MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 181–190 (out of 250)


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+181

Accession Score Description
1 ACCD_BLOPB 37 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta OS=Blochmannia pennsylvanicus (strain BPEN) GN=accD PE=3 SV=1

+182

Accession Score Description
1 EIF3H_MONBE 37 Eukaryotic translation initiation factor 3 subunit H OS=Monosiga brevicollis GN=9558 PE=3 SV=1

+183

Accession Score Description
1 IDH1_COLMA 37 Isocitrate dehydrogenase [NADP] 1 OS=Colwellia maris GN=icdI PE=1 SV=2

-184

Accession Score Description
1 IF3_AZOVI 37 Translation initiation factor IF-3 OS=Azotobacter vinelandii GN=infC PE=3 SV=1
Score Mass Matches Sequences emPAI
184.1 IF3_AZOVI 37 20825 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Azotobacter vinelandii GN=infC PE=3 SV=1
10 samesets of IF3_AZOVI
IF3_PSEE4 37 20998 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas entomophila (strain L48) GN=infC PE=3 SV=1
IF3_PSEF5 37 20879 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=infC PE=3 SV=1
IF3_PSEFL 37 20879 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas fluorescens GN=infC PE=3 SV=1
IF3_PSEFS 37 20894 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas fluorescens (strain SBW25) GN=infC PE=3 SV=1
IF3_PSEP1 37 20923 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=infC PE=3 SV=1
IF3_PSEPG 37 20923 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas putida (strain GB-1) GN=infC PE=3 SV=1
IF3_PSEPK 37 20923 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas putida (strain KT2440) GN=infC PE=3 SV=2
IF3_PSEPW 37 20895 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas putida (strain W619) GN=infC PE=3 SV=1
IF3_PSESM 37 20850 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas syringae pv. tomato GN=infC PE=3 SV=1
IF3_PSESY 37 20850 1 (1) 1 (1) 0.11
Translation initiation factor IF-3 OS=Pseudomonas syringae pv. syringae GN=infC PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1261   542.7936 1083.5726 1083.5672 5.00 1 37 0.0074 +1Score > 40 indicates identity
Score > 28 indicates homology
U K.APINENISAR.E

+185

Accession Score Description
1 ODB2_PSEPU 37 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+186

Accession Score Description
1 SYE_PSEPG 36 Glutamyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=gltX PE=3 SV=1

+187

Accession Score Description
1 ATPB_BACWK 36 ATP synthase subunit beta OS=Bacillus weihenstephanensis (strain KBAB4) GN=atpD PE=3 SV=1

+188

Accession Score Description
1 ARLY_ACIC1 36 Argininosuccinate lyase OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) GN=argH PE=3 SV=1

+189

Accession Score Description
1 LPXC_AZOVD 36 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=lpxC PE=3 SV=1

+190

Accession Score Description
1 IDH_ECOLI 36 Isocitrate dehydrogenase [NADP] OS=Escherichia coli (strain K12) GN=icd PE=1 SV=1
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