MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 250)


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+141

Accession Score Description
1 CHLB_TRIEI 47 Light-independent protochlorophyllide reductase subunit B OS=Trichodesmium erythraeum (strain IMS101) GN=chlB PE=3 SV=1

+142

Accession Score Description
1 H1L_SPISO 47 Sperm-specific protein PL-I (Fragment) OS=Spisula solidissima PE=1 SV=1

+143

Accession Score Description
1 FAK2_HUMAN 47 Protein-tyrosine kinase 2-beta OS=Homo sapiens GN=PTK2B PE=1 SV=2

+144

Accession Score Description
1 GLPD_PSETO 46 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

-145

Accession Score Description
1 MASZ_RHILV 46 Malate synthase G OS=Rhizobium leguminosarum bv. viciae GN=glcB PE=3 SV=1
Score Mass Matches Sequences emPAI
145.1 MASZ_RHILV 46 79970 2 (1) 2 (1) 0.03
Malate synthase G OS=Rhizobium leguminosarum bv. viciae GN=glcB PE=3 SV=1
+52 samesets of MASZ_RHILV

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
195   574.3115 573.3042 573.3010 5.69 0 17 0.7 +5Score > 45 indicates identity
Score > 28 indicates homology
E.AQIIE.T + Deamidated (NQ)
1691   640.8612 1279.7078 1279.6925 12.0 0 46 0.0029 +1Score > 37 indicates identity
Score > 33 indicates homology
U R.VVFINTGFLDR.T

+146

Accession Score Description
1 LPTD_BURCA 46 LPS-assembly protein lptD OS=Burkholderia cenocepacia (strain AU 1054) GN=lptD PE=3 SV=1

+147

Accession Score Description
1 SYI_MYCLE 46 Isoleucyl-tRNA synthetase OS=Mycobacterium leprae GN=ileS PE=3 SV=1

+148

Accession Score Description
1 PIFA_ECOLI 46 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

+149

Accession Score Description
1 UN84B_MOUSE 46 Protein unc-84 homolog B OS=Mus musculus GN=Unc84b PE=1 SV=2

+150

Accession Score Description
1 LPTB_PSEPU 45 Lipopolysaccharide export system ATP-binding protein lptB (Fragment) OS=Pseudomonas putida GN=lptB PE=3 SV=1
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