MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 250)


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+11

Accession Score Description
1 DBHB_PSEAE 311 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+12

Accession Score Description
1 RPOB_PSEPG 306 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1

+13

Accession Score Description
1 EFG1_PSEPK 280 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+14

Accession Score Description
1 DNAK_PSEPK 280 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+15

Accession Score Description
1 RISB_PSEPG 245 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+16

Accession Score Description
1 SAHH_PSEP1 221 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+17

Accession Score Description
1 SECB_PSEPG 208 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 192 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+19

Accession Score Description
1 CLPB_PSEPK 191 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

-20

Accession Score Description
1 PNP_PSEPK 188 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
Score Mass Matches Sequences emPAI
20.1 PNP_PSEPK 188 75192 11 (7) 9 (7) 0.23
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
2 samesets of PNP_PSEPK
PNP_PSEPU 188 75166 11 (7) 9 (7) 0.23
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida GN=pnp PE=3 SV=1
PNP_PSEP1 188 75139 11 (7) 9 (7) 0.23
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pnp PE=3 SV=1

+11 peptide matches (11 non-duplicate, 0 duplicate)

7 subsets and intersections (32 subset proteins in total)

Score Mass Subset of
PNP_PSEE4 171 75140 20.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas entomophila (strain L48) GN=pnp PE=3 SV=1
1 sameset of PNP_PSEE4
PNP_PSEPG 171 75207
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1
PNP_AZOVD 158 75912 20.1
Polyribonucleotide nucleotidyltransferase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=pnp PE=3 SV=1
PNP_PSE14 109 75121 20.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=pnp PE=3 SV=1
6 samesets of PNP_PSE14
PNP_PSEFS 109 75117
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain SBW25) GN=pnp PE=3 SV=1
PNP_PSEPF 109 75026
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf0-1) GN=pnp PE=3 SV=1
PNP_PSEPW 109 75250
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain W619) GN=pnp PE=3 SV=1
PNP_PSESM 109 75108
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. tomato GN=pnp PE=3 SV=1
PNP_PSEU2 109 75123
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=pnp PE=3 SV=1
PNP_PSEF5 109 75343
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=pnp PE=3 SV=1
PNP_PSEMY 96 75677 20.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas mendocina (strain ymp) GN=pnp PE=3 SV=2
1 sameset of PNP_PSEMY
PNP_PSEU5 96 75420
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=pnp PE=3 SV=1
PNP_PSEA7 79 75691 20.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain PA7) GN=pnp PE=3 SV=1
3 samesets of PNP_PSEA7
PNP_PSEAB 79 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=pnp PE=3 SV=1
PNP_PSEAE 79 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa GN=pnp PE=3 SV=1
PNP_PSEA8 79 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain LESB58) GN=pnp PE=3 SV=1
PNP_POLNS 65 77289 20.1
Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter necessarius (strain STIR1) GN=pnp PE=3 SV=1
1 sameset of PNP_POLNS
PNP_POLSQ 65 77293
Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=pnp PE=3 SV=1
PNP_MARAV 45 78217 20.1
Polyribonucleotide nucleotidyltransferase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=pnp PE=3 SV=1
13 samesets of PNP_MARAV
PNP_SHEAM 45 75438
Polyribonucleotide nucleotidyltransferase OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=pnp PE=3 SV=1
PNP_SHEFN 45 75279
Polyribonucleotide nucleotidyltransferase OS=Shewanella frigidimarina (strain NCIMB 400) GN=pnp PE=3 SV=1
PNP_SHEON 45 75605
Polyribonucleotide nucleotidyltransferase OS=Shewanella oneidensis GN=pnp PE=3 SV=2
PNP_SHEPC 45 75520
Polyribonucleotide nucleotidyltransferase OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=pnp PE=3 SV=1
PNP_SHESA 45 75432
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain ANA-3) GN=pnp PE=3 SV=1
PNP_SHESM 45 75355
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain MR-4) GN=pnp PE=3 SV=1
PNP_SHESR 45 75341
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain MR-7) GN=pnp PE=3 SV=1
PNP_SHESW 45 75520
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain W3-18-1) GN=pnp PE=3 SV=1
PNP_SHEB2 45 75322
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS223) GN=pnp PE=3 SV=1
PNP_SHEB5 45 75393
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=pnp PE=3 SV=2
PNP_SHEB8 45 75363
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS185) GN=pnp PE=3 SV=1
PNP_SHEB9 45 75393
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS195) GN=pnp PE=3 SV=1
PNP_PSEHT 45 75667
Polyribonucleotide nucleotidyltransferase OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=pnp PE=3 SV=1

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