MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 250)

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+41

Accession Score Description
1 ILVC_PSEPG 120 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

-42

Accession Score Description
1 AMPA_PSEPG 120 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1
Score Mass Matches Sequences emPAI
42.1 AMPA_PSEPG 120 52694 9 (4) 7 (4) 0.19
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1
1 sameset of AMPA_PSEPG
AMPA_PSEPK 120 52857 9 (4) 7 (4) 0.18
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

-9 peptide matches (8 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
470   732.4291 731.4218 731.4177 5.58 0 66 0.00015 +1Score > 40 indicates identity U K.SVAAASVK.T
471   366.7182 731.4218 731.4177 5.62 0 29 0.1 +1Score > 40 indicates identity
Score > 32 indicates homology		 U K.SVAAASVK.T
558   386.7516 771.4886 771.4854 4.15 0 28 0.45 +1Score > 37 indicates identity U R.VLLVGSGK.E
1301 +1 552.8273 1103.6400 1103.6339 5.59 0 45 0.0049 +1Score > 37 indicates identity
Score > 34 indicates homology		 U R.LIVLNYQGGK.K
1516   401.2501 1200.7285 1200.7230 4.53 1 31 0.069 +1Score > 32 indicates identity U K.KADKPFVLVGK.G
1671   635.3652 1268.7158 1268.7088 5.52 1 43 0.014 +1Score > 38 indicates identity
Score > 37 indicates homology		 U K.TATLVIPVGENR.K
2907   628.6738 1882.9996 1882.9748 13.1 0 71 4.2e-006 +1Score > 38 indicates identity
Score > 30 indicates homology		 U K.GLNGADAVLALDDIAVSNR.D
3045   991.4655 1980.9164 1980.9000 8.31 0 2 1.4 +7Score > 39 indicates identity
Score > 16 indicates homology		 U K.DLGMGAFYAVGQGSDQPPR.L + Oxidation (M)

4 subsets and intersections (4 subset proteins in total)

Score Mass Subset of
AMPA_PSEPU 80 52835 42.1
Cytosol aminopeptidase OS=Pseudomonas putida GN=pepA PE=3 SV=1
AMPA_PSEP1 71 52796 42.1
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pepA PE=3 SV=1
AMPA_PSEPW 66 52844 42.1
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain W619) GN=pepA PE=3 SV=1
AMPA_PSEE4 45 52459 42.1
Probable cytosol aminopeptidase OS=Pseudomonas entomophila (strain L48) GN=pepA PE=3 SV=1

+43

Accession Score Description
1 YJJK_ECOLI 119 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+44

Accession Score Description
1 ARGD_PSEPK 119 Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1

+45

Accession Score Description
1 YEAG_ECOLI 119 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+46

Accession Score Description
1 RPOA_PSEPK 118 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+47

Accession Score Description
1 STHA_PSEPG 117 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+48

Accession Score Description
Family member distances as a dendrogram 1 GLYA2_PSEF5 116 Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1
2 GLYA1_PSEF5 39 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

+49

Accession Score Description
1 RRF_ACTSZ 116 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+50

Accession Score Description
1 RL25_PSEPK 114 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2
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