MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 201–210 (out of 250)


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+201

Accession Score Description
1 COAX_ACICJ 34 Type III pantothenate kinase OS=Acidiphilium cryptum (strain JF-5) GN=coaX PE=3 SV=1

+202

Accession Score Description
1 GCSH2_PSEPK 33 Glycine cleavage system H protein 2 OS=Pseudomonas putida (strain KT2440) GN=gcvH2 PE=3 SV=1

+203

Accession Score Description
1 E1A_ADE02 33 Early E1A 32 kDa protein OS=Human adenovirus C serotype 2 PE=3 SV=1

-204

Accession Score Description
1 DHOM_PSEAE 33 Homoserine dehydrogenase OS=Pseudomonas aeruginosa GN=hom PE=3 SV=2
Score Mass Matches Sequences emPAI
204.1 DHOM_PSEAE 33 46423 1 (1) 1 (1) 0.05
Homoserine dehydrogenase OS=Pseudomonas aeruginosa GN=hom PE=3 SV=2

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1894   461.6009 1381.7809 1381.7718 6.60 1 33 0.0039 +1Score > 37 indicates identity
Score > 21 indicates homology
U K.ALIAVHGNEIFAK.A

+205

Accession Score Description
1 GREA_PSEPK 33 Transcription elongation factor greA OS=Pseudomonas putida (strain KT2440) GN=greA PE=3 SV=1

+206

Accession Score Description
1 SYL_SHEDO 33 Leucyl-tRNA synthetase OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=leuS PE=3 SV=2

+207

Accession Score Description
1 GABD_ECOLI 33 Succinate-semialdehyde dehydrogenase [NADP+] OS=Escherichia coli (strain K12) GN=gabD PE=3 SV=1

+208

Accession Score Description
1 GLNA_AZOVI 33 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+209

Accession Score Description
1 MSHR_LORTA 32 description

+210

Accession Score Description
1 RNH3_STRP1 32 Ribonuclease HIII OS=Streptococcus pyogenes serotype M1 GN=rnhC PE=3 SV=1
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