MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 241–250 (out of 250)


Page: Previous 1 20 21 22 23 24 25 

+241

Accession Score Description
1 FB236_ARATH 20 Putative F-box protein At4g17780 OS=Arabidopsis thaliana GN=At4g17780 PE=4 SV=1

+242

Accession Score Description
1 PEP1_YEAST 20 Vacuolar protein sorting/targeting protein PEP1 OS=Saccharomyces cerevisiae GN=PEP1 PE=1 SV=1

+243

Accession Score Description
1 SDG41_ARATH 20 Protein SET DOMAIN GROUP 41 OS=Arabidopsis thaliana GN=SDG41 PE=2 SV=1

+244

Accession Score Description
1 NUOB2_BURP1 19 Putative NADH-quinone oxidoreductase subunit B 2 OS=Burkholderia pseudomallei (strain 1710b) GN=nuoB2 PE=3 SV=2

+245

Accession Score Description
1 G6PI_BAUCH 18 Glucose-6-phosphate isomerase OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=pgi PE=3 SV=1

+246

Accession Score Description
1 DRL17_ARATH 18 Probable disease resistance protein At1g61300 OS=Arabidopsis thaliana GN=At1g61300 PE=2 SV=2

+247

Accession Score Description
1 YBC8_SCHPO 17 Uncharacterized protein C27B12.08 OS=Schizosaccharomyces pombe GN=SPBC27B12.08 PE=1 SV=1

-248

Accession Score Description
1 PAC_STRMU 17 Major cell-surface adhesin PAc OS=Streptococcus mutans GN=pac PE=1 SV=1
Score Mass Matches Sequences emPAI
248.1 PAC_STRMU 17 170735 3 (1) 2 (1) 0.01
Major cell-surface adhesin PAc OS=Streptococcus mutans GN=pac PE=1 SV=1

-3 peptide matches (2 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
645 +1 404.7155 807.4164 807.4126 4.71 0 16 2.3 +8Score > 42 indicates identity
Score > 32 indicates homology
K.YQADLAK.Y
3333   716.3820 2146.1242 2146.0906 15.7 1 17 0.029 +1Score > 38 indicates identity
Score > 14 indicates homology
U E.DEKPINFDNALLSVTSLNR.E + Deamidated (NQ)

+249

Accession Score Description
1 SRB8_USTMA 16 Mediator of RNA polymerase II transcription subunit 12 OS=Ustilago maydis GN=SRB8 PE=3 SV=1

+250

Accession Score Description
1 RPOC_THEMA 15 DNA-directed RNA polymerase subunit beta' OS=Thermotoga maritima GN=rpoC PE=3 SV=1
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