MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 250)

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+11

Accession Score Description
1 DBHB_PSEAE 311 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+12

Accession Score Description
1 RPOB_PSEPG 306 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1

+13

Accession Score Description
1 EFG1_PSEPK 280 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

-14

Accession Score Description
1 DNAK_PSEPK 280 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1
Score Mass Matches Sequences emPAI
14.1 DNAK_PSEPK 280 68873 14 (8) 12 (7) 0.30
Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEPK
DNAK_PSEP1 280 65566 14 (8) 12 (7) 0.31
Chaperone protein dnaK OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=dnaK PE=2 SV=1

-14 peptide matches (13 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
436   358.2221 714.4296 714.4276 2.89 0 31 0.81 +1Score > 43 indicates identity
Score > 43 indicates homology		 R.IAGLDVK.R
599   394.7493 787.4840 787.4803 4.70 0 18 0.78 +1Score > 37 indicates identity
Score > 29 indicates homology		 U K.TQSIVIK.A
621   400.2396 798.4646 798.4599 5.89 0 26 0.092 +1Score > 38 indicates identity
Score > 28 indicates homology		 U K.VSAPVAQK.M
859   451.7711 901.5276 901.5233 4.86 1 20 0.3 +1Score > 39 indicates identity
Score > 28 indicates homology		 K.NTTIPTKK.S
921   463.7578 925.5010 925.4981 3.18 0 30 0.03 +1Score > 38 indicates identity
Score > 27 indicates homology		 U K.NDPLALQR.L
1507   399.8723 1196.5951 1196.5898 4.41 0 41 0.048 +1Score > 41 indicates identity U R.NQGDALVHSTR.K
1701   643.3646 1284.7146 1284.7038 8.46 0 76 1.3e-005 +1Score > 39 indicates identity U K.IDDVILVGGQTR.M
2228   513.9505 1538.8297 1538.8205 5.94 0 16 0.14 +1Score > 38 indicates identity
Score > 20 indicates homology		 R.QAVTNPHNTLFAVK.R
2550   839.4350 1676.8554 1676.8443 6.64 0 85 2e-006 +1Score > 41 indicates identity U R.IINEPTAAALAYGMDK.A
3296 +1 1067.5657 2133.1168 2133.0954 10.1 1 63 1.8e-006 +1Score > 38 indicates identity
Score > 19 indicates homology		 U R.TTPSIVAYANDGEILVGQSAK.R
3297   712.0500 2133.1282 2133.0954 15.4 1 25 0.005 +1Score > 37 indicates identity
Score > 15 indicates homology		 U R.TTPSIVAYANDGEILVGQSAK.R
3845   831.4342 2491.2808 2491.2330 19.2 1 73 8.2e-007 +1Score > 37 indicates identity
Score > 24 indicates homology		 U K.IELSSAQSTDVNLPYITADATGPK.H + Deamidated (NQ)
3857   835.0840 2502.2302 2502.1987 12.6 1 100 3.9e-008 +1Score > 38 indicates identity U K.SQVFSTADDNQSAVTIHVLQGER.K + Deamidated (NQ)

+14 subsets and intersections (91 subset proteins in total)

+15

Accession Score Description
1 RISB_PSEPG 245 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+16

Accession Score Description
1 SAHH_PSEP1 221 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+17

Accession Score Description
1 SECB_PSEPG 208 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 192 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+19

Accession Score Description
1 CLPB_PSEPK 191 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+20

Accession Score Description
1 PNP_PSEPK 188 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
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