MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 111–120 (out of 250)


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+111

Accession Score Description
1 RL21_MACCJ 56 50S ribosomal protein L21 OS=Macrococcus caseolyticus (strain JCSC5402) GN=rplU PE=3 SV=1

+112

Accession Score Description
1 GRPE_PSEPK 56 Protein grpE OS=Pseudomonas putida (strain KT2440) GN=grpE PE=3 SV=1

+113

Accession Score Description
1 PYRE_PSEPG 55 Orotate phosphoribosyltransferase OS=Pseudomonas putida (strain GB-1) GN=pyrE PE=3 SV=1

+114

Accession Score Description
1 SUCC_COXBN 54 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+115

Accession Score Description
1 OPRI_PSEAE 53 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

-116

Accession Score Description
1 STU1_EMENI 53 Protein stu1 OS=Emericella nidulans GN=stu1 PE=3 SV=2
Score Mass Matches Sequences emPAI
116.1 STU1_EMENI 53 140080 1 (1) 1 (1) 0.02
Protein stu1 OS=Emericella nidulans GN=stu1 PE=3 SV=2

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
880   457.7571 913.4996 913.5093 -10.6 1 53 0.003 +1Score > 40 indicates identity U R.AGISRAPSR.A

+117

Accession Score Description
1 ARGC_AZOVD 53 N-acetyl-gamma-glutamyl-phosphate reductase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=argC PE=3 SV=1

+118

Accession Score Description
1 KPYK2_ECOLI 52 Pyruvate kinase II OS=Escherichia coli (strain K12) GN=pykA PE=1 SV=3

+119

Accession Score Description
1 RS16_PSEE4 52 30S ribosomal protein S16 OS=Pseudomonas entomophila (strain L48) GN=rpsP PE=3 SV=1

+120

Accession Score Description
1 Y1788_BACLD 52 UPF0296 protein BLi01788/BL02292 OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=BLi01788 PE=3 SV=1
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