MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 250)

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-41

Accession Score Description
1 ILVC_PSEPG 120 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1
Score Mass Matches Sequences emPAI
41.1 ILVC_PSEPG 120 36575 5 (5) 4 (4) 0.36
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1
3 samesets of ILVC_PSEPG
ILVC_PSEPK 120 36575 5 (5) 4 (4) 0.36
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain KT2440) GN=ilvC PE=3 SV=1
ILVC_PSEPW 120 36561 5 (5) 4 (4) 0.36
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain W619) GN=ilvC PE=3 SV=1
ILVC_PSEP1 120 36575 5 (5) 4 (4) 0.36
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ilvC PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1493   593.3376 1184.6606 1184.6475 11.1 0 54 0.00066 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.ADLDVIMIAPK.A
1749   654.3405 1306.6664 1306.6630 2.67 0 69 2.3e-005 +1Score > 41 indicates identity
Score > 35 indicates homology		 U K.NVALSYASGVGGGR.T
2872   930.4846 1858.9546 1858.9425 6.55 0 61 3.4e-005 +1Score > 40 indicates identity
Score > 29 indicates homology		 U K.GGGIPDLIAIYQDASGNAK.N
2873   620.6591 1858.9555 1858.9425 6.99 0 26 0.024 +1Score > 39 indicates identity
Score > 22 indicates homology		 U K.GGGIPDLIAIYQDASGNAK.N
3446   551.2908 2201.1341 2201.1018 14.7 0 18 0.024 +1Score > 38 indicates identity
Score > 15 indicates homology		 U K.GATLAFSHGFSIHYNQVVPR.A + Deamidated (NQ)

5 subsets and intersections (55 subset proteins in total)

Score Mass Subset of
ILVC_PSEE4 120 36674 41.1
Ketol-acid reductoisomerase OS=Pseudomonas entomophila (strain L48) GN=ilvC PE=3 SV=1
ILVC_PSE14 88 36531 41.1
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=ilvC PE=3 SV=1
3 samesets of ILVC_PSE14
ILVC_PSEMY 88 36501
Ketol-acid reductoisomerase OS=Pseudomonas mendocina (strain ymp) GN=ilvC PE=3 SV=1
ILVC_PSESM 88 36589
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. tomato GN=ilvC PE=3 SV=1
ILVC_PSEU2 88 36531
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ilvC PE=3 SV=1
ILVC_PSEA7 85 36729 41.1
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain PA7) GN=ilvC PE=3 SV=1
6 samesets of ILVC_PSEA7
ILVC_PSEAB 85 36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=ilvC PE=3 SV=1
ILVC_PSEAE 85 36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa GN=ilvC PE=1 SV=1
ILVC_PSEFS 85 36416
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvC PE=3 SV=1
ILVC_PSEPF 85 36441
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ilvC PE=3 SV=1
ILVC_PSEF5 85 36441
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=ilvC PE=3 SV=1
ILVC_PSEA8 85 36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain LESB58) GN=ilvC PE=3 SV=1
ILVC_ACIAD 69 37091 41.1
Ketol-acid reductoisomerase OS=Acinetobacter sp. (strain ADP1) GN=ilvC PE=3 SV=1
6 samesets of ILVC_ACIAD
ILVC_ACIBC 69 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain ACICU) GN=ilvC PE=3 SV=1
ILVC_ACIBS 69 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain SDF) GN=ilvC PE=3 SV=1
ILVC_ACIBT 69 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=ilvC PE=3 SV=2
ILVC_ACIBY 69 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AYE) GN=ilvC PE=3 SV=1
ILVC_ACIB3 69 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AB307-0294) GN=ilvC PE=3 SV=1
ILVC_ACIB5 69 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AB0057) GN=ilvC PE=3 SV=1
ILVC_ALCBS 54 36559 41.1
Ketol-acid reductoisomerase OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=ilvC PE=3 SV=2
+35 samesets of ILVC_ALCBS

+42

Accession Score Description
1 AMPA_PSEPG 120 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+43

Accession Score Description
1 YJJK_ECOLI 119 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+44

Accession Score Description
1 ARGD_PSEPK 119 Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1

+45

Accession Score Description
1 YEAG_ECOLI 119 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+46

Accession Score Description
1 RPOA_PSEPK 118 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+47

Accession Score Description
1 STHA_PSEPG 117 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+48

Accession Score Description
Family member distances as a dendrogram 1 GLYA2_PSEF5 116 Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1
2 GLYA1_PSEF5 39 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

+49

Accession Score Description
1 RRF_ACTSZ 116 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+50

Accession Score Description
1 RL25_PSEPK 114 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2
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