MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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131| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYP_PSEP1 | 47 | Prolyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=proS PE=3 SV=1 |
132| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAB_MYCPN | 49 | Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1 |
133| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CAPP_MYCS2 | 48 | Phosphoenolpyruvate carboxylase OS=Mycobacterium smegmatis GN=ppc PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 133.1 | CAPP_MYCS2 |
48 | 103402 | 6 (2) | 2 (1) | 0.02 | |
| Phosphoenolpyruvate carboxylase OS=Mycobacterium smegmatis GN=ppc PE=1 SV=1 | |||||||
6 peptide matches (5 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1714 |
645.8577 | 1289.7008 | 1289.7013 | -0.36 | 0 | 7 | 2.3 | 6Score > 40 indicates identity Score > 23 indicates homology |
U | R.TVLSNMAQVLAK.A + Oxidation (M) | |
| 3184 |
687.7038 | 2060.0896 | 2060.0758 | 6.69 | 1 | 16 | 1.9 | 3Score > 38 indicates identity Score > 32 indicates homology |
U | R.TVLSNMAQVLAKADMGLAAR.Y + Deamidated (NQ) | |
| 3185 |
1031.5519 | 2061.0892 | 2061.0598 | 14.3 | 1 | 6 | 4.5 | 7Score > 38 indicates identity Score > 26 indicates homology |
U | R.TVLSNMAQVLAKADMGLAAR.Y + 2 Deamidated (NQ) | |
| 3186 |
1 |
516.2812 | 2061.0957 | 2061.0598 | 17.4 | 1 | 40 | 0.0051 | 1Score > 37 indicates identity Score > 30 indicates homology |
U | R.TVLSNMAQVLAKADMGLAAR.Y + 2 Deamidated (NQ) |
| 3188 |
688.0403 | 2061.0991 | 2061.0598 | 19.1 | 1 | 8 | 0.48 | 1Score > 37 indicates identity Score > 17 indicates homology |
U | R.TVLSNMAQVLAKADMGLAAR.Y + 2 Deamidated (NQ) |
134| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LEUC_PSEF5 | 48 | 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1 |
135| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GATA_PSEPG | 48 | Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Pseudomonas putida (strain GB-1) GN=gatA PE=3 SV=1 |
136| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPE_PSEPG | 48 | ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1 |
137| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOLB_PSEPK | 48 | Protein tolB OS=Pseudomonas putida (strain KT2440) GN=tolB PE=3 SV=1 |
138| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_BACAA | 48 | 50S ribosomal protein L5 OS=Bacillus anthracis (strain A0248) GN=rplE PE=3 SV=1 |
139| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HFQ_PSEPK | 47 | Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1 |
140| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 47 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
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