MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 250)

Page: 1 2 3 4 5 6 … 25 Next 

-1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1177 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_CARRP 73 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1045 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 EFTU_BACAA 271 Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
4 EFTU_ANATD 83 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
1.1 EFTU2_PSEPK 1177 43793 74 (55) 19 (16) 4.97
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1177 43793 74 (55) 19 (16) 4.97
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
1.2 EFTU1_PSEPK 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
1.3 EFTU_BACAA 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
14 samesets of EFTU_BACAA
EFTU_BACAC 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) GN=tuf PE=3 SV=1
EFTU_BACAH 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus thuringiensis (strain Al Hakam) GN=tuf PE=3 SV=1
EFTU_BACAN 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus anthracis GN=tuf PE=3 SV=1
EFTU_BACC0 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain AH820) GN=tuf PE=3 SV=1
EFTU_BACCQ 271 43012 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain Q1) GN=tuf PE=3 SV=1
EFTU_BACCR 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=tuf PE=3 SV=1
EFTU_BACCZ 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain ZK / E33L) GN=tuf PE=3 SV=1
EFTU_BACHK 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus thuringiensis subsp. konkukian GN=tuf PE=3 SV=1
EFTU_BACWK 271 43241 17 (13) 7 (6) 0.59
Elongation factor Tu OS=Bacillus weihenstephanensis (strain KBAB4) GN=tuf PE=3 SV=1
EFTU_BACC1 271 43012 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain ATCC 10987) GN=tuf PE=3 SV=1
EFTU_BACC2 271 43084 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain G9842) GN=tuf PE=3 SV=1
EFTU_BACC3 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain 03BB102) GN=tuf PE=3 SV=1
EFTU_BACC4 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain B4264) GN=tuf PE=3 SV=1
EFTU_BACC7 271 43012 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain AH187) GN=tuf PE=3 SV=1
1.4 EFTU_ANATD 83 44208 11 (4) 4 (3) 0.22
Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
1 sameset of EFTU_ANATD
EFTU_CALS8 83 44218 12 (4) 5 (3) 0.22
Elongation factor Tu OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=tuf PE=3 SV=1
1.5 EFTU_CARRP 73 44439 10 (4) 2 (2) 0.16
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

+94 peptide matches (59 non-duplicate, 35 duplicate)

+66 subsets and intersections (640 subset proteins in total)

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 525 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
5 ATPG_RHOP5 40 description
3 ATPB_LEGPA 167 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
2 CH60_PSEPK 473 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
6 CH602_PROMS 39 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1
4 CH602_SINMW 59 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPG 522 ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 ATPA_RICAH 112 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1

+4

Accession Score Description
1 ARCA_PSEPK 463 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+5

Accession Score Description
1 OTCC_PSEPK 391 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+6

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 350 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
2 RPOC_PSEA7 244 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 FTSZ_PSEPK 95 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+7

Accession Score Description
1 DLDH2_PSEPU 349 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+8

Accession Score Description
1 ADHP_ECOLI 327 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+9

Accession Score Description
1 ACON2_PSEAE 321 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+10

Accession Score Description
1 SUCC_PSEPK 313 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
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