MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 120 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPG | 120 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJJK_ECOLI | 119 | Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARGD_PSEPK | 119 | Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 119 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 45.1 | YEAG_ECOLI |
119 | 74776 | 6 (4) | 6 (4) | 0.13 | |
| Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 | |||||||
| 2 samesets of YEAG_ECOLI | |||||||
| YEAG_ECOL6 |
119 | 74776 | 6 (4) | 6 (4) | 0.13 | ||
| Uncharacterized protein yeaG OS=Escherichia coli O6 GN=yeaG PE=3 SV=1 | |||||||
| YEAG_ECO57 |
119 | 74776 | 6 (4) | 6 (4) | 0.13 | ||
| Uncharacterized protein yeaG OS=Escherichia coli O157:H7 GN=yeaG PE=3 SV=1 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1043 |
489.7373 | 977.4600 | 977.4566 | 3.49 | 1 | 33 | 0.2 | 1Score > 42 indicates identity Score > 39 indicates homology |
U | K.NNEAFLDR.V | |
| 1300 |
552.3195 | 1102.6244 | 1102.6135 | 9.95 | 1 | 48 | 0.001 | 1Score > 38 indicates identity Score > 30 indicates homology |
U | R.NEIVNFVLR.A | |
| 1409 |
385.2253 | 1152.6541 | 1152.6502 | 3.33 | 1 | 28 | 0.084 | 1Score > 37 indicates identity Score > 30 indicates homology |
U | K.IEKPAGISNPK.D | |
| 1764 |
657.8984 | 1313.7822 | 1313.7707 | 8.77 | 0 | 26 | 0.0083 | 1Score > 34 indicates identity Score > 18 indicates homology |
U | K.QILYLLGPVGGGK.S | |
| 2022 |
481.6325 | 1441.8757 | 1441.8657 | 6.93 | 1 | 31 | 0.0068 | 1Score > 29 indicates identity Score > 22 indicates homology |
U | K.KQILYLLGPVGGGK.S | |
| 2755 |
893.9279 | 1785.8412 | 1785.8381 | 1.78 | 1 | 81 | 3.9e-008 | 1Score > 40 indicates identity Score > 19 indicates homology |
U | K.TEPGDENNQDISALVGK.V |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 118 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPG | 117 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLYA2_PSEF5 | 116 | Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1 | |
| 2 | GLYA1_PSEF5 | 39 | Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1 | |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RRF_ACTSZ | 116 | Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 114 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
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