MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 250)

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+21

Accession Score Description
1 ACCA_PSEPK 181 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1

+22

Accession Score Description
1 PORF_PSESY 180 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+23

Accession Score Description
1 HTPG_PSEPK 173 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+24

Accession Score Description
1 RL1_PSESM 172 50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1

+25

Accession Score Description
1 ETFB_PSEAE 160 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+26

Accession Score Description
1 TIG_PSEPK 154 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2

+27

Accession Score Description
1 IDH_AZOVI 146 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+28

Accession Score Description
1 GUAA_CELJU 142 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+29

Accession Score Description
1 SYR_PSEPK 139 Arginyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=argS PE=3 SV=1

-30

Accession Score Description
1 RL11_PSEP1 138 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
Score Mass Matches Sequences emPAI
30.1 RL11_PSEP1 138 14914 4 (2) 4 (2) 0.34
50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
3 samesets of RL11_PSEP1
RL11_PSEPG 138 14914 4 (2) 4 (2) 0.34
50S ribosomal protein L11 OS=Pseudomonas putida (strain GB-1) GN=rplK PE=3 SV=1
RL11_PSEPK 138 14914 4 (2) 4 (2) 0.34
50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1
RL11_PSEPW 138 14914 4 (2) 4 (2) 0.34
50S ribosomal protein L11 OS=Pseudomonas putida (strain W619) GN=rplK PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
751   430.2152 858.4158 858.4123 4.09 1 18 0.4 +1Score > 40 indicates identity
Score > 27 indicates homology		 R.SFTFETK.S
887   458.2812 914.5478 914.5437 4.57 0 7 1.4 +10Score > 40 indicates identity
Score > 21 indicates homology		 K.STPASVLLK.K
1997   715.3717 1428.7288 1428.7209 5.59 0 85 1.8e-006 +1Score > 41 indicates identity U K.QADLTAADLDAAVR.T
3329   1073.5737 2145.1328 2145.0954 17.5 1 88 6.2e-008 +1Score > 37 indicates identity
Score > 29 indicates homology		 U R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ)

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
RL11_CHRVO 85 15002 30.1
50S ribosomal protein L11 OS=Chromobacterium violaceum GN=rplK PE=3 SV=1
6 samesets of RL11_CHRVO
RL11_PSEE4 85 14940
50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1
RL11_PSEMY 85 14959
50S ribosomal protein L11 OS=Pseudomonas mendocina (strain ymp) GN=rplK PE=3 SV=1
RL11_PSEA7 85 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain PA7) GN=rplK PE=3 SV=1
RL11_PSEA8 85 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplK PE=3 SV=1
RL11_PSEAB 85 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplK PE=3 SV=1
RL11_PSEAE 85 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa GN=rplK PE=3 SV=1
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