MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 250)

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+31

Accession Score Description
1 PURA_PSEPG 138 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

+32

Accession Score Description
1 NUOG_PSEPK 138 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

+33

Accession Score Description
1 RL18_PSEP1 136 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

-34

Accession Score Description
1 SUCD_PSEAE 132 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2
Score Mass Matches Sequences emPAI
34.1 SUCD_PSEAE 132 30646 11 (7) 8 (5) 0.55
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

-11 peptide matches (10 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
267   315.7022 629.3898 629.3861 6.01 0 19 1.5 +3Score > 41 indicates identity
Score > 33 indicates homology		 K.VGIVSR.S
414   703.4024 702.3951 702.3912 5.60 0 55 0.0034 +1Score > 43 indicates identity U R.SLADIGK.A
415   352.2050 702.3954 702.3912 6.06 0 28 1.5 +2Score > 43 indicates identity
Score > 42 indicates homology		 U R.SLADIGK.A
715   423.2321 844.4496 844.4477 2.32 0 21 0.7 +1Score > 44 indicates identity
Score > 32 indicates homology		 K.MVGGVTPGK.G
1149   510.2815 1018.5484 1018.5447 3.64 0 25 0.51 +1Score > 41 indicates identity
Score > 34 indicates homology		 U K.FAALQDAGVK.T
1238   534.7851 1067.5556 1067.5499 5.40 1 73 8e-006 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.SGTLTYEAVK.Q
1566   610.8384 1219.6622 1219.6448 14.3 1 57 0.001 +1Score > 40 indicates identity U K.DSILEAAFGGIK.L
1877   343.2018 1368.7781 1368.7700 5.91 0 45 0.0022 +1Score > 37 indicates identity
Score > 31 indicates homology		 U K.IGIMPGHIHLPGK.V
2229 +1 514.2893 1539.8461 1539.8410 3.32 0 27 0.034 +1Score > 38 indicates identity
Score > 25 indicates homology		 U K.GGTTHLGLPVFNTVK.E
2230   770.9341 1539.8536 1539.8410 8.24 0 34 0.0022 +1Score > 37 indicates identity
Score > 20 indicates homology		 U K.GGTTHLGLPVFNTVK.E

2 subsets and intersections (24 subset proteins in total)

Score Mass Subset of
SUCD_ECO57 73 30044 34.1
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O157:H7 GN=sucD PE=3 SV=2
3 samesets of SUCD_ECO57
SUCD_ECOLI 73 30044
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli (strain K12) GN=sucD PE=1 SV=2
SUCD_ECOL6 73 30044
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O6 GN=sucD PE=3 SV=2
SUCD_HAEIN 73 30769
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Haemophilus influenzae GN=sucD PE=3 SV=1
DNAA_RICAE 55 53103 34.1
Chromosomal replication initiator protein dnaA OS=Rickettsia africae (strain ESF-5) GN=dnaA PE=3 SV=1
19 samesets of DNAA_RICAE
DNAA_RICAH 55 53188
Chromosomal replication initiator protein dnaA OS=Rickettsia akari (strain Hartford) GN=dnaA PE=3 SV=1
DNAA_RICCK 55 53178
Chromosomal replication initiator protein dnaA OS=Rickettsia canadensis (strain McKiel) GN=dnaA PE=3 SV=1
DNAA_RICCN 55 53080
Chromosomal replication initiator protein dnaA OS=Rickettsia conorii GN=dnaA PE=3 SV=1
DNAA_RICFE 55 53070
Chromosomal replication initiator protein dnaA OS=Rickettsia felis GN=dnaA PE=3 SV=1
DNAA_RICPR 55 53143
Chromosomal replication initiator protein dnaA OS=Rickettsia prowazekii GN=dnaA PE=3 SV=1
DNAA_RICPU 55 53052
Chromosomal replication initiator protein dnaA OS=Rickettsia peacockii (strain Rustic) GN=dnaA PE=3 SV=1
DNAA_RICRO 55 53080
Chromosomal replication initiator protein dnaA OS=Rickettsia rickettsii (strain Iowa) GN=dnaA PE=3 SV=1
DNAA_RICRS 55 53080
Chromosomal replication initiator protein dnaA OS=Rickettsia rickettsii (strain Sheila Smith) GN=dnaA PE=3 SV=1
DNAA_RICTY 55 53188
Chromosomal replication initiator protein dnaA OS=Rickettsia typhi GN=dnaA PE=3 SV=1
S4A4_AMBTI 55 116888
Electrogenic sodium bicarbonate cotransporter 1 OS=Ambystoma tigrinum GN=SLC4A4 PE=2 SV=1
S4A4_BOVIN 55 122108
Electrogenic sodium bicarbonate cotransporter 1 OS=Bos taurus GN=SLC4A4 PE=1 SV=1
S4A4_HUMAN 55 122295
Electrogenic sodium bicarbonate cotransporter 1 OS=Homo sapiens GN=SLC4A4 PE=1 SV=1
S4A4_MOUSE 55 122318
Electrogenic sodium bicarbonate cotransporter 1 OS=Mus musculus GN=Slc4a4 PE=1 SV=2
S4A4_PIG 55 122187
Electrogenic sodium bicarbonate cotransporter 1 OS=Sus scrofa GN=SLC4A4 PE=1 SV=1
S4A4_RABIT 55 122204
Electrogenic sodium bicarbonate cotransporter 1 OS=Oryctolagus cuniculus GN=SLC4A4 PE=1 SV=1
S4A4_RAT 55 122178
Electrogenic sodium bicarbonate cotransporter 1 OS=Rattus norvegicus GN=Slc4a4 PE=1 SV=1
S4A5_HUMAN 55 127258
Electrogenic sodium bicarbonate cotransporter 4 OS=Homo sapiens GN=SLC4A5 PE=2 SV=2
S4A5_RAT 55 124721
Electrogenic sodium bicarbonate cotransporter 4 OS=Rattus norvegicus GN=Slc4a5 PE=1 SV=1
DNAA_RICM5 55 53008
Chromosomal replication initiator protein dnaA OS=Rickettsia massiliae (strain Mtu5) GN=dnaA PE=3 SV=1

+35

Accession Score Description
1 ENO_PSEPK 132 Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1

+36

Accession Score Description
1 ODP2_PSEAE 130 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+37

Accession Score Description
1 EFTS_PSEPK 125 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1

+38

Accession Score Description
1 RS5_PSEE4 124 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+39

Accession Score Description
1 PGK_PSEPG 122 Phosphoglycerate kinase OS=Pseudomonas putida (strain GB-1) GN=pgk PE=3 SV=1

+40

Accession Score Description
Family member distances as a dendrogram 1 HYDA_PSEPU 120 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2
2 HYDA_PSEAE 115 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1
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