MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 311 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 306 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 280 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 280 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 245 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEP1 | 221 | Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SECB_PSEPG | 208 | Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 192 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 191 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 19.1 | CLPB_PSEPK |
191 | 94917 | 13 (7) | 11 (6) | 0.18 | |
| Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 | |||||||
13 peptide matches (13 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 350 |
674.3494 | 673.3421 | 673.3395 | 3.93 | 1 | 24 | 0.62 | 1Score > 43 indicates identity Score > 34 indicates homology |
K.DAALER.R | ||
| 711 |
422.2570 | 842.4994 | 842.4974 | 2.42 | 0 | 36 | 0.11 | 1Score > 42 indicates identity Score > 39 indicates homology |
R.TVQVLQR.R | ||
| 781 |
436.2558 | 870.4970 | 870.4963 | 0.82 | 0 | 27 | 0.31 | 1Score > 40 indicates identity Score > 35 indicates homology |
K.WTGIPVAK.M | ||
| 1172 |
515.2899 | 1028.5652 | 1028.5614 | 3.71 | 1 | 64 | 0.00028 | 1Score > 41 indicates identity |
U | K.TAIAEGLAQR.I | |
| 1411 |
577.8276 | 1153.6406 | 1153.6343 | 5.52 | 1 | 48 | 0.00095 | 1Score > 38 indicates identity Score > 30 indicates homology |
U | R.IINGEVPDGLK.G | |
| 1722 |
647.3627 | 1292.7108 | 1292.7088 | 1.55 | 0 | 8 | 2.2 | 3Score > 38 indicates identity Score > 24 indicates homology |
K.NNPVLIGEPGVGK.T | ||
| 1849 |
678.9061 | 1355.7976 | 1355.7846 | 9.61 | 0 | 46 | 0.0035 | 1Score > 34 indicates identity |
U | R.LLALDMGALIAGAK.Y | |
| 2283 |
783.4644 | 1564.9142 | 1564.8977 | 10.6 | 1 | 52 | 4.4e-005 | 1Score > 32 indicates identity Score > 21 indicates homology |
U | R.WIENPLAQLILAGK.F | |
| 2348 |
529.2907 | 1584.8503 | 1584.8471 | 1.99 | 1 | 24 | 0.077 | 1Score > 39 indicates identity Score > 26 indicates homology |
U | R.VIGQSEAVTAVANAVR.R + Deamidated (NQ) | |
| 2349 |
793.4343 | 1584.8540 | 1584.8471 | 4.37 | 1 | 32 | 0.028 | 1Score > 39 indicates identity Score > 29 indicates homology |
U | R.VIGQSEAVTAVANAVR.R + Deamidated (NQ) | |
| 2633 |
861.9764 | 1721.9382 | 1721.9352 | 1.76 | 1 | 4 | 3.7 | 9Score > 37 indicates identity Score > 23 indicates homology |
U | E.KAHPDVFNVLLQVLE.D + Deamidated (NQ) | |
| 2954 |
961.5228 | 1921.0310 | 1921.0058 | 13.2 | 1 | 75 | 8e-006 | 1Score > 37 indicates identity |
U | K.AHPDVFNVLLQVLEDGR.L | |
| 2955 |
641.3535 | 1921.0387 | 1921.0058 | 17.1 | 1 | 69 | 2.3e-005 | 1Score > 36 indicates identity Score > 35 indicates homology |
U | K.AHPDVFNVLLQVLEDGR.L |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| CLPB_PSEAE |
89 | 95061 | 19.1 | ||
| Chaperone protein clpB OS=Pseudomonas aeruginosa GN=clpB PE=3 SV=1 | |||||
| CLPB1_SYNE7 |
72 | 98723 | 19.1 | ||
| Chaperone protein clpB 1 OS=Synechococcus elongatus (strain PCC 7942) GN=clpB1 PE=2 SV=3 | |||||
| 1 sameset of CLPB1_SYNE7 | |||||
| CLPB_PLEBO |
72 | 99301 | |||
| Chaperone protein clpB OS=Plectonema boryanum GN=clpB PE=2 SV=2 | |||||
| CLPAA_SOLLC |
64 | 102892 | 19.1 | ||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4A, chloroplastic OS=Solanum lycopersicum GN=CD4A PE=3 SV=1 | |||||
| 17 samesets of CLPAA_SOLLC | |||||
| CLPB2_SYNE7 |
64 | 99931 | |||
| Chaperone protein clpB 2 OS=Synechococcus elongatus (strain PCC 7942) GN=clpB2 PE=3 SV=1 | |||||
| CLPB2_THEEB |
64 | 99950 | |||
| Chaperone protein clpB 2 OS=Thermosynechococcus elongatus (strain BP-1) GN=clpB2 PE=3 SV=1 | |||||
| CLPB2_SYNY3 |
64 | 98119 | |||
| Chaperone protein clpB 2 OS=Synechocystis sp. (strain PCC 6803) GN=clpB2 PE=3 SV=1 | |||||
| CLPB1_THEEB |
64 | 98443 | |||
| Chaperone protein clpB 1 OS=Thermosynechococcus elongatus (strain BP-1) GN=clpB1 PE=3 SV=1 | |||||
| CLPB2_ANASP |
64 | 98620 | |||
| Chaperone protein clpB 2 OS=Anabaena sp. (strain PCC 7120) GN=clpB2 PE=3 SV=2 | |||||
| CLPB_GLOVI |
64 | 98196 | |||
| Chaperone protein clpB OS=Gloeobacter violaceus GN=clpB PE=3 SV=1 | |||||
| CLPB_PSESM |
64 | 95243 | |||
| Chaperone protein clpB OS=Pseudomonas syringae pv. tomato GN=clpB PE=3 SV=1 | |||||
| CLPC_CYACA |
64 | 95688 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Cyanidium caldarium GN=clpC PE=3 SV=1 | |||||
| CLPB_PARUW |
64 | 98713 | |||
| Chaperone protein clpB OS=Protochlamydia amoebophila (strain UWE25) GN=clpB PE=3 SV=1 | |||||
| CLPC_PORPU |
64 | 91062 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Porphyra purpurea GN=clpC PE=3 SV=1 | |||||
| CLPC_PORYE |
64 | 91042 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Porphyra yezoensis GN=clpC PE=3 SV=1 | |||||
| CLPB1_SYNY3 |
64 | 101443 | |||
| Chaperone protein clpB 1 OS=Synechocystis sp. (strain PCC 6803) GN=clpB1 PE=3 SV=1 | |||||
| CLPAB_SOLLC |
64 | 102463 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic OS=Solanum lycopersicum GN=CD4B PE=3 SV=1 | |||||
| CLPA_BRANA |
64 | 97430 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog, chloroplastic (Fragment) OS=Brassica napus GN=CLPA PE=2 SV=1 | |||||
| CLPC_GUITH |
64 | 90960 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Guillardia theta GN=clpC PE=3 SV=1 | |||||
| CLPC_PEA |
64 | 102818 | |||
| ATP-dependent Clp protease ATP-binding subunit clpC homolog, chloroplastic OS=Pisum sativum PE=2 SV=1 | |||||
| CLP_TRYBB |
64 | 97187 | |||
| Heat shock protein 100 OS=Trypanosoma brucei brucei GN=HSP100 PE=3 SV=1 | |||||
| CLPB_COXBU |
46 | 96823 | 19.1 | ||
| Chaperone protein clpB OS=Coxiella burnetii GN=clpB PE=3 SV=1 | |||||
| 1 sameset of CLPB_COXBU | |||||
| CLPB_RHOPA |
46 | 96669 | |||
| Chaperone protein clpB OS=Rhodopseudomonas palustris GN=clpB PE=3 SV=1 | |||||
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PNP_PSEPK | 188 | Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1 |
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