MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 151–160 (out of 250)


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+151

Accession Score Description
1 RS9_PSEE4 45 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+152

Accession Score Description
1 THIO_PSEAE 45 Thioredoxin OS=Pseudomonas aeruginosa GN=trxA PE=3 SV=1

-153

Accession Score Description
1 GLNA_RHOCA 45 Glutamine synthetase (Fragment) OS=Rhodobacter capsulatus GN=glnA PE=3 SV=2
Score Mass Matches Sequences emPAI
153.1 GLNA_RHOCA 45 48702 2 (1) 2 (1) 0.05
Glutamine synthetase (Fragment) OS=Rhodobacter capsulatus GN=glnA PE=3 SV=2
1 sameset of GLNA_RHOCA
GLNA_RHOSH 45 52273 2 (1) 2 (1) 0.04
Glutamine synthetase OS=Rhodobacter sphaeroides GN=glnA PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2787   601.9862 1802.9368 1802.9666 -16.5 1 4 1.8 +4Score > 39 indicates identity
Score > 19 indicates homology
U E.LGLIFGGLTEQADNILK.Y + 2 Deamidated (NQ)
4098   912.4824 2734.4254 2734.4040 7.82 1 45 0.00015 +1Score > 36 indicates identity
Score > 19 indicates homology
U R.FPDPSANPYLAFAALLMAGLDGIKNK.I + Deamidated (NQ)

+154

Accession Score Description
1 Y1996_METMA 44 Putative ABC transporter ATP-binding protein MM_1996 OS=Methanosarcina mazei GN=MM_1996 PE=3 SV=1

+155

Accession Score Description
1 HLYB_VIBCH 44 Hemolysin secretion protein OS=Vibrio cholerae GN=hlyB PE=4 SV=2

+156

Accession Score Description
1 FABG_PSEAE 44 3-oxoacyl-[acyl-carrier-protein] reductase OS=Pseudomonas aeruginosa GN=fabG PE=3 SV=1

+157

Accession Score Description
1 DLDH1_PSEPU 44 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida GN=lpdV PE=1 SV=1

+158

Accession Score Description
1 RS5_BACAH 44 30S ribosomal protein S5 OS=Bacillus thuringiensis (strain Al Hakam) GN=rpsE PE=3 SV=1

+159

Accession Score Description
1 RRAA_PSEPG 44 Regulator of ribonuclease activity A OS=Pseudomonas putida (strain GB-1) GN=rraA PE=3 SV=1

+160

Accession Score Description
1 ATPD_PSEPG 44 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1
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