Rita3 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita3 sp
MS data file	:	PRT1270_T-BRSC_3_20250714120051.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:41:29 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita3 sp

MS data file

PRT1270_T-BRSC_3_20250714120051.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:41:29 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	5,012

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

5,012

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (

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Protein Family Summary

Protein families 101–110 (out of 250)

Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 25 Next

101

		Accession	Score	Description
	1	SYM_PSEPG	64	Methionyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=metG PE=3 SV=1

102

		Accession	Score	Description
	1	SYS_YERPA	63	Seryl-tRNA synthetase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=serS PE=3 SV=1

103

		Accession	Score	Description
	1	SECA_PSEMY	62	Protein translocase subunit secA OS=Pseudomonas mendocina (strain ymp) GN=secA PE=3 SV=2

104

		Accession	Score	Description
	1	RPOZ_ALCBS	62	DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1

105

		Accession	Score	Description
	1	KAD_PSEE4	60	Adenylate kinase OS=Pseudomonas entomophila (strain L48) GN=adk PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
105.1	KAD_PSEE4	60	23335	6 (3)	6 (3)	0.33
	Adenylate kinase OS=Pseudomonas entomophila (strain L48) GN=adk PE=3 SV=1
	3 samesets of KAD_PSEE4
	KAD_PSEPK	60	23322	5 (3)	5 (3)	0.33
	Adenylate kinase OS=Pseudomonas putida (strain KT2440) GN=adk PE=3 SV=1
	KAD_PSEPU	60	23322	5 (3)	5 (3)	0.33
	Adenylate kinase OS=Pseudomonas putida GN=adk PE=3 SV=1
	KAD_PSEP1	60	23322	5 (3)	5 (3)	0.33
	Adenylate kinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=adk PE=3 SV=1

6 peptide matches (6 non-duplicate, 0 duplicate)

Query	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
193	573.3629	572.3556	572.3533	3.98	0	18	0.086	1Score > 43 indicates identity Score > 20 indicates homology	U	K.VLAALS.-
1078	498.3193	994.6240	994.6175	6.58	0	32	0.021	1Score > 30 indicates identity Score > 28 indicates homology	U	R.VILLGAPGAGK.G
1086	499.8005	997.5864	997.5808	5.68	1	45	0.01	1Score > 38 indicates identity	U	K.AGTPLGLELK.K
1408	576.3643	1150.7140	1150.7186	-3.97	1	3	3.6	5Score > 29 indicates identity Score > 21 indicates homology	U	M.RVILLGAPGAGK.G
2351	793.9269	1585.8392	1585.8100	18.5	1	10	4.9	5Score > 40 indicates identity Score > 29 indicates homology	U	K.LSAANAGKPKYSHIE.G + Deamidated (NQ)
3165	514.2776	2053.0813	2053.0520	14.3	0	40	0.00055	1Score > 38 indicates identity Score > 20 indicates homology	U	R.LSVYHSQTKPLVDFYQK.L + Deamidated (NQ)

3 subsets and intersections (21 subset proteins in total)

	Score	Mass	Subset of
KAD_PSEA7	48	23136	105.1
Adenylate kinase OS=Pseudomonas aeruginosa (strain PA7) GN=adk PE=3 SV=1
5 samesets of KAD_PSEA7
KAD_PSEAB	48	23150
Adenylate kinase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=adk PE=3 SV=1
KAD_PSEAE	48	23150
Adenylate kinase OS=Pseudomonas aeruginosa GN=adk PE=3 SV=1
KAD_PSEFS	48	23308
Adenylate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=adk PE=3 SV=1
KAD_PSEMY	48	23333
Adenylate kinase OS=Pseudomonas mendocina (strain ymp) GN=adk PE=3 SV=1
KAD_PSEA8	48	23150
Adenylate kinase OS=Pseudomonas aeruginosa (strain LESB58) GN=adk PE=3 SV=1
KAD_PSEPG	44	23306	105.1
Adenylate kinase OS=Pseudomonas putida (strain GB-1) GN=adk PE=3 SV=1
1 sameset of KAD_PSEPG
KAD_PSEPW	44	23306
Adenylate kinase OS=Pseudomonas putida (strain W619) GN=adk PE=3 SV=1
KAD_ALCBS	32	24010	105.1
Adenylate kinase OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=adk PE=3 SV=1
12 samesets of KAD_ALCBS
KAD_MARMS	32	23643
Adenylate kinase OS=Marinomonas sp. (strain MWYL1) GN=adk PE=3 SV=1
KAD_CELJU	32	23711
Adenylate kinase OS=Cellvibrio japonicus (strain Ueda107) GN=adk PE=3 SV=1
KAD_AZOVD	32	23393
Adenylate kinase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=adk PE=3 SV=1
KAD_METFK	32	24011
Adenylate kinase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=adk PE=3 SV=1
KAD_PSEPF	32	23231
Adenylate kinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=adk PE=3 SV=1
KAD_PSESM	32	23365
Adenylate kinase OS=Pseudomonas syringae pv. tomato GN=adk PE=3 SV=1
KAD_PSEU2	32	23360
Adenylate kinase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=adk PE=3 SV=1
KAD_PSEF5	32	23292
Adenylate kinase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=adk PE=3 SV=1
KAD_CHLP8	32	24093
Adenylate kinase OS=Chlorobaculum parvum (strain NCIB 8327) GN=adk PE=3 SV=1
KAD_PSE14	32	23533
Adenylate kinase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=adk PE=3 SV=1
KAD_HAEDU	32	23667
Adenylate kinase OS=Haemophilus ducreyi GN=adk PE=3 SV=1
KAD_NEIMU	32	23245
Adenylate kinase OS=Neisseria mucosa GN=adk PE=3 SV=1

106

		Accession	Score	Description
	1	ILVD_PSEFS	57	Dihydroxy-acid dehydratase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvD PE=3 SV=1

107

		Accession	Score	Description
	1	ODBA_PSEPU	57	2-oxoisovalerate dehydrogenase subunit alpha OS=Pseudomonas putida GN=bkdA1 PE=1 SV=2

108

		Accession	Score	Description
	1	PYRG_PSEPG	57	CTP synthase OS=Pseudomonas putida (strain GB-1) GN=pyrG PE=3 SV=1

109

		Accession	Score	Description
	1	ODO1_AZOVI	56	2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

110

		Accession	Score	Description
	1	TYPH_AZOC5	56	Putative thymidine phosphorylase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=AZC_2467 PE=3 SV=1

Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 25 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 101–110 (out of 250)

101

102

103

104

105

6 peptide matches (6 non-duplicate, 0 duplicate)

3 subsets and intersections (21 subset proteins in total)

106

107

108

109

110