MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 231–240 (out of 250)


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+231

Accession Score Description
1 DAPF_PSEPG 27 Diaminopimelate epimerase OS=Pseudomonas putida (strain GB-1) GN=dapF PE=3 SV=1

-232

Accession Score Description
1 PEPT_STRTD 27 Peptidase T OS=Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) GN=pepT PE=3 SV=1
Score Mass Matches Sequences emPAI
232.1 PEPT_STRTD 27 45006 1 (1) 1 (1) 0.05
Peptidase T OS=Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) GN=pepT PE=3 SV=1
2 samesets of PEPT_STRTD
PEPT_STRT1 27 45009 1 (1) 1 (1) 0.05
Peptidase T OS=Streptococcus thermophilus (strain CNRZ 1066) GN=pepT PE=3 SV=1
PEPT_STRT2 27 45009 1 (1) 1 (1) 0.05
Peptidase T OS=Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) GN=pepT PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1956   470.5869 1408.7389 1408.7384 0.34 1 27 0.029 +1Score > 39 indicates identity
Score > 24 indicates homology
U E.IMTALAHLKANPE.I + Deamidated (NQ)

+233

Accession Score Description
1 BAS1_YEAST 26 Myb-like DNA-binding protein BAS1 OS=Saccharomyces cerevisiae GN=BAS1 PE=1 SV=1

+234

Accession Score Description
1 PYRC_PSEAB 26 Dihydroorotase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=pyrC PE=3 SV=1

+235

Accession Score Description
1 KPCD_CANFA 26 Protein kinase C delta type OS=Canis familiaris GN=PRKCD PE=2 SV=1

+236

Accession Score Description
1 HGD_PSEPK 23 Homogentisate 1,2-dioxygenase OS=Pseudomonas putida (strain KT2440) GN=hmgA PE=3 SV=1

+237

Accession Score Description
1 6PGL_PSEPU 23 6-phosphogluconolactonase OS=Pseudomonas putida GN=pgl PE=3 SV=1

+238

Accession Score Description
1 LSPA_BARHE 23 Lipoprotein signal peptidase OS=Bartonella henselae GN=lspA PE=3 SV=1

+239

Accession Score Description
1 SYT_HYPBU 21 Threonyl-tRNA synthetase OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403) GN=thrS PE=3 SV=1

+240

Accession Score Description
1 RPA34_SCHPO 21 DNA-directed RNA polymerase I subunit rpa34 OS=Schizosaccharomyces pombe GN=rpa34 PE=1 SV=1
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