MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 311 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 306 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 12.1 | RPOB_PSEPG |
306 | 151555 | 16 (9) | 15 (9) | 0.14 | |
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 | |||||||
| 4 samesets of RPOB_PSEPG | |||||||
| RPOB_PSEPK |
306 | 151468 | 16 (9) | 15 (9) | 0.14 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1 | |||||||
| RPOB_PSEP1 |
306 | 151468 | 16 (9) | 15 (9) | 0.14 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1 | |||||||
| RPOB_PSEE4 |
306 | 151230 | 16 (9) | 15 (9) | 0.14 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1 | |||||||
| RPOB_PSEPW |
306 | 151379 | 15 (9) | 14 (9) | 0.14 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1 | |||||||
16 peptide matches (16 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 474 |
367.7332 | 733.4518 | 733.4486 | 4.38 | 0 | 11 | 0.72 | 1Score > 35 indicates identity Score > 23 indicates homology |
U | K.VYLAIR.R | |
| 805 |
441.2477 | 880.4808 | 880.4766 | 4.78 | 1 | 21 | 0.87 | 1Score > 38 indicates identity Score > 33 indicates homology |
U | R.HINQLEK.A | |
| 913 |
463.2255 | 924.4364 | 924.4413 | -5.30 | 0 | 16 | 0.82 | 1Score > 40 indicates identity Score > 28 indicates homology |
K.NIVDGDHR.M | ||
| 987 |
317.8638 | 950.5696 | 950.5661 | 3.60 | 0 | 35 | 0.042 | 1Score > 34 indicates identity |
U | R.VIVSQLHR.S | |
| 1005 |
480.2908 | 958.5670 | 958.5600 | 7.34 | 0 | 37 | 0.027 | 1Score > 37 indicates identity Score > 34 indicates homology |
U | R.GVTFAVPLR.V | |
| 1051 |
494.2661 | 986.5176 | 986.5185 | -0.89 | 1 | 13 | 1.3 | 3Score > 42 indicates identity Score > 26 indicates homology |
U | R.HLNEFTVK.A | |
| 1060 |
496.7630 | 991.5114 | 991.5087 | 2.79 | 0 | 35 | 0.23 | 1Score > 41 indicates identity |
U | K.AQQYIVDR.R | |
| 1170 |
514.3014 | 1026.5882 | 1026.5822 | 5.89 | 0 | 57 | 0.00089 | 1Score > 39 indicates identity Score > 39 indicates homology |
U | R.VSALGPGGLTR.E | |
| 1215 |
527.8196 | 1053.6246 | 1053.6182 | 6.10 | 1 | 47 | 0.0024 | 1Score > 36 indicates identity Score > 33 indicates homology |
U | K.LSLELVPQR.L | |
| 1419 |
578.3444 | 1154.6742 | 1154.6659 | 7.22 | 1 | 71 | 2.1e-005 | 1Score > 37 indicates identity |
U | K.QLIDELVAVR.H | |
| 1424 |
578.8209 | 1155.6272 | 1155.6499 | -19.6 | 1 | 7 | 5.7 | 5Score > 40 indicates identity Score > 28 indicates homology |
U | K.QLIDELVAVR.H + Deamidated (NQ) | |
| 1472 |
392.8542 | 1175.5408 | 1175.5360 | 4.06 | 0 | 34 | 0.016 | 1Score > 41 indicates identity Score > 28 indicates homology |
U | R.SPGVFFDHDR.G | |
| 2083 |
737.8533 | 1473.6920 | 1473.6888 | 2.18 | 1 | 31 | 0.092 | 1Score > 40 indicates identity Score > 33 indicates homology |
U | R.TNQYGFLESPYR.V | |
| 2428 |
811.9256 | 1621.8366 | 1621.8312 | 3.38 | 0 | 83 | 1.9e-007 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | R.STGSYSLVTQQPLGGK.A | |
| 3455 |
736.3860 | 2206.1362 | 2206.0946 | 18.8 | 1 | 52 | 1.7e-005 | 1Score > 38 indicates identity Score > 17 indicates homology |
U | K.SVFPIISYSGNAALEYVGYR.L + Deamidated (NQ) | |
| 3470 |
1111.1001 | 2220.1856 | 2220.1500 | 16.0 | 0 | 140 | 6.3e-013 | 1Score > 36 indicates identity Score > 31 indicates homology |
U | K.LPDVMDVPYLLAIQLDSYR.E |
17 subsets and intersections (296 subset proteins in total)13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 280 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 280 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 245 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEP1 | 221 | Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SECB_PSEPG | 208 | Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 192 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 191 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PNP_PSEPK | 188 | Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1 |
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