE=3 SV=2 | |||||
| STHA_PSE14 |
40 | 51049 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sthA PE=3 SV=1 | |||||
| STHA_AZOVI |
40 | 51527 | |||
| Soluble pyridine nucleotide transhydrogenase OS=Azotobacter vinelandii GN=sthA PE=1 SV=1 | |||||
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLYA2_PSEF5 | 116 | Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1 | |
| 2 | GLYA1_PSEF5 | 39 | Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1 | |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RRF_ACTSZ | 116 | Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 114 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
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