MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACCA_PSEPK | 181 | Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 180 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 22.1 | PORF_PSESY |
180 | 36773 | 11 (5) | 4 (2) | 0.20 | |
| Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |||||||
11 peptide matches (6 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 335 |
2 |
336.1565 | 670.2984 | 670.2962 | 3.28 | 0 | 13 | 0.43 | 1Score > 37 indicates identity Score > 22 indicates homology |
K.FDFDK.S | |
| 338 |
671.3061 | 670.2988 | 670.2962 | 3.84 | 0 | 27 | 0.32 | 1Score > 37 indicates identity Score > 34 indicates homology |
K.FDFDK.S | ||
| 411 |
1 |
351.7063 | 701.3980 | 701.3959 | 2.99 | 1 | 27 | 1.2 | 2Score > 43 indicates identity Score > 41 indicates homology |
R.VELDVK.F | |
| 1746 |
1 |
436.2397 | 1305.6973 | 1305.6929 | 3.38 | 0 | 56 | 9.8e-005 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | K.SVVKPNSYGDIK.N |
| 1747 |
1 |
653.8561 | 1305.6976 | 1305.6929 | 3.67 | 0 | 63 | 0.00014 | 1Score > 40 indicates identity Score > 38 indicates homology |
U | K.SVVKPNSYGDIK.N |
| 3609 |
1152.0614 | 2302.1082 | 2302.0753 | 14.3 | 1 | 86 | 2.9e-007 | 1Score > 39 indicates identity Score > 33 indicates homology |
U | K.NNGNLFGGSIGYFLTDDVELR.L + 2 Deamidated (NQ) |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 173 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSESM | 172 | 50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ETFB_PSEAE | 160 | Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 154 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IDH_AZOVI | 146 | Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GUAA_CELJU | 142 | GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYR_PSEPK | 139 | Arginyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=argS PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 138 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
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