MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 311 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 306 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 280 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 280 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 245 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEP1 | 221 | Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 16.1 | SAHH_PSEP1 |
221 | 51870 | 10 (7) | 10 (7) | 0.35 | |
| Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 823 |
445.7393 | 889.4640 | 889.4545 | 10.7 | 1 | 23 | 1.3 | 7Score > 43 indicates identity Score > 36 indicates homology |
U | K.FADLPAEK.K | |
| 835 |
449.2458 | 896.4770 | 896.4716 | 6.12 | 0 | 28 | 0.23 | 1Score > 38 indicates identity Score > 35 indicates homology |
U | R.HSLNDAIK.R | |
| 841 |
449.7868 | 897.5590 | 897.5535 | 6.17 | 1 | 15 | 0.22 | 1Score > 36 indicates identity Score > 21 indicates homology |
U | R.LTVEVLPK.K | |
| 1303 |
553.8185 | 1105.6224 | 1105.6132 | 8.37 | 0 | 50 | 0.00015 | 1Score > 38 indicates identity Score > 24 indicates homology |
U | R.GFGGVVTQLTK.Q | |
| 1564 |
610.3417 | 1218.6688 | 1218.6608 | 6.57 | 0 | 63 | 0.0001 | 1Score > 40 indicates identity Score > 36 indicates homology |
U | K.QALVIGYGDVGK.G | |
| 1642 |
628.8478 | 1255.6810 | 1255.6772 | 3.05 | 0 | 61 | 3.8e-005 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | K.VPAINVNDSVTK.S | |
| 1813 |
445.9101 | 1334.7085 | 1334.7055 | 2.23 | 0 | 55 | 7.6e-005 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | R.LVNLGNATGHPSR.I | |
| 3566 |
569.7948 | 2275.1501 | 2275.1386 | 5.07 | 1 | 33 | 0.0018 | 1Score > 39 indicates identity Score > 18 indicates homology |
U | K.QQADYIGVTVEGPFKPHAYR.Y | |
| 3595 |
767.4068 | 2299.1986 | 2299.1558 | 18.6 | 1 | 34 | 0.0027 | 1Score > 38 indicates identity Score > 20 indicates homology |
U | R.IMDGSFANQVLAQIFLFEQK.F + Deamidated (NQ) | |
| 3913 |
852.0745 | 2553.2017 | 2553.1693 | 12.7 | 1 | 77 | 2.2e-007 | 1Score > 38 indicates identity Score > 23 indicates homology |
U | K.DGQPWDANMILDDGGDLTELLHK.K + Deamidated (NQ) |
15 subsets and intersections (29 subset proteins in total)17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SECB_PSEPG | 208 | Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 192 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 191 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PNP_PSEPK | 188 | Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1 |
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