MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 120 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPG | 120 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJJK_ECOLI | 119 | Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARGD_PSEPK | 119 | Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 119 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 118 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 46.1 | RPOA_PSEPK |
118 | 36721 | 7 (4) | 6 (3) | 0.28 | |
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 | |||||||
| 2 samesets of RPOA_PSEPK | |||||||
| RPOA_PSEP1 |
118 | 36721 | 7 (4) | 6 (3) | 0.28 | ||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoA PE=3 SV=1 | |||||||
| RPOA_PSEE4 |
118 | 36721 | 7 (4) | 6 (3) | 0.28 | ||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas entomophila (strain L48) GN=rpoA PE=3 SV=1 | |||||||
7 peptide matches (7 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1024 |
485.7845 | 969.5544 | 969.5495 | 5.14 | 1 | 34 | 0.082 | 1Score > 39 indicates identity Score > 36 indicates homology |
K.ITLEPLER.G | ||
| 1181 |
517.7873 | 1033.5600 | 1033.5556 | 4.29 | 1 | 34 | 0.06 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | R.IAYVVENAR.V | |
| 1386 |
381.5420 | 1141.6042 | 1141.5992 | 4.32 | 0 | 18 | 0.074 | 1Score > 40 indicates identity Score > 19 indicates homology |
R.GFGHTLGNALR.R | ||
| 1594 |
616.8409 | 1231.6672 | 1231.6561 | 9.07 | 0 | 63 | 9.1e-005 | 1Score > 40 indicates identity Score > 35 indicates homology |
U | R.LQLDASFSPVR.R | |
| 1633 |
625.8489 | 1249.6832 | 1249.6779 | 4.28 | 0 | 62 | 5.7e-005 | 1Score > 39 indicates identity Score > 33 indicates homology |
U | R.HIDVQVVSPTR.A | |
| 1634 |
417.5687 | 1249.6843 | 1249.6779 | 5.10 | 0 | 36 | 0.007 | 1Score > 39 indicates identity Score > 27 indicates homology |
U | R.HIDVQVVSPTR.A | |
| 2288 |
784.4241 | 1566.8336 | 1566.8042 | 18.8 | 1 | 43 | 0.00025 | 1Score > 39 indicates identity Score > 20 indicates homology |
U | K.AENIYYIGDLIQR.T |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RPOA_PSE14 |
88 | 36765 | 46.1 | ||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoA PE=3 SV=1 | |||||
| 6 samesets of RPOA_PSE14 | |||||
| RPOA_PSEPF |
88 | 36723 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSEPU |
88 | 36695 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSESM |
88 | 36765 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. tomato GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSEU2 |
88 | 36765 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSEF5 |
88 | 36721 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSEU5 |
88 | 36620 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas stutzeri (strain A1501) GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSEA7 |
79 | 36741 | 46.1 | ||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain PA7) GN=rpoA PE=3 SV=1 | |||||
| 4 samesets of RPOA_PSEA7 | |||||
| RPOA_PSEAB |
79 | 36741 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoA PE=3 SV=1 | |||||
| RPOA_PSEAE |
79 | 36741 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa GN=rpoA PE=3 SV=2 | |||||
| RPOA_PSEMY |
79 | 36661 | |||
| DNA-directed RNA polymerase subunit alpha OS=Pseudomonas mendocina (strain ymp) GN=rpoA PE=3 SV=1 | |||||
| RPOA_SACD2 |
79 | 36391 | |||
| DNA-directed RNA polymerase subunit alpha OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoA PE=3 SV=1 | |||||
| RPOA_ACIAC |
43 | 36282 | 46.1 | ||
| DNA-directed RNA polymerase subunit alpha OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rpoA PE=3 SV=1 | |||||
| 58 samesets of RPOA_ACIAC |
|||||
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPG | 117 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLYA2_PSEF5 | 116 | Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1 | |
| 2 | GLYA1_PSEF5 | 39 | Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1 | |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RRF_ACTSZ | 116 | Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 114 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
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