MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 250)

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-1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1177 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_CARRP 73 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1045 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 EFTU_BACAA 271 Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
4 EFTU_ANATD 83 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 1177 43793 74 (55) 19 (16) 4.97
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1177 43793 74 (55) 19 (16) 4.97
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
EFTU1_PSEPK 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1045 43810 71 (52) 19 (16) 4.12
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
EFTU_BACAA 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
14 samesets of EFTU_BACAA
EFTU_BACAC 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) GN=tuf PE=3 SV=1
EFTU_BACAH 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus thuringiensis (strain Al Hakam) GN=tuf PE=3 SV=1
EFTU_BACAN 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus anthracis GN=tuf PE=3 SV=1
EFTU_BACC0 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain AH820) GN=tuf PE=3 SV=1
EFTU_BACCQ 271 43012 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain Q1) GN=tuf PE=3 SV=1
EFTU_BACCR 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=tuf PE=3 SV=1
EFTU_BACCZ 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain ZK / E33L) GN=tuf PE=3 SV=1
EFTU_BACHK 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus thuringiensis subsp. konkukian GN=tuf PE=3 SV=1
EFTU_BACWK 271 43241 17 (13) 7 (6) 0.59
Elongation factor Tu OS=Bacillus weihenstephanensis (strain KBAB4) GN=tuf PE=3 SV=1
EFTU_BACC1 271 43012 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain ATCC 10987) GN=tuf PE=3 SV=1
EFTU_BACC2 271 43084 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain G9842) GN=tuf PE=3 SV=1
EFTU_BACC3 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain 03BB102) GN=tuf PE=3 SV=1
EFTU_BACC4 271 43026 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain B4264) GN=tuf PE=3 SV=1
EFTU_BACC7 271 43012 17 (13) 7 (6) 0.60
Elongation factor Tu OS=Bacillus cereus (strain AH187) GN=tuf PE=3 SV=1
EFTU_ANATD 83 44208 11 (4) 4 (3) 0.22
Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
1 sameset of EFTU_ANATD
EFTU_CALS8 83 44218 12 (4) 5 (3) 0.22
Elongation factor Tu OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=tuf PE=3 SV=1
EFTU_CARRP 73 44439 10 (4) 2 (2) 0.16
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

-94 peptide matches (59 non-duplicate, 35 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 Peptide
240   615.3862 614.3789 614.3752 6.14 0 19 3.7 +7Score > 42 indicates identity
Score > 37 indicates homology		 X R.GQVLAK.S
378 +1 345.2033 688.3920 688.3868 7.61 0 32 0.33 +1Score > 41 indicates identity
Score > 40 indicates homology		 X X X R.GTVVTGR  
381 +1 689.4001 688.3928 688.3868 8.74 0 35 0.11 +1Score > 41 indicates identity
Score > 38 indicates homology		 X X X R.GTVVTGR  
407   351.6941 701.3736 701.3708 4.08 1 24 0.49 +1Score > 43 indicates identity
Score > 33 indicates homology		 X X K.LLDEGR.A
569 +3 388.7098 775.4050 775.4017 4.29 0 18 0.35 +1Score > 42 indicates identity
Score > 26 indicates homology		 U X X X R.HTPFFK.G
571   776.4124 775.4051 775.4017 4.39 0 32 0.023 +1Score > 42 indicates identity
Score > 28 indicates homology		 U X X X R.HTPFFK.G
626 +1 401.2470 800.4794 800.4756 4.79 0 42 0.0034 +1Score > 42 indicates identity
Score > 29 indicates homology		 U X X R.TVGAGVVAK.I
629 +1 801.4910 800.4837 800.4756 10.1 0 49 0.0074 +1Score > 40 indicates identity U X X R.TVGAGVVAK.I
768   867.5078 866.5005 866.4974 3.63 1 54 0.00097 +1Score > 37 indicates identity U X X X R.EHILLSR.Q
769 +1 434.2579 866.5012 866.4974 4.47 1 40 0.025 +1Score > 36 indicates identity U X X X R.EHILLSR.Q
973   947.5555 946.5482 946.5447 3.68 0 63 0.00015 +1Score > 38 indicates identity U X X K.TTLTAALTR.V
976 +2 474.2823 946.5500 946.5447 5.61 0 81 8.9e-007 +1Score > 37 indicates identity
Score > 33 indicates homology		 U X X K.TTLTAALTR.V
1101   1005.5073 1004.5000 1004.4961 3.94 1 60 0.00065 +1Score > 42 indicates identity
Score > 40 indicates homology		 U X X K.TIAMEDGLR.F
1102 +1 503.2575 1004.5004 1004.4961 4.37 1 52 0.0014 +1Score > 42 indicates identity
Score > 36 indicates homology		 U X X K.TIAMEDGLR.F
1273   544.7825 1087.5504 1087.5444 5.54 1 69 1.7e-005 +1Score > 42 indicates identity
Score > 34 indicates homology		 U X X R.AGENCGVLLR.G
1421 +3 578.8196 1155.6246 1155.6176 6.13 1 47 0.0029 +1Score > 40 indicates identity
Score > 34 indicates homology		 U X X K.FTAEVYVLSK.E
1422   1156.6322 1155.6249 1155.6176 6.36 1 63 0.00023 +1Score > 40 indicates identity U X X K.FTAEVYVLSK.E
1597   617.3143 1232.6140 1232.6091 4.04 0 22 0.14 +1Score > 42 indicates identity
Score > 26 indicates homology		 U X X K.GYRPQFYFR.T
1599 +2 411.8791 1232.6155 1232.6091 5.19 0 31 0.016 +1Score > 42 indicates identity
Score > 26 indicates homology		 U X X K.GYRPQFYFR.T
1610   413.5778 1237.7116 1237.7030 6.90 1 65 6.6e-005 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
1613 +3 619.8654 1237.7162 1237.7030 10.7 1 69 2.6e-005 +1Score > 36 indicates identity U X X R.VQDPLEIVGLR.D
1743   652.4053 1302.7960 1302.7759 15.5 0 52 1.9e-005 +1Score > 28 indicates identity
Score > 17 indicates homology		 U X K.TTLTAAITTVLAK.A
2090 +2 492.6300 1474.8682 1474.8548 9.07 0 55 0.00027 +1Score > 32 indicates identity U X X X R.QVGVPYIVVFLNK  
2093 +2 738.4445 1474.8744 1474.8548 13.3 0 57 0.00014 +1Score > 31 indicates identity U X X X R.QVGVPYIVVFLNK  
2218   766.9551 1531.8956 1531.8763 12.7 0 59 1e-005 +1Score > 32 indicates identity
Score > 21 indicates homology		 U X R.QVNVPYIVVFLNK.V
2310   315.7944 1573.9356 1573.9304 3.30 0 3 1 +1Score > 30 indicates identity
Score > 15 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2311   787.9755 1573.9364 1573.9304 3.83 0 66 1.3e-005 +1Score > 30 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2311   787.9755 1573.9364 1573.9304 3.84 0 40 0.0046 +2Score > 30 indicates identity U X R.GQVLAKPGTIKPHTK.F
2314 +1 525.6532 1573.9378 1573.9304 4.67 0 54 0.00017 +1Score > 29 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2314 +1 525.6532 1573.9378 1573.9304 4.68 0 14 1.8 +2Score > 29 indicates identity U X R.GQVLAKPGTIKPHTK.F
2315   394.4920 1573.9389 1573.9304 5.39 0 33 0.0018 +1Score > 29 indicates identity
Score > 18 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2315   394.4920 1573.9389 1573.9304 5.39 0 3 1.6 +2Score > 29 indicates identity
Score > 18 indicates homology		 U X R.GQVLAKPGTIKPHTK.F
2319 +1 315.9951 1574.9391 1574.9144 15.7 0 22 0.025 +1Score > 29 indicates identity
Score > 19 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2412 +1 807.9497 1613.8848 1613.8665 11.4 1 68 2.3e-006 +1Score > 38 indicates identity
Score > 24 indicates homology		 U X K.LVETLDAYIPEPVR.A
2440 +1 815.9467 1629.8788 1629.8614 10.7 1 75 5.6e-006 +1Score > 39 indicates identity
Score > 35 indicates homology		 U X K.LVETLDSYIPEPVR.A
2517   834.4664 1666.9182 1666.9002 10.8 0 59 5.2e-006 +1Score > 37 indicates identity
Score > 19 indicates homology		 U X K.LLDQAQAGDNIGALLR.G
2522   835.4322 1668.8498 1668.8682 -11.0 0 1 1.4 +5Score > 40 indicates identity
Score > 15 indicates homology		 U X K.LLDQAQAGDNIGALLR.G + 2 Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.raw

Score > 40 indicates identity

Score > 22 indicates homology
-10.6 1 10 0.99 1 LLDEAQAGDNIGALLR   + Deamidated (NQ)
2523   557.2908 1668.8506 1668.8682 -10.6 0 10 0.99 -1Score > 40 indicates identity
Score > 22 indicates homology		 U X K.LLDQAQAGDNIGALLR.G + 2 Deamidated (NQ)
0.037 0 9 1.1 3 TPSDAVVVSHQLMLR   + Deamidated (NQ); Oxidation (M)
-17.3 0 9 1.1 4 GSLISVIDHTVTASGGR  
-8.99 1 8 1.6 5 GRSQQPSQGQSQLLR  
11.2 1 7 1.9 6 IEGTNHLSGNVAISGAK   + 2 Deamidated (NQ)
-10.6 1 7 2.1 7 LLDEGQAGDNVGVLLR   + Deamidated (NQ)
-10.6 1 7 2.1 7 LLDQGEAGDNVGVLLR   + Deamidated (NQ)
-10.6 0 7 2.1 7 LLDQGQAGDNVGVLLR   + 2 Deamidated (NQ)
-2.26 1 6 2.4 10 TRVPQSPSQNQGTIR   + Deamidated (NQ)
2723   883.9711 1765.9276 1765.9224 2.98 0 86 9.4e-007 +1Score > 39 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2725 +1 354.1932 1765.9296 1765.9224 4.09 0 33 0.056 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T
2726 +1 442.4897 1765.9297 1765.9224 4.13 0 49 0.0054 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2728   354.3938 1766.9326 1766.9064 14.8 0 31 0.037 +1Score > 39 indicates identity
Score > 30 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2729   442.7406 1766.9333 1766.9064 15.2 0 20 0.078 +1Score > 39 indicates identity
Score > 22 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2730   589.9857 1766.9353 1766.9064 16.3 0 52 7.1e-005 +1Score > 38 indicates identity
Score > 23 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2733   590.2689 1767.7849 1767.7787 3.46 0 35 0.0065 +1Score > 39 indicates identity
Score > 26 indicates homology		 U X X X X R.HYAHVDCPGHADYVK.N
2734   442.9539 1767.7865 1767.7787 4.38 0 20 0.035 +1Score > 39 indicates identity
Score > 18 indicates homology		 U X X X X R.HYAHVDCPGHADYVK.N
2760   596.9897 1787.9473 1787.9166 17.2 1 19 0.059 +1Score > 39 indicates identity
Score > 19 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2762   597.3161 1788.9265 1788.9006 14.5 1 57 1.5e-005 +1Score > 40 indicates identity
Score > 21 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2775   601.3138 1800.9196 1800.9118 4.30 1 64 0.00023 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2776   901.4673 1800.9200 1800.9118 4.56 1 86 1.3e-006 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2779 +1 601.6479 1801.9219 1801.8958 14.4 1 63 0.00027 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
2780   901.9686 1801.9226 1801.8958 14.9 1 81 4.2e-006 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
2896   939.4854 1876.9562 1876.9458 5.54 1 47 8e-005 +1Score > 40 indicates identity
Score > 18 indicates homology		 U X R.DLLSEYGFPGDDIPVIK.G
3303   712.3770 2134.1092 2134.0769 15.1 1 34 0.11 +1Score > 38 indicates identity
Score > 37 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G
3309 +1 1068.5590 2135.1034 2135.0609 19.9 1 72 5.4e-006 +1Score > 38 indicates identity
Score > 32 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3342   1076.5542 2151.0938 2151.0558 17.7 1 71 3.4e-007 +1Score > 39 indicates identity
Score > 19 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ); Oxidation (M)
3424 +2 731.7148 2192.1226 2192.1551 -14.8 1 44 0.00046 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3426 +1 1097.0692 2192.1238 2192.1551 -14.3 1 34 0.026 +1Score > 38 indicates identity
Score > 30 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3545   1133.5791 2265.1436 2265.1165 12.0 0 17 0.16 +1Score > 39 indicates identity
Score > 22 indicates homology		 U X X R.DLLSTYDFPGDDTPIIIGSAR.M

+66 subsets and intersections (640 subset proteins in total)

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 525 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
5 ATPG_RHOP5 40 description
3 ATPB_LEGPA 167 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
2 CH60_PSEPK 473 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
6 CH602_PROMS 39 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1
4 CH602_SINMW 59 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPG 522 ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 ATPA_RICAH 112 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1

+4

Accession Score Description
1 ARCA_PSEPK 463 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+5

Accession Score Description
1 OTCC_PSEPK 391 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+6

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 350 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
2 RPOC_PSEA7 244 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 FTSZ_PSEPK 95 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+7

Accession Score Description
1 DLDH2_PSEPU 349 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+8

Accession Score Description
1 ADHP_ECOLI 327 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+9

Accession Score Description
1 ACON2_PSEAE 321 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+10

Accession Score Description
1 SUCC_PSEPK 313 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
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