MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1177 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 5 | EFTU_CARRP | 73 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1045 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
| 3 | EFTU_BACAA | 271 | Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1 | |
| 4 | EFTU_ANATD | 83 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 525 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 5 | ATPG_RHOP5 | 40 | description | |
| 3 | ATPB_LEGPA | 167 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
| 2 | CH60_PSEPK | 473 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 6 | CH602_PROMS | 39 | 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1 | |
| 4 | CH602_SINMW | 59 | 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPG | 522 | ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 112 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 463 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 391 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPG | 350 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1 | |
| 2 | RPOC_PSEA7 | 244 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1 | |
| 3 | FTSZ_PSEPK | 95 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 349 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 327 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 321 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 313 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 10.1 | SUCC_PSEPK |
313 | 41499 | 20 (15) | 13 (10) | 0.91 | |
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 | |||||||
| 1 sameset of SUCC_PSEPK | |||||||
| SUCC_PSEP1 |
313 | 41499 | 20 (15) | 13 (10) | 0.91 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sucC PE=3 SV=1 | |||||||
20 peptide matches (16 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 331 |
334.7275 | 667.4404 | 667.4381 | 3.50 | 0 | 31 | 0.031 | 1Score > 28 indicates identity |
U | K.VPVVVR.L | |
| 493 |
374.2434 | 746.4722 | 746.4691 | 4.28 | 0 | 32 | 0.072 | 1Score > 33 indicates identity |
K.IFVGLAK.L | ||
| 726 |
848.4649 | 847.4576 | 847.4552 | 2.86 | 0 | 36 | 0.097 | 1Score > 41 indicates identity Score > 38 indicates homology |
U | K.QVQQFAK.I | |
| 727 |
424.7368 | 847.4590 | 847.4552 | 4.54 | 0 | 35 | 0.22 | 1Score > 41 indicates identity |
U | K.QVQQFAK.I | |
| 1059 |
1 |
496.2741 | 990.5336 | 990.5287 | 5.01 | 0 | 36 | 0.0062 | 1Score > 41 indicates identity Score > 27 indicates homology |
U | K.AFAAQWLGK.N |
| 1092 |
501.2781 | 1000.5416 | 1000.5342 | 7.46 | 1 | 27 | 0.053 | 1Score > 42 indicates identity Score > 27 indicates homology |
U | K.IGGNEWVVK.A | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.rawScore > 40 indicates identity Score > 27 indicates homology |
|||||||||||
| 1366 |
567.8171 | 1133.6196 | 1133.6081 | 10.2 | 1 | 26 | 0.062 | 1Score > 40 indicates identity Score > 27 indicates homology |
K.ELYLGAVVDR.S | ||
| 19.5 | 1 | 12 | 1.4 | 2 | VSGASITMRGR | ||||||
| 10.2 | 1 | 12 | 1.5 | 3 | QIYIAAGELR + Deamidated (NQ) | ||||||
| -11.9 | 1 | 11 | 1.9 | 4 | IEIYINQIK + Deamidated (NQ) | ||||||
| -11.9 | 1 | 9 | 2.9 | 5 | QLYLIKNLE + Deamidated (NQ) | ||||||
| 16.0 | 0 | 9 | 3.5 | 6 | MHHLIDQLK | ||||||
| 10.2 | 0 | 8 | 3.8 | 7 | IYQIDGGIVR + Deamidated (NQ) | ||||||
| 10.2 | 1 | 8 | 4 | 8 | EIIDYLVNR | ||||||
| 0.33 | 0 | 7 | 4.7 | 9 | QLYNQLSIR | ||||||
| -11.9 | 0 | 7 | 5 | 10 | QLYQLIVQK + 2 Deamidated (NQ) | ||||||
| 1477 |
590.7950 | 1179.5754 | 1179.5706 | 4.11 | 0 | 50 | 0.0071 | 1Score > 41 indicates identity |
U | K.INIDANAMYR.Q | |
| 1708 |
644.7839 | 1287.5532 | 1287.5480 | 4.07 | 0 | 32 | 0.054 | 1Score > 37 indicates identity Score > 32 indicates homology |
U | K.TFHDPSQDDAR.E | |
| 1709 |
430.1917 | 1287.5533 | 1287.5480 | 4.09 | 0 | 39 | 0.0025 | 1Score > 37 indicates identity Score > 25 indicates homology |
U | K.TFHDPSQDDAR.E | |
| 1840 |
1 |
676.3760 | 1350.7374 | 1350.7183 | 14.1 | 1 | 43 | 0.0006 | 1Score > 38 indicates identity Score > 24 indicates homology |
U | K.QLFAEYGLPVSK.G |
| 2290 |
523.9544 | 1568.8414 | 1568.8311 | 6.55 | 0 | 54 | 4.2e-005 | 1Score > 38 indicates identity Score > 23 indicates homology |
U | K.ATIDPLVGAQPFQGR.E | |
| 2291 |
1 |
785.4283 | 1568.8420 | 1568.8311 | 6.98 | 0 | 79 | 5.7e-007 | 1Score > 38 indicates identity Score > 29 indicates homology |
U | K.ATIDPLVGAQPFQGR.E |
| 2562 |
563.6633 | 1687.9681 | 1687.9509 | 10.2 | 1 | 55 | 2.1e-005 | 1Score > 33 indicates identity Score > 21 indicates homology |
U | K.DHDLALLEVNPLVIK.A | |
| 2662 |
868.4415 | 1734.8684 | 1734.8424 | 15.0 | 0 | 71 | 1.9e-006 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | K.NLVTYQTDANGQPVSK.I + Deamidated (NQ) | |
| 3755 |
1 |
805.1266 | 2412.3580 | 2412.3111 | 19.4 | 1 | 66 | 1.9e-005 | 1Score > 31 indicates identity |
U | K.VLAESGLNIIAATSLTDAAQQVVK.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SUCC_PSEE4 |
313 | 41472 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas entomophila (strain L48) GN=sucC PE=3 SV=1 | |||||
| 1 sameset of SUCC_PSEE4 | |||||
| SUCC_PSEPW |
313 | 41472 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain W619) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEPG |
220 | 41413 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain GB-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEPF |
215 | 41527 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf0-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSE14 |
208 | 41494 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sucC PE=3 SV=1 | |||||
| 4 samesets of SUCC_PSE14 | |||||
| SUCC_PSEFS |
208 | 41547 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEF5 |
208 | 41599 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSESM |
208 | 41472 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. tomato GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEU2 |
208 | 41442 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEA7 |
147 | 41772 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain PA7) GN=sucC PE=3 SV=1 | |||||
| 3 samesets of SUCC_PSEA7 | |||||
| SUCC_PSEAB |
147 | 41830 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEAE |
147 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa GN=sucC PE=3 SV=2 | |||||
| SUCC_PSEA8 |
147 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain LESB58) GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBC3 |
71 | 41658 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_VIBC3 | |||||
| SUCC_VIBCH |
71 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBCM |
71 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain M66-2) GN=sucC PE=3 SV=1 | |||||
| SUCC_MARAV |
55 | 41495 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_MARAV | |||||
| SUCC_PSEU5 |
55 | 41761 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=sucC PE=3 SV=1 | |||||
| SUCC_SACD2 |
55 | 41524 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEMY |
51 | 41759 | 10.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas mendocina (strain ymp) GN=sucC PE=3 SV=1 | |||||
| 1 sameset of SUCC_PSEMY | |||||
| SUCC_TERTT |
51 | 41703 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=sucC PE=3 SV=1 | |||||
| PARB2_DEIRA |
31 | 32872 | 10.1 | ||
| Probable chromosome 2-partitioning protein parB OS=Deinococcus radiodurans GN=parB2 PE=3 SV=1 | |||||
| 46 samesets of PARB2_DEIRA |
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