MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 250)


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+141

Accession Score Description
1 CHLB_TRIEI 47 Light-independent protochlorophyllide reductase subunit B OS=Trichodesmium erythraeum (strain IMS101) GN=chlB PE=3 SV=1

+142

Accession Score Description
1 H1L_SPISO 47 Sperm-specific protein PL-I (Fragment) OS=Spisula solidissima PE=1 SV=1

+143

Accession Score Description
1 FAK2_HUMAN 47 Protein-tyrosine kinase 2-beta OS=Homo sapiens GN=PTK2B PE=1 SV=2

-144

Accession Score Description
1 GLPD_PSETO 46 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1
Score Mass Matches Sequences emPAI
144.1 GLPD_PSETO 46 57294 4 (1) 2 (1) 0.04
Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1596   411.5894 1231.7464 1231.7401 5.11 0 46 0.00021 +1Score > 31 indicates identity
Score > 22 indicates homology
U R.LIQGSHLIVPR.L
3300   1067.5914 2133.1682 2133.2058 -17.6 1 0 1 +2Score > 35 indicates identity
Score > 13 indicates homology
U K.LDSPYGIRLIQGSHLIVPR.L
3304 +1 1068.0969 2134.1792 2134.1899 -4.97 1 7 1.4 +4Score > 34 indicates identity
Score > 21 indicates homology
U K.LDSPYGIRLIQGSHLIVPR.L + Deamidated (NQ)

+145

Accession Score Description
1 MASZ_RHILV 46 Malate synthase G OS=Rhizobium leguminosarum bv. viciae GN=glcB PE=3 SV=1

+146

Accession Score Description
1 LPTD_BURCA 46 LPS-assembly protein lptD OS=Burkholderia cenocepacia (strain AU 1054) GN=lptD PE=3 SV=1

+147

Accession Score Description
1 SYI_MYCLE 46 Isoleucyl-tRNA synthetase OS=Mycobacterium leprae GN=ileS PE=3 SV=1

+148

Accession Score Description
1 PIFA_ECOLI 46 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

+149

Accession Score Description
1 UN84B_MOUSE 46 Protein unc-84 homolog B OS=Mus musculus GN=Unc84b PE=1 SV=2

+150

Accession Score Description
1 LPTB_PSEPU 45 Lipopolysaccharide export system ATP-binding protein lptB (Fragment) OS=Pseudomonas putida GN=lptB PE=3 SV=1
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