MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 311 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 306 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 280 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 280 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 245 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEP1 | 221 | Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SECB_PSEPG | 208 | Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 17.1 | SECB_PSEPG |
208 | 17842 | 6 (4) | 5 (3) | 0.64 | |
| Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1 | |||||||
| 2 samesets of SECB_PSEPG | |||||||
| SECB_PSEPK |
208 | 17800 | 6 (4) | 5 (3) | 0.64 | ||
| Protein-export protein secB OS=Pseudomonas putida (strain KT2440) GN=secB PE=3 SV=1 | |||||||
| SECB_PSEP1 |
208 | 17800 | 6 (4) | 5 (3) | 0.64 | ||
| Protein-export protein secB OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=secB PE=3 SV=1 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 492 |
374.2126 | 746.4106 | 746.4075 | 4.20 | 0 | 31 | 0.57 | 2Score > 41 indicates identity |
U | K.SPQIFR.Q | |
| 864 |
453.7344 | 905.4542 | 905.4494 | 5.32 | 1 | 20 | 0.48 | 1Score > 42 indicates identity Score > 29 indicates homology |
R.DLSFEAPK.S | ||
| 2377 |
801.4161 | 1600.8176 | 1600.8097 | 4.98 | 0 | 62 | 2.1e-005 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | R.QTWEPSVALDLNTK.Q | |
| 3575 |
760.6819 | 2279.0239 | 2278.9938 | 13.2 | 1 | 93 | 3.8e-008 | 1Score > 37 indicates identity Score > 31 indicates homology |
U | M.TDQQTNGAAAEDNSPQFSLQR.I + 2 Deamidated (NQ) | |
| 3576 |
1140.5197 | 2279.0248 | 2278.9938 | 13.6 | 1 | 111 | 2.9e-010 | 1Score > 37 indicates identity Score > 28 indicates homology |
U | M.TDQQTNGAAAEDNSPQFSLQR.I + 2 Deamidated (NQ) | |
| 3946 |
862.4282 | 2584.2628 | 2584.2454 | 6.73 | 1 | 17 | 0.026 | 1Score > 38 indicates identity Score > 14 indicates homology |
U | R.GSFPALMLSPVNFDALYAQEMQR.M |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SECB_PSEPW |
59 | 17879 | 17.1 | ||
| Protein-export protein secB OS=Pseudomonas putida (strain W619) GN=secB PE=3 SV=1 | |||||
| SECB_PSEE4 |
17 | 17786 | 17.1 | ||
| Protein-export protein secB OS=Pseudomonas entomophila (strain L48) GN=secB PE=3 SV=1 | |||||
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 192 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 191 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PNP_PSEPK | 188 | Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1 |
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