MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURA_PSEPG | 138 | Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 138 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 136 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 132 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO_PSEPK | 132 | Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_PSEAE | 130 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 125 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 37.1 | EFTS_PSEPK |
125 | 30527 | 10 (7) | 8 (5) | 0.67 | |
| Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 | |||||||
| 4 samesets of EFTS_PSEPK | |||||||
| EFTS_PSEPW |
125 | 30452 | 10 (7) | 8 (5) | 0.67 | ||
| Elongation factor Ts OS=Pseudomonas putida (strain W619) GN=tsf PE=3 SV=1 | |||||||
| EFTS_PSEP1 |
125 | 30513 | 10 (7) | 8 (5) | 0.67 | ||
| Elongation factor Ts OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tsf PE=3 SV=1 | |||||||
| EFTS_PSEE4 |
125 | 30581 | 10 (7) | 8 (5) | 0.66 | ||
| Elongation factor Ts OS=Pseudomonas entomophila (strain L48) GN=tsf PE=3 SV=1 | |||||||
| EFTS_PSEPG |
125 | 30483 | 9 (7) | 7 (5) | 0.67 | ||
| Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 593 |
394.7126 | 787.4106 | 787.4076 | 3.90 | 1 | 40 | 0.042 | 1Score > 44 indicates identity Score > 39 indicates homology |
U | K.GGDVELAK.N | |
| 620 |
399.7778 | 797.5410 | 797.5375 | 4.48 | 0 | 37 | 0.014 | 1Score > 30 indicates identity |
U | K.IGAVVVLK.G | |
| 676 |
415.2445 | 828.4744 | 828.4705 | 4.78 | 0 | 21 | 1.3 | 2Score > 42 indicates identity Score > 35 indicates homology |
E.VAAQVAAAK.Q + Deamidated (NQ) | ||
| 745 |
857.5497 | 856.5424 | 856.5382 | 4.96 | 0 | 46 | 0.0062 | 1Score > 36 indicates identity |
U | M.AAITAALVK.E | |
| 746 |
429.2786 | 856.5426 | 856.5382 | 5.22 | 0 | 33 | 0.024 | 1Score > 36 indicates identity Score > 29 indicates homology |
U | M.AAITAALVK.E | |
| 1432 |
581.3237 | 1160.6328 | 1160.6190 | 12.0 | 0 | 18 | 0.16 | 1Score > 41 indicates identity Score > 23 indicates homology |
U | K.NVFLQLNADK.I | |
| 1454 |
585.8295 | 1169.6444 | 1169.6404 | 3.45 | 1 | 12 | 4.4 | 4Score > 40 indicates identity Score > 31 indicates homology |
U | K.AGNVAAEGAIAVK.T | |
| 1807 |
666.8525 | 1331.6904 | 1331.6761 | 10.7 | 1 | 35 | 0.013 | 1Score > 41 indicates identity Score > 28 indicates homology |
U | K.AGAEIVSFTYFK.V | |
| 2050 |
486.5864 | 1456.7374 | 1456.7310 | 4.34 | 1 | 44 | 0.002 | 1Score > 40 indicates identity Score > 30 indicates homology |
U | R.VEGDVVGAYLHGNK.I | |
| 2051 |
729.3760 | 1456.7374 | 1456.7310 | 4.39 | 1 | 85 | 4.1e-007 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | R.VEGDVVGAYLHGNK.I |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| EFTS_PSEPF |
49 | 30511 | 37.1 | ||
| Elongation factor Ts OS=Pseudomonas fluorescens (strain Pf0-1) GN=tsf PE=3 SV=1 | |||||
| EFTS_PSE14 |
41 | 30560 | 37.1 | ||
| Elongation factor Ts OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=tsf PE=3 SV=1 | |||||
| 3 samesets of EFTS_PSE14 | |||||
| EFTS_PSEFS |
41 | 30398 | |||
| Elongation factor Ts OS=Pseudomonas fluorescens (strain SBW25) GN=tsf PE=3 SV=1 | |||||
| EFTS_PSESM |
41 | 30620 | |||
| Elongation factor Ts OS=Pseudomonas syringae pv. tomato GN=tsf PE=3 SV=1 | |||||
| EFTS_PSEU2 |
41 | 30561 | |||
| Elongation factor Ts OS=Pseudomonas syringae pv. syringae (strain B728a) GN=tsf PE=3 SV=1 | |||||
| CLPX1_METCA |
40 | 46864 | 37.1 | ||
| ATP-dependent Clp protease ATP-binding subunit clpX 1 OS=Methylococcus capsulatus GN=clpX1 PE=3 SV=1 | |||||
| 2 samesets of CLPX1_METCA | |||||
| CLPX_AGRVS |
40 | 47206 | |||
| ATP-dependent Clp protease ATP-binding subunit clpX OS=Agrobacterium vitis (strain S4 / ATCC BAA-846) GN=clpX PE=3 SV=1 | |||||
| YLXY_BACSU |
40 | 36442 | |||
| Uncharacterized protein ylxY OS=Bacillus subtilis GN=ylxY PE=3 SV=2 | |||||
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 124 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_PSEPG | 122 | Phosphoglycerate kinase OS=Pseudomonas putida (strain GB-1) GN=pgk PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HYDA_PSEPU | 120 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2 | |
| 2 | HYDA_PSEAE | 115 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1 | |
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