MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 161–170 (out of 250)


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+161

Accession Score Description
1 SECA_HAEPS 43 Protein translocase subunit secA OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=secA PE=3 SV=1

+162

Accession Score Description
1 RL20_POLNS 43 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1

+163

Accession Score Description
1 ASTB_PSEPK 42 N-succinylarginine dihydrolase OS=Pseudomonas putida (strain KT2440) GN=astB PE=3 SV=1

+164

Accession Score Description
1 PHS_PSEAB 42 Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1

+165

Accession Score Description
1 RAVA_ECOBW 42 ATPase ravA OS=Escherichia coli (strain K12 / BW2952) GN=ravA PE=3 SV=1

+166

Accession Score Description
1 RL2_BACAA 42 50S ribosomal protein L2 OS=Bacillus anthracis (strain A0248) GN=rplB PE=3 SV=1

+167

Accession Score Description
1 COAD_HAEIE 42 Phosphopantetheine adenylyltransferase OS=Haemophilus influenzae (strain PittEE) GN=coaD PE=3 SV=1

-168

Accession Score Description
1 EFR3_CHAGB 42 Protein EFR3 OS=Chaetomium globosum GN=EFR3 PE=3 SV=1
Score Mass Matches Sequences emPAI
168.1 EFR3_CHAGB 42 130046 1 (1) 1 (1) 0.02
Protein EFR3 OS=Chaetomium globosum GN=EFR3 PE=3 SV=1
1 sameset of EFR3_CHAGB
Y2850_MYCTU 42 67117 1 (1) 1 (1) 0.03
Uncharacterized protein Rv2850c/MT2916 OS=Mycobacterium tuberculosis GN=Rv2850c PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
396   350.2306 698.4466 698.4439 3.94 0 42 0.017 +1Score > 36 indicates identity U K.LGGALLR.R

+169

Accession Score Description
1 C4AD1_DROME 41 Probable cytochrome P450 4ad1 OS=Drosophila melanogaster GN=Cyp4ad1 PE=2 SV=1

+170

Accession Score Description
1 MURB_MACCJ 41 UDP-N-acetylenolpyruvoylglucosamine reductase OS=Macrococcus caseolyticus (strain JCSC5402) GN=murB PE=3 SV=1
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