MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 101–110 (out of 250)


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+101

Accession Score Description
1 SYM_PSEPG 64 Methionyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=metG PE=3 SV=1

+102

Accession Score Description
1 SYS_YERPA 63 Seryl-tRNA synthetase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=serS PE=3 SV=1

+103

Accession Score Description
1 SECA_PSEMY 62 Protein translocase subunit secA OS=Pseudomonas mendocina (strain ymp) GN=secA PE=3 SV=2

-104

Accession Score Description
1 RPOZ_ALCBS 62 DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1
Score Mass Matches Sequences emPAI
104.1 RPOZ_ALCBS 62 9584 1 (1) 1 (1) 0.26
DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1
12 samesets of RPOZ_ALCBS
RPOZ_PSEAB 62 9826 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoZ PE=3 SV=1
RPOZ_PSEAE 62 9826 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas aeruginosa GN=rpoZ PE=3 SV=1
RPOZ_PSEMY 62 9785 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas mendocina (strain ymp) GN=rpoZ PE=3 SV=1
RPOZ_PSEPG 62 9797 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas putida (strain GB-1) GN=rpoZ PE=3 SV=1
RPOZ_PSEPK 62 9797 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas putida (strain KT2440) GN=rpoZ PE=3 SV=1
RPOZ_PSEPW 62 9770 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas putida (strain W619) GN=rpoZ PE=3 SV=1
RPOZ_PSEP1 62 9797 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoZ PE=3 SV=1
RPOZ_PSEE4 62 9780 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas entomophila (strain L48) GN=rpoZ PE=3 SV=1
RPOZ_PSEF5 62 9727 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoZ PE=3 SV=2
RPOZ_PSEU5 62 9860 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas stutzeri (strain A1501) GN=rpoZ PE=3 SV=1
RPOZ_PSEA7 62 9826 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas aeruginosa (strain PA7) GN=rpoZ PE=3 SV=1
RPOZ_PSEA8 62 9826 1 (1) 1 (1) 0.25
DNA-directed RNA polymerase subunit omega OS=Pseudomonas aeruginosa (strain LESB58) GN=rpoZ PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2369   533.2990 1596.8752 1596.8624 8.01 1 62 2.2e-005 +1Score > 38 indicates identity
Score > 28 indicates homology
U R.VAWENDKPTVVALR.E

+105

Accession Score Description
1 KAD_PSEE4 60 Adenylate kinase OS=Pseudomonas entomophila (strain L48) GN=adk PE=3 SV=1

+106

Accession Score Description
1 ILVD_PSEFS 57 Dihydroxy-acid dehydratase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvD PE=3 SV=1

+107

Accession Score Description
1 ODBA_PSEPU 57 2-oxoisovalerate dehydrogenase subunit alpha OS=Pseudomonas putida GN=bkdA1 PE=1 SV=2

+108

Accession Score Description
1 PYRG_PSEPG 57 CTP synthase OS=Pseudomonas putida (strain GB-1) GN=pyrG PE=3 SV=1

+109

Accession Score Description
1 ODO1_AZOVI 56 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+110

Accession Score Description
1 TYPH_AZOC5 56 Putative thymidine phosphorylase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=AZC_2467 PE=3 SV=1
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