MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 250)


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+31

Accession Score Description
1 PURA_PSEPG 138 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

+32

Accession Score Description
1 NUOG_PSEPK 138 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

-33

Accession Score Description
1 RL18_PSEP1 136 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
Score Mass Matches Sequences emPAI
33.1 RL18_PSEP1 136 12677 3 (3) 3 (3) 0.68
50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
3 samesets of RL18_PSEP1
RL18_PSEPG 136 12677 3 (3) 3 (3) 0.68
50S ribosomal protein L18 OS=Pseudomonas putida (strain GB-1) GN=rplR PE=3 SV=1
RL18_PSEPK 136 12677 3 (3) 3 (3) 0.68
50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1
RL18_PSEPW 136 12665 3 (3) 3 (3) 0.68
50S ribosomal protein L18 OS=Pseudomonas putida (strain W619) GN=rplR PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1328   560.7927 1119.5708 1119.5673 3.19 0 73 3.2e-005 +1Score > 42 indicates identity
Score > 40 indicates homology
U K.AAGVSQVAFDR.S
1361   567.2830 1132.5514 1132.5360 13.6 0 41 0.003 +1Score > 42 indicates identity
Score > 28 indicates homology
U R.DGATGNIDAATK.V
1911   463.5983 1387.7731 1387.7671 4.32 1 92 1.6e-007 +1Score > 38 indicates identity
Score > 36 indicates homology
U K.VLASASTLDKDLR.D

2 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
RL18_PSEA7 92 12711 33.1
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain PA7) GN=rplR PE=3 SV=1
4 samesets of RL18_PSEA7
RL18_PSEA8 92 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplR PE=3 SV=1
RL18_PSEAB 92 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplR PE=3 SV=1
RL18_PSEAE 92 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa GN=rplR PE=3 SV=1
RL18_TERTT 92 12760
50S ribosomal protein L18 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rplR PE=3 SV=1
RL18_PSE14 79 12653 33.1
50S ribosomal protein L18 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplR PE=3 SV=1
6 samesets of RL18_PSE14
RL18_PSEE4 79 12651
50S ribosomal protein L18 OS=Pseudomonas entomophila (strain L48) GN=rplR PE=3 SV=1
RL18_PSEF5 79 12692
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplR PE=3 SV=1
RL18_PSEFS 79 12708
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain SBW25) GN=rplR PE=3 SV=1
RL18_PSEPF 79 12692
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplR PE=3 SV=1
RL18_PSESM 79 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. tomato GN=rplR PE=3 SV=1
RL18_PSEU2 79 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplR PE=3 SV=1

+34

Accession Score Description
1 SUCD_PSEAE 132 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+35

Accession Score Description
1 ENO_PSEPK 132 Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1

+36

Accession Score Description
1 ODP2_PSEAE 130 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+37

Accession Score Description
1 EFTS_PSEPK 125 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1

+38

Accession Score Description
1 RS5_PSEE4 124 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+39

Accession Score Description
1 PGK_PSEPG 122 Phosphoglycerate kinase OS=Pseudomonas putida (strain GB-1) GN=pgk PE=3 SV=1

+40

Accession Score Description
Family member distances as a dendrogram 1 HYDA_PSEPU 120 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2
2 HYDA_PSEAE 115 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1
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