MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 250)

Page: 1 2 3 4 5 6 … 25 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1177 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_CARRP 73 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1045 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 EFTU_BACAA 271 Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
4 EFTU_ANATD 83 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 525 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
5 ATPG_RHOP5 40 description
3 ATPB_LEGPA 167 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
2 CH60_PSEPK 473 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
6 CH602_PROMS 39 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1
4 CH602_SINMW 59 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPG 522 ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 ATPA_RICAH 112 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
ATPA_PSEPG 522 55460 35 (22) 23 (14) 1.07
ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 samesets of ATPA_PSEPG
ATPA_PSEPK 522 55489 35 (22) 23 (14) 1.07
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
ATPA_PSEP1 522 55458 35 (22) 23 (14) 1.07
ATP synthase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpA PE=3 SV=1
ATPA_RICAH 112 56389 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
7 samesets of ATPA_RICAH
ATPA_RICCN 112 56128 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia conorii GN=atpA PE=3 SV=2
ATPA_RICFE 112 56172 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia felis GN=atpA PE=3 SV=1
ATPA_RICPU 112 56160 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia peacockii (strain Rustic) GN=atpA PE=3 SV=1
ATPA_RICRO 112 56195 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Iowa) GN=atpA PE=3 SV=2
ATPA_RICRS 112 56195 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Sheila Smith) GN=atpA PE=3 SV=1
ATPA_RICM5 112 56127 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia massiliae (strain Mtu5) GN=atpA PE=3 SV=2
ATPA_RICB8 112 56616 9 (6) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia bellii (strain OSU 85-389) GN=atpA PE=3 SV=1

-36 peptide matches (30 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
220 +1 301.6859 601.3572 601.3548 4.15 0 31 0.3 +1Score > 43 indicates identity
Score > 39 indicates homology		 X K.LSGGIR.T
227   606.2903 605.2830 605.2809 3.46 0 12 0.17 +1Score > 40 indicates identity
Score > 17 indicates homology		 X K.ATQTW.-
275   318.1805 634.3464 634.3438 4.11 0 32 0.043 +1Score > 41 indicates identity
Score > 31 indicates homology		 U X E.LAQFR.E + Deamidated (NQ)
420   707.3874 706.3801 706.3762 5.55 0 29 0.65 +1Score > 39 indicates identity X K.QAVAYR.Q
441   360.7031 719.3916 719.3887 4.03 1 29 0.14 +1Score > 41 indicates identity
Score > 33 indicates homology		 X R.VTELMK.Q
656   408.2363 814.4580 814.4548 3.93 1 24 1.3 +4Score > 43 indicates identity
Score > 38 indicates homology		 X X R.ELIIGDR.Q
657   815.4656 814.4583 814.4548 4.27 1 38 0.19 +1Score > 43 indicates identity X X R.ELIIGDR.Q
664   411.7275 821.4404 821.4395 1.12 0 28 0.79 +1Score > 40 indicates identity
Score > 39 indicates homology		 X R.TALAQYR.E
710 +1 421.7791 841.5436 841.5385 6.10 0 43 0.017 +1Score > 37 indicates identity U X X R.QISLLLR.R
739 +1 427.7728 853.5310 853.5273 4.39 1 39 0.014 +1Score > 33 indicates identity U X R.ILEVPVGK.E
754   430.2552 858.4958 858.4923 4.12 0 30 0.12 +1Score > 42 indicates identity
Score > 33 indicates homology		 U X R.STVANIVR.K
755   859.5044 858.4971 858.4923 5.61 0 28 0.37 +1Score > 42 indicates identity
Score > 37 indicates homology		 U X R.STVANIVR.K
831   447.7429 893.4712 893.4607 11.8 0 32 0.029 +1Score > 40 indicates identity
Score > 29 indicates homology		 U X K.FTNGAVTGK.T
834   448.2314 894.4482 894.4447 3.96 0 39 0.011 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X K.FTNGAVTGK.T + Deamidated (NQ)
836   449.2691 896.5236 896.5232 0.47 0 45 0.0068 +1Score > 36 indicates identity U X K.VAPGVIWR.K
1202   522.7814 1043.5482 1043.5434 4.68 0 36 0.063 +1Score > 42 indicates identity
Score > 37 indicates homology		 U X K.SVDAMIPVGR.G
1510 +1 599.3305 1196.6464 1196.6401 5.30 0 64 0.00015 +1Score > 39 indicates identity U X X R.VVDALGNPIDGK.G
1556   609.3121 1216.6096 1216.6048 4.01 0 70 9.6e-005 +1Score > 42 indicates identity U X R.IDNLDVSSQAR.N
1568   611.3117 1220.6088 1220.6037 4.19 0 12 0.45 +1Score > 42 indicates identity
Score > 21 indicates homology		 X K.SVDQPVQTGYK.S
1710   644.8558 1287.6970 1287.6856 8.86 0 54 0.0026 +1Score > 40 indicates identity U X K.TAMAIDAIINQK.D
1717   647.3046 1292.5946 1292.5885 4.79 1 57 0.00014 +1Score > 40 indicates identity
Score > 31 indicates homology		 U X K.GDFNDEIDAGLK.A
1802   665.3373 1328.6600 1328.6572 2.14 1 96 2.4e-008 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X K.GPLGNTQTDAVEK.V
2001   715.8739 1429.7332 1429.7273 4.13 1 70 2.8e-005 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.GRIDNLDVSSQAR.N
2002   477.5853 1429.7341 1429.7273 4.71 1 37 0.034 +1Score > 41 indicates identity
Score > 35 indicates homology		 U X K.GRIDNLDVSSQAR.N
2004   716.3756 1430.7366 1430.7365 0.074 1 55 0.0014 +1Score > 41 indicates identity
Score > 39 indicates homology		 U X R.NEGTVVSVSDGIVR.I
2259   777.3774 1552.7402 1552.7310 5.93 1 69 8.9e-005 +1Score > 41 indicates identity U X X R.EAYPGDVFYLHSR.L
2260   518.5880 1552.7422 1552.7310 7.17 1 45 0.0056 +1Score > 41 indicates identity
Score > 35 indicates homology		 U X X R.EAYPGDVFYLHSR.L
2402   538.2944 1611.8614 1611.8409 12.7 1 72 2.9e-005 +1Score > 39 indicates identity U X K.IGSFEQALIAFFNR.D
2404 +2 806.9391 1611.8636 1611.8409 14.1 1 86 1e-007 +1Score > 39 indicates identity
Score > 29 indicates homology		 U X K.IGSFEQALIAFFNR.D
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.raw

Score > 39 indicates identity
3236   701.3495 2101.0267 2100.9859 19.4 1 71 3.6e-005 -1Score > 39 indicates identity U X K.QYAPMSIADMALSLYAAER.G + Deamidated (NQ)
15.9 0 23 2.5 2 HNADIIGSIVMAACTGNAIR   + 2 Deamidated (NQ); Oxidation (M)
14.0 0 15 15 3 FDCGVVIAADMLGSYGSLAR  
14.6 1 9 68 4 TVSVGSFLMVSEQLQMLSE   + Deamidated (NQ); Oxidation (M)
14.6 1 9 68 4 TVSVGSFLMVSEQLQMLSE   + Deamidated (NQ); Oxidation (M)
15.6 1 8 71 6 TVWHADVVQAMAGLGWSEK   + Deamidated (NQ); Oxidation (M)
-6.40 1 8 83 7 NSQAYDLLSKMILYDPSK   + Oxidation (M)
9.07 1 6 1.3e+002 8 SLANDGYRVVVSGTDQTYR   + Deamidated (NQ)
-17.1 1 6 1.3e+002 9 AGLQPRADMAAPPTLPQPPR   + 2 Deamidated (NQ); Oxidation (M)
5.13 0 6 1.4e+002 10 INTIMQTCFFALSGVMPR   + Oxidation (M)

+33 subsets and intersections (857 subset proteins in total)

+4

Accession Score Description
1 ARCA_PSEPK 463 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+5

Accession Score Description
1 OTCC_PSEPK 391 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+6

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 350 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
2 RPOC_PSEA7 244 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 FTSZ_PSEPK 95 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+7

Accession Score Description
1 DLDH2_PSEPU 349 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+8

Accession Score Description
1 ADHP_ECOLI 327 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+9

Accession Score Description
1 ACON2_PSEAE 321 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+10

Accession Score Description
1 SUCC_PSEPK 313 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
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