MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURA_PSEPG | 138 | Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 138 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 136 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 132 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO_PSEPK | 132 | Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_PSEAE | 130 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 125 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 124 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 38.1 | RS5_PSEE4 |
124 | 17700 | 6 (4) | 5 (3) | 0.46 | |
| 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 | |||||||
| 4 samesets of RS5_PSEE4 | |||||||
| RS5_PSEP1 |
124 | 17712 | 6 (4) | 5 (3) | 0.45 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsE PE=3 SV=1 | |||||||
| RS5_PSEPG |
124 | 17712 | 6 (4) | 5 (3) | 0.45 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain GB-1) GN=rpsE PE=3 SV=1 | |||||||
| RS5_PSEPK |
124 | 17712 | 6 (4) | 5 (3) | 0.45 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1 | |||||||
| RS5_PSEPW |
124 | 17712 | 6 (4) | 5 (3) | 0.45 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain W619) GN=rpsE PE=3 SV=1 | |||||||
6 peptide matches (5 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 455 |
364.7259 | 727.4372 | 727.4341 | 4.37 | 0 | 19 | 0.25 | 1Score > 39 indicates identity Score > 25 indicates homology |
K.LVQVNR.V | ||
| 741 |
428.2509 | 854.4872 | 854.4861 | 1.29 | 1 | 18 | 0.4 | 1Score > 37 indicates identity Score > 27 indicates homology |
R.EVPAAIQK.A | ||
| 1047 |
491.7664 | 981.5182 | 981.5131 | 5.24 | 0 | 42 | 0.03 | 1Score > 39 indicates identity |
U | K.GTTLQYATK.A | |
| 1959 |
1 |
705.9235 | 1409.8324 | 1409.8242 | 5.85 | 1 | 51 | 0.00025 | 1Score > 33 indicates identity Score > 28 indicates homology |
U | R.AVLEVAGVQNVLAK.C |
| 2073 |
734.9124 | 1467.8102 | 1467.7974 | 8.78 | 0 | 78 | 1e-006 | 1Score > 38 indicates identity Score > 31 indicates homology |
U | R.IFTFTALTVVGDGK.G |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RS5_AZOVD |
118 | 17763 | 38.1 | ||
| 30S ribosomal protein S5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsE PE=3 SV=1 | |||||
| 7 samesets of RS5_AZOVD | |||||
| RS5_PSE14 |
118 | 17881 | |||
| 30S ribosomal protein S5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEF5 |
118 | 17699 | |||
| 30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEFS |
118 | 17732 | |||
| 30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEMY |
118 | 17708 | |||
| 30S ribosomal protein S5 OS=Pseudomonas mendocina (strain ymp) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEPF |
118 | 17769 | |||
| 30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSESM |
118 | 17778 | |||
| 30S ribosomal protein S5 OS=Pseudomonas syringae pv. tomato GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEU2 |
118 | 17778 | |||
| 30S ribosomal protein S5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEU5 |
78 | 17649 | 38.1 | ||
| 30S ribosomal protein S5 OS=Pseudomonas stutzeri (strain A1501) GN=rpsE PE=3 SV=1 | |||||
| RS5_ALCBS |
69 | 17601 | 38.1 | ||
| 30S ribosomal protein S5 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsE PE=3 SV=1 | |||||
| 5 samesets of RS5_ALCBS | |||||
| RS5_PSEA7 |
69 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEA8 |
69 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEAB |
69 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEAE |
69 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa GN=rpsE PE=3 SV=1 | |||||
| RS5_HAHCH |
69 | 17639 | |||
| 30S ribosomal protein S5 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsE PE=3 SV=1 | |||||
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_PSEPG | 122 | Phosphoglycerate kinase OS=Pseudomonas putida (strain GB-1) GN=pgk PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HYDA_PSEPU | 120 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2 | |
| 2 | HYDA_PSEAE | 115 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1 | |
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