MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 250)


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+131

Accession Score Description
1 SYP_PSEP1 47 Prolyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=proS PE=3 SV=1

+132

Accession Score Description
1 DNAB_MYCPN 49 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+133

Accession Score Description
1 CAPP_MYCS2 48 Phosphoenolpyruvate carboxylase OS=Mycobacterium smegmatis GN=ppc PE=1 SV=1

-134

Accession Score Description
1 LEUC_PSEF5 48 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1
Score Mass Matches Sequences emPAI
134.1 LEUC_PSEF5 48 51036 1 (1) 1 (1) 0.04
3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1

+1 peptide matches (1 non-duplicate, 0 duplicate)


+135

Accession Score Description
1 GATA_PSEPG 48 Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Pseudomonas putida (strain GB-1) GN=gatA PE=3 SV=1

+136

Accession Score Description
1 ATPE_PSEPG 48 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+137

Accession Score Description
1 TOLB_PSEPK 48 Protein tolB OS=Pseudomonas putida (strain KT2440) GN=tolB PE=3 SV=1

+138

Accession Score Description
1 RL5_BACAA 48 50S ribosomal protein L5 OS=Bacillus anthracis (strain A0248) GN=rplE PE=3 SV=1

+139

Accession Score Description
1 HFQ_PSEPK 47 Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1

+140

Accession Score Description
1 RS2_PSEP1 47 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
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