MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 250)

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+11

Accession Score Description
1 DBHB_PSEAE 311 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+12

Accession Score Description
1 RPOB_PSEPG 306 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1

-13

Accession Score Description
1 EFG1_PSEPK 280 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1
Score Mass Matches Sequences emPAI
13.1 EFG1_PSEPK 280 79053 14 (10) 12 (8) 0.29
Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

-14 peptide matches (13 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
445   361.2171 720.4196 720.4170 3.64 0 15 0.47 +2Score > 38 indicates identity
Score > 24 indicates homology		 R.VVYVNK.M
608   396.7194 791.4242 791.4211 3.98 0 41 0.0028 +1Score > 40 indicates identity
Score > 28 indicates homology		 U K.IAASMATK.Q
1225   530.3007 1058.5868 1058.5794 7.04 0 55 0.002 +1Score > 40 indicates identity U R.AGDIAALIGMK.D
1229   531.7653 1061.5160 1061.5142 1.78 1 53 0.0041 +1Score > 42 indicates identity U K.LAQEDPSFR.V
1294   550.3174 1098.6202 1098.6107 8.67 0 12 0.47 +1Score > 40 indicates identity
Score > 22 indicates homology		 U K.GGGVVLEPIMK.V
1475   393.5592 1177.6558 1177.6495 5.30 0 31 0.15 +1Score > 38 indicates identity
Score > 35 indicates homology		 R.ILFYTGLSHK.M
1735   650.8914 1299.7682 1299.7551 10.1 0 18 0.037 +1Score > 34 indicates identity
Score > 16 indicates homology		 U K.NGVVAGYPLIGLK.A
1811   667.8246 1333.6346 1333.6296 3.77 1 68 2.1e-006 +1Score > 41 indicates identity
Score > 23 indicates homology		 U R.GLVQGMDESVSGR.V
2127   748.4222 1494.8298 1494.8082 14.5 0 72 1.5e-005 +1Score > 37 indicates identity U R.GITITSAAVTTFWK.G
2224 +1 768.9331 1535.8516 1535.8348 11.0 0 85 7.4e-007 +1Score > 36 indicates identity U K.IATDPFVGTLTFVR.V
2278   781.8621 1561.7096 1561.7049 3.05 0 59 1.6e-005 +1Score > 39 indicates identity
Score > 23 indicates homology		 U R.HADDDEPFSALAFK.I
2279   521.5790 1561.7152 1561.7049 6.59 0 33 0.0023 +1Score > 40 indicates identity
Score > 19 indicates homology		 U R.HADDDEPFSALAFK.I
2558   844.4415 1686.8684 1686.8539 8.65 0 27 0.28 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.MDFPEPVISLSVEPK.T

+15 subsets and intersections (119 subset proteins in total)

+14

Accession Score Description
1 DNAK_PSEPK 280 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+15

Accession Score Description
1 RISB_PSEPG 245 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+16

Accession Score Description
1 SAHH_PSEP1 221 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+17

Accession Score Description
1 SECB_PSEPG 208 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 192 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+19

Accession Score Description
1 CLPB_PSEPK 191 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+20

Accession Score Description
1 PNP_PSEPK 188 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
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