MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 211–220 (out of 250)


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+211

Accession Score Description
1 PUR5_PSE14 32 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=purM PE=3 SV=1

+212

Accession Score Description
1 HMDH3_ORYSJ 32 3-hydroxy-3-methylglutaryl-coenzyme A reductase 3 OS=Oryza sativa subsp. japonica GN=HMG3 PE=2 SV=2

+213

Accession Score Description
1 ILVC_METFK 31 Ketol-acid reductoisomerase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=ilvC PE=3 SV=1

+214

Accession Score Description
1 AK_PSEAE 31 Aspartokinase OS=Pseudomonas aeruginosa GN=lysC PE=3 SV=2

+215

Accession Score Description
1 NDC80_MACFA 30 Kinetochore protein NDC80 homolog OS=Macaca fascicularis GN=NDC80 PE=2 SV=1

+216

Accession Score Description
1 MRAW_AZOSB 30 S-adenosyl-L-methionine-dependent methyltransferase mraW OS=Azoarcus sp. (strain BH72) GN=mraW PE=3 SV=1

+217

Accession Score Description
1 AROC_PSEAB 30 Chorismate synthase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=aroC PE=3 SV=1

+218

Accession Score Description
1 METK_PSEPG 30 S-adenosylmethionine synthetase OS=Pseudomonas putida (strain GB-1) GN=metK PE=3 SV=1

-219

Accession Score Description
1 FRMA_SYNY3 29 S-(hydroxymethyl)glutathione dehydrogenase OS=Synechocystis sp. (strain PCC 6803) GN=frmA PE=3 SV=1
Score Mass Matches Sequences emPAI
219.1 FRMA_SYNY3 29 39813 1 (1) 1 (1) 0.06
S-(hydroxymethyl)glutathione dehydrogenase OS=Synechocystis sp. (strain PCC 6803) GN=frmA PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4274   962.8594 2885.5564 2885.5036 18.3 1 29 0.0018 +1Score > 33 indicates identity
Score > 14 indicates homology
U R.GWGQSVIIGVAGAGQEISTRPFQLVTGR.K + 2 Deamidated (NQ)

+220

Accession Score Description
1 DECR2_DANRE 29 Peroxisomal 2,4-dienoyl-CoA reductase OS=Danio rerio GN=decr2 PE=2 SV=1
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