MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURA_PSEPG | 138 | Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 138 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 32.1 | NUOG_PSEPK |
138 | 98547 | 8 (4) | 8 (4) | 0.10 | |
| NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 676 |
415.2445 | 828.4744 | 828.4705 | 4.78 | 0 | 29 | 0.2 | 1Score > 42 indicates identity Score > 35 indicates homology |
K.VDAALAAAK.V | ||
| 995 |
479.2559 | 956.4972 | 956.4927 | 4.75 | 0 | 9 | 1.5 | 6Score > 40 indicates identity Score > 23 indicates homology |
U | R.QPQLADGTK.L | |
| 1146 |
508.8151 | 1015.6156 | 1015.6066 | 8.93 | 0 | 19 | 0.062 | 1Score > 34 indicates identity Score > 20 indicates homology |
U | R.IPAAYVALAK.S | |
| 1313 |
555.2850 | 1108.5554 | 1108.5513 | 3.78 | 1 | 27 | 0.94 | 3Score > 41 indicates identity Score > 39 indicates homology |
U | R.ASLESNYGLR.E | |
| 2559 |
844.4472 | 1686.8798 | 1686.8630 | 9.97 | 0 | 46 | 0.00062 | 1Score > 40 indicates identity Score > 26 indicates homology |
U | R.LNWFNAIPGAFNPAR.G | |
| 2836 |
916.5521 | 1831.0896 | 1831.0567 | 18.0 | 0 | 50 | 0.00023 | 1Score > 26 indicates identity |
U | R.LALNVLNNSPLPVPTLR.D + Deamidated (NQ) | |
| 3707 |
788.1306 | 2361.3700 | 2361.3267 | 18.3 | 1 | 40 | 0.00033 | 1Score > 27 indicates identity Score > 18 indicates homology |
U | K.RPLVVAGTSLADPALIEAAANIAK.A + Deamidated (NQ) | |
| 4265 |
959.1730 | 2874.4972 | 2874.4512 | 16.0 | 1 | 69 | 6e-007 | 1Score > 36 indicates identity Score > 20 indicates homology |
U | R.IGFAVAHAIDPSAPAVAGLDDEAQALAQR.I + Deamidated (NQ) |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 136 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 132 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO_PSEPK | 132 | Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_PSEAE | 130 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 125 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 124 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_PSEPG | 122 | Phosphoglycerate kinase OS=Pseudomonas putida (strain GB-1) GN=pgk PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HYDA_PSEPU | 120 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2 | |
| 2 | HYDA_PSEAE | 115 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1 | |
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