MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 250)

Page: 1 2 3 4 5 6 … 25 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1177 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_CARRP 73 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1045 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 EFTU_BACAA 271 Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
4 EFTU_ANATD 83 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 525 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
5 ATPG_RHOP5 40 description
3 ATPB_LEGPA 167 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
2 CH60_PSEPK 473 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
6 CH602_PROMS 39 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1
4 CH602_SINMW 59 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPG 522 ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 ATPA_RICAH 112 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1

-4

Accession Score Description
1 ARCA_PSEPK 463 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1
Score Mass Matches Sequences emPAI
4.1 ARCA_PSEPK 463 46775 32 (17) 14 (9) 0.86
Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-32 peptide matches (25 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
250   621.2919 620.2846 620.2806 6.52 0 16 0.093 +1Score > 42 indicates identity
Score > 18 indicates homology		 U R.DPINY.-
408   702.3939 701.3866 701.3860 0.82 0 19 1.2 +3Score > 42 indicates identity
Score > 32 indicates homology		 K.WILDR.K
409   351.7015 701.3884 701.3860 3.42 0 16 1.3 +2Score > 42 indicates identity
Score > 30 indicates homology		 K.WILDR.K
916   925.5746 924.5673 924.5644 3.15 1 50 0.0013 +1Score > 34 indicates identity U K.EVIVAGLPK.S
917 +1 463.2919 924.5692 924.5644 5.24 1 33 0.06 +1Score > 34 indicates identity U K.EVIVAGLPK.S
969   316.4920 946.4542 946.4508 3.52 1 14 0.61 +2Score > 42 indicates identity
Score > 24 indicates homology		 U K.YGVHSEAGK.L
1106   504.7422 1007.4698 1007.4713 -1.41 0 24 0.29 +1Score > 41 indicates identity
Score > 31 indicates homology		 U R.DHFDFVTK.M
1107   336.8323 1007.4751 1007.4713 3.78 0 31 0.07 +1Score > 42 indicates identity
Score > 31 indicates homology		 U R.DHFDFVTK.M
1266 +1 543.7866 1085.5586 1085.5505 7.47 1 30 0.59 +1Score > 41 indicates identity
Score > 40 indicates homology		 U R.SWLEGLEPR.H
1267   1086.5664 1085.5591 1085.5505 7.91 1 61 0.00056 +1Score > 41 indicates identity U R.SWLEGLEPR.H
1283   1095.5823 1094.5750 1094.5720 2.76 0 59 0.00057 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1284 +1 548.2960 1094.5774 1094.5720 4.97 0 48 0.0072 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1627   623.3628 1244.7110 1244.7017 7.54 1 16 1 +1Score > 37 indicates identity
Score > 29 indicates homology		 U R.DLVTVFPEVVK.E
1692   640.8637 1279.7128 1279.7023 8.21 1 49 0.0036 +1Score > 37 indicates identity U R.QETLLTTAIYK.F
1943 +4 701.4050 1400.7954 1400.7776 12.8 0 77 2.7e-006 +1Score > 37 indicates identity
Score > 34 indicates homology		 U R.QAIGQLAQNLFAK.G
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.raw

Score > 41 indicates identity

Score > 20 indicates homology
9.87 1 8 0.97 1 ARDAAQMVADLVK   + Deamidated (NQ); Oxidation (M)
1.41 1 8 1 2 HVYPRNYIVNK   + 2 Deamidated (NQ)
-13.6 1 7 1.1 3 QPNGIISQYRVK   + 2 Deamidated (NQ)
-8.05 1 7 1.3 4 ILENGSVMALDVK   + Oxidation (M)
1950   351.9377 1403.7217 1403.7296 -5.63 0 6 1.4 -5Score > 41 indicates identity
Score > 20 indicates homology		 U R.QAIGQLAQNLFAK.G + 3 Deamidated (NQ)
6.98 1 6 1.6 6 MPSFGIGVAGPEVK   + Oxidation (M)
-1.04 0 6 1.6 7 TVAHGGTVMFVGTK  
-5.65 1 6 1.6 8 DAPDLIFATAKDK  
11.3 1 5 1.9 9 WPFSRQQNVSR  
19.3 0 4 2.6 10 NFHQHQITHLK   + 2 Deamidated (NQ)
2293   785.9303 1569.8460 1569.8363 6.24 1 79 5.1e-007 +1Score > 39 indicates identity
Score > 28 indicates homology		 U K.ITPDTVGVGLTNEVR.S
2294   524.2896 1569.8470 1569.8363 6.82 1 38 0.0031 +1Score > 39 indicates identity
Score > 25 indicates homology		 U K.ITPDTVGVGLTNEVR.S
2299   786.4144 1570.8142 1570.8203 -3.83 1 12 1.2 +2Score > 40 indicates identity
Score > 25 indicates homology		 U K.ITPDTVGVGLTNEVR.S + Deamidated (NQ)
3137   680.0335 2037.0787 2037.0565 10.9 1 59 1.8e-005 +1Score > 37 indicates identity
Score > 24 indicates homology		 U R.GVDVLEMHNLLTDIVQNK.E
3138   1019.5505 2037.0864 2037.0565 14.7 1 98 1.5e-008 +1Score > 37 indicates identity
Score > 32 indicates homology		 U R.GVDVLEMHNLLTDIVQNK.E
3145   680.3667 2038.0783 2038.0405 18.5 1 37 0.00094 +1Score > 37 indicates identity
Score > 19 indicates homology		 U R.GVDVLEMHNLLTDIVQNK.E + Deamidated (NQ)
3792   816.0698 2445.1876 2445.1448 17.5 1 68 7.1e-005 +1Score > 39 indicates identity U R.EQWDDGNNVVAIEPGVVIGYDR.N + Deamidated (NQ)
4075   904.4351 2710.2835 2710.3214 -14.0 0 0 0.99 +1Score > 37 indicates identity
Score > 13 indicates homology		 U K.MYNDYLGHSSFILPPLPNTQFTR.D
4076   904.7932 2711.3578 2711.3054 19.3 0 21 0.037 +1Score > 37 indicates identity
Score > 19 indicates homology		 U K.MYNDYLGHSSFILPPLPNTQFTR.D + Deamidated (NQ)

4 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
ARCA_PSEPU 130 46933 4.1
Arginine deiminase OS=Pseudomonas putida GN=arcA PE=1 SV=1
ARCA1_RHIME 84 46140 4.1
Arginine deiminase 1 OS=Rhizobium meliloti GN=arcA1 PE=3 SV=1
2 samesets of ARCA1_RHIME
ARCA_RHIET 84 46140
Arginine deiminase OS=Rhizobium etli GN=arcA PE=3 SV=1
ARCA_RHIE6 84 46196
Arginine deiminase OS=Rhizobium etli (strain CIAT 652) GN=arcA PE=3 SV=1
ARCA_PSEAE 81 46806 4.1
Arginine deiminase OS=Pseudomonas aeruginosa GN=arcA PE=1 SV=2
ARCA2_RHIME 69 46880 4.1
Arginine deiminase 2 OS=Rhizobium meliloti GN=arcA2 PE=3 SV=1
6 samesets of ARCA2_RHIME
ARCA_BURMA 69 46422
Arginine deiminase OS=Burkholderia mallei GN=arcA PE=3 SV=1
ARCA_BURPS 69 46422
Arginine deiminase OS=Burkholderia pseudomallei GN=arcA PE=3 SV=1
ARCA_PSEMY 69 46334
Arginine deiminase OS=Pseudomonas mendocina (strain ymp) GN=arcA PE=3 SV=1
ARCA_BRAJA 69 46780
Arginine deiminase OS=Bradyrhizobium japonicum GN=arcA PE=3 SV=1
ARCA_MARMS 69 46613
Arginine deiminase OS=Marinomonas sp. (strain MWYL1) GN=arcA PE=3 SV=1
ARCA_RHILO 69 46031
Arginine deiminase OS=Rhizobium loti GN=arcA PE=3 SV=1

+5

Accession Score Description
1 OTCC_PSEPK 391 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+6

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 350 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
2 RPOC_PSEA7 244 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 FTSZ_PSEPK 95 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+7

Accession Score Description
1 DLDH2_PSEPU 349 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+8

Accession Score Description
1 ADHP_ECOLI 327 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+9

Accession Score Description
1 ACON2_PSEAE 321 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+10

Accession Score Description
1 SUCC_PSEPK 313 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
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