MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 250)


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+31

Accession Score Description
1 PURA_PSEPG 138 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

+32

Accession Score Description
1 NUOG_PSEPK 138 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

+33

Accession Score Description
1 RL18_PSEP1 136 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+34

Accession Score Description
1 SUCD_PSEAE 132 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+35

Accession Score Description
1 ENO_PSEPK 132 Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1

-36

Accession Score Description
1 ODP2_PSEAE 130 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2
Score Mass Matches Sequences emPAI
36.1 ODP2_PSEAE 130 56788 7 (4) 6 (4) 0.17
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
133 +1 473.3115 472.3042 472.3009 6.97 0 23 1.4 +3Score > 45 indicates identity
Score > 37 indicates homology
K.AGVVK.S
706   421.7472 841.4798 841.4770 3.42 0 59 0.00049 +1Score > 39 indicates identity U R.VINGAAAAR.F
1112   505.3601 1008.7056 1008.6947 10.9 0 44 4.1e-005 +1Score > 13 indicates identity U K.LTVLPILLK.A
1531   403.8903 1208.6491 1208.6448 3.54 0 32 0.074 +1Score > 39 indicates identity
Score > 33 indicates homology
U R.LMQVGAANLHR.S
2032   482.2634 1443.7684 1443.7544 9.68 0 40 0.0014 +1Score > 40 indicates identity
Score > 24 indicates homology
U R.LMLPLSLSYDHR.V
2060   731.3694 1460.7242 1460.7147 6.53 1 71 1.9e-005 +1Score > 41 indicates identity
Score > 37 indicates homology
U K.ELPDFNSSLAPSGK.A

2 subsets and intersections (2 subset proteins in total)

Score Mass Subset of
ODP2_AZOVI 93 65119 36.1
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Azotobacter vinelandii PE=1 SV=3
ODP2_BUCBP 40 46148 36.1
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=aceF PE=3 SV=1

+37

Accession Score Description
1 EFTS_PSEPK 125 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1

+38

Accession Score Description
1 RS5_PSEE4 124 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+39

Accession Score Description
1 PGK_PSEPG 122 Phosphoglycerate kinase OS=Pseudomonas putida (strain GB-1) GN=pgk PE=3 SV=1

+40

Accession Score Description
Family member distances as a dendrogram 1 HYDA_PSEPU 120 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2
2 HYDA_PSEAE 115 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1
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