MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1781 414.2470 1239.7192 1239.7339 -11.9 1 15 0.35 +1Score > 36 indicates identity
Score > 23 indicates homology
HPSKLLAYALK
2509 533.6160 1597.8262 1597.8311 -3.08 1 15 0.38 +1Score > 40 indicates identity
Score > 23 indicates homology
QSVNLKNDPLALQR + 3 Deamidated (NQ)
2821 871.9955 1741.9764 1741.9826 -3.51 1 15 0.6 +1Score > 35 indicates identity
Score > 25 indicates homology
KLVLDNDVDTAVSLLK
3021 933.5015 1864.9884 1864.9570 16.8 1 15 0.5 +1Score > 38 indicates identity
Score > 24 indicates homology
FTEPQAEAIVSLQLYR + Deamidated (NQ)
412 333.1873 664.3600 664.3544 8.48 1 15 0.13 +1Score > 39 indicates identity
Score > 18 indicates homology
VEVYR
457 675.3821 674.3748 674.3711 5.49 1 15 0.79 +1Score > 40 indicates identity
Score > 26 indicates homology
DISKGR
2180 479.2730 1434.7972 1434.7943 2.03 1 15 0.97 +1Score > 37 indicates identity
Score > 27 indicates homology
SPNRPPQIQKIR + 2 Deamidated (NQ)
1077 470.7920 939.5694 939.5640 5.75 1 15 0.78 +1Score > 35 indicates identity
Score > 26 indicates homology
NPILLLEK + Deamidated (NQ)
843 426.2130 850.4114 850.4120 -0.60 0 15 0.98 +1Score > 42 indicates identity
Score > 27 indicates homology
ICFQQR
2096 699.8877 1397.7608 1397.7514 6.76 1 15 0.81 +1Score > 39 indicates identity
Score > 26 indicates homology
VLALLNDNLQRE + Deamidated (NQ)
1209 495.2821 988.5496 988.5593 -9.77 0 15 0.31 +1Score > 40 indicates identity
Score > 22 indicates homology
ILGFNISPK + Deamidated (NQ)
2036 457.5850 1369.7332 1369.7453 -8.82 0 15 0.49 +1Score > 40 indicates identity
Score > 24 indicates homology
AANILVNDQGILK + 2 Deamidated (NQ)
2308 502.5971 1504.7695 1504.7643 3.47 1 15 0.55 +1Score > 41 indicates identity
Score > 25 indicates homology
WALQAMGKMAGGIR + Oxidation (M)
2687 835.5066 1668.9986 1668.9661 19.5 1 15 0.62 +1Score > 29 indicates identity
Score > 25 indicates homology
LNIILQGNIILSTEK + Deamidated (NQ)
739 401.2505 800.4864 800.4909 -5.51 0 15 0.73 +1Score > 40 indicates identity
Score > 26 indicates homology
GIVLPFR
2944 906.9580 1811.9014 1811.9265 -13.8 0 15 0.82 +1Score > 40 indicates identity
Score > 26 indicates homology
NNVVIIDTDNPSQILR + 2 Deamidated (NQ)
308 602.3607 601.3534 601.3547 -2.18 1 15 0.91 +1Score > 45 indicates identity
Score > 27 indicates homology
KDAIR
1038 464.2737 926.5328 926.5297 3.36 1 15 0.82 +1Score > 38 indicates identity
Score > 26 indicates homology
RNIPTGLR + Deamidated (NQ)
2084 695.8788 1389.7430 1389.7220 15.1 1 15 0.73 +1Score > 40 indicates identity
Score > 26 indicates homology
QMVAMRLLAQGR + Deamidated (NQ); Oxidation (M)
1718 610.8378 1219.6610 1219.6561 4.08 0 15 0.97 +1Score > 40 indicates identity
Score > 27 indicates homology
ISLGLFSNVNR + Deamidated (NQ)
2098 700.3309 1398.6472 1398.6514 -2.98 0 15 0.99 +1Score > 40 indicates identity
Score > 27 indicates homology
IIHQITDLNNSE + 3 Deamidated (NQ)
2891 895.4592 1788.9038 1788.9298 -14.5 0 14 0.98 +1Score > 40 indicates identity
Score > 27 indicates homology
ISFDLNVAQGLALLWE + Deamidated (NQ)
3961 509.6463 2543.1951 2543.2447 -19.5 1 14 0.42 +1Score > 38 indicates identity
Score > 23 indicates homology
GALCSIACGASLFCAADVYRIIR
524 352.7221 703.4296 703.4228 9.68 1 14 0.75 +1Score > 35 indicates identity
Score > 26 indicates homology
TKQTVK
2948 909.4717 1816.9288 1816.9353 -3.53 1 14 0.062 +1Score > 40 indicates identity
Score > 15 indicates homology
MLQNITRNLITQDQK + 2 Deamidated (NQ)
3042 938.9552 1875.8958 1875.8950 0.47 1 14 0.41 +1Score > 40 indicates identity
Score > 23 indicates homology
QIEVTTDGAGVSGTVVDVE + Deamidated (NQ)
855 427.7424 853.4702 853.4657 5.29 0 14 0.19 +1Score > 37 indicates identity
Score > 20 indicates homology
APAAAPAASK
1614 391.8615 1172.5627 1172.5648 -1.83 1 14 0.43 +1Score > 40 indicates identity
Score > 23 indicates homology
HFVEMEPIR + Oxidation (M)
917 440.2536 878.4926 878.4895 3.56 1 14 0.98 +1Score > 37 indicates identity
Score > 27 indicates homology
AKMSIVSK + Oxidation (M)
213 489.2796 488.2723 488.2707 3.31 0 14 0.49 +1Score > 44 indicates identity
Score > 24 indicates homology
TVGGR
1508 564.8255 1127.6364 1127.6438 -6.48 1 14 0.94 +1Score > 40 indicates identity
Score > 27 indicates homology
INLIDAVLEK + Deamidated (NQ)
3398 1059.0406 2116.0666 2116.0840 -8.22 1 14 0.98 +1Score > 39 indicates identity
Score > 27 indicates homology
QYLNDLGIPFEIDPNLVR + Deamidated (NQ)
2810 578.5978 1732.7716 1732.7951 -13.6 1 14 0.24 +1Score > 38 indicates identity
Score > 21 indicates homology
HNQSPRNTYQSAMAK + Deamidated (NQ)
1309 343.5317 1027.5733 1027.5774 -4.04 1 14 0.79 +1Score > 40 indicates identity
Score > 26 indicates homology
RVADAVAAQK
3307 1030.5331 2059.0516 2059.0884 -17.9 1 14 0.9 +1Score > 39 indicates identity
Score > 26 indicates homology
ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
572 364.7253 727.4360 727.4453 -12.7 1 14 0.91 +1Score > 39 indicates identity
Score > 27 indicates homology
LVGAGRR
1695 606.3568 1210.6990 1210.6809 15.0 1 14 0.4 +1Score > 36 indicates identity
Score > 23 indicates homology
VIVPEAITIQE
2003 677.8826 1353.7506 1353.7728 -16.4 1 14 0.46 +1Score > 38 indicates identity
Score > 24 indicates homology
IVRSILDGATGPR
3768 1175.5758 2349.1370 2349.1812 -18.8 1 14 0.19 +1Score > 39 indicates identity
Score > 20 indicates homology
TNPNFNQSVLNTALKTNSSGIK + 2 Deamidated (NQ)
1521 566.8272 1131.6398 1131.6322 6.76 0 14 0.43 +1Score > 38 indicates identity
Score > 23 indicates homology
VQGMVLTTGVK
1919 660.3527 1318.6908 1318.6881 2.09 0 14 0.73 +1Score > 41 indicates identity
Score > 25 indicates homology
QLIQQNGLTFR + 2 Deamidated (NQ)
285 574.3315 573.3242 573.3234 1.37 0 14 0.5 +1Score > 41 indicates identity
Score > 24 indicates homology
AGSIAR
796 417.2208 832.4270 832.4225 5.43 1 14 0.98 +1Score > 43 indicates identity
Score > 27 indicates homology
GKGCVGGAK
913 440.2528 878.4910 878.4974 -7.20 1 14 0.83 +1Score > 38 indicates identity
Score > 26 indicates homology
AKAHQVPK + Deamidated (NQ)
2632 825.9311 1649.8476 1649.8624 -8.97 0 14 0.26 +1Score > 40 indicates identity
Score > 21 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
1466 554.2519 1106.4892 1106.4914 -1.91 1 14 0.75 +1Score > 40 indicates identity
Score > 26 indicates homology
VLDQMNENK + Deamidated (NQ); Oxidation (M)
2916 901.9128 1801.8110 1801.8094 0.94 1 14 0.76 +1Score > 39 indicates identity
Score > 26 indicates homology
NGSSAKWMGPFYIGDR + Deamidated (NQ); Oxidation (M)
1881 649.3486 1296.6826 1296.6898 -5.53 1 14 0.1 +1Score > 40 indicates identity
Score > 17 indicates homology
NVTPNNNIRQK
1891 653.8411 1305.6676 1305.6565 8.55 1 14 0.64 +1Score > 42 indicates identity
Score > 25 indicates homology
LFSEQGNVVDAK
2034 684.3524 1366.6902 1366.6803 7.31 1 14 0.84 +1Score > 40 indicates identity
Score > 26 indicates homology
TSGFEMVQGLLGK + Deamidated (NQ)
691 390.2289 778.4432 778.4337 12.2 1 14 0.81 +1Score > 37 indicates identity
Score > 26 indicates homology
FGAKATGK
1666 399.8914 1196.6524 1196.6401 10.3 0 14 0.73 +1Score > 38 indicates identity
Score > 25 indicates homology
EPLDSLLSPAR
2978 460.7478 1838.9621 1838.9924 -16.5 0 14 0.52 +1Score > 38 indicates identity
Score > 24 indicates homology
TSTIGLGMPTAPAAVLAPR + Oxidation (M)
1707 608.3593 1214.7040 1214.6870 14.0 1 14 0.65 +1Score > 37 indicates identity
Score > 25 indicates homology
QVNKVTAAVGVK + 2 Deamidated (NQ)
323 307.7092 613.4038 613.4024 2.43 1 14 0.55 +1Score > 37 indicates identity
Score > 24 indicates homology
RVAIR
939 446.7443 891.4740 891.4702 4.35 1 14 0.72 +1Score > 41 indicates identity
Score > 25 indicates homology
VAEFIADK
2278 748.4574 1494.9002 1494.8770 15.6 0 14 0.11 +1Score > 30 indicates identity
Score > 17 indicates homology
LAADVTGVPTLLVAR
1162 485.7657 969.5168 969.5284 -11.9 0 14 0.92 +1Score > 39 indicates identity
Score > 26 indicates homology
DVPNVWIK
2284 500.2941 1497.8605 1497.8667 -4.18 1 14 0.17 +1Score > 35 indicates identity
Score > 19 indicates homology
RVFQAAQALGIPVK + Deamidated (NQ)
1582 1159.7120 1158.7047 1158.7046 0.12 1 14 0.2 +1Score > 31 indicates identity
Score > 20 indicates homology
SLPLLMITKK + Oxidation (M)
1642 593.3394 1184.6642 1184.6513 10.9 1 14 0.34 +1Score > 39 indicates identity
Score > 22 indicates homology
VQAAIQGDKVR + Deamidated (NQ)
2782 574.3103 1719.9091 1719.9380 -16.8 1 14 0.78 +1Score > 39 indicates identity
Score > 26 indicates homology
DTQRTALPHINSIVR
1387 356.8539 1067.5399 1067.5247 14.2 1 14 0.7 +1Score > 40 indicates identity
Score > 25 indicates homology
LDNATYKSR + Deamidated (NQ)
462 340.7040 679.3934 679.3904 4.42 1 14 0.67 +1Score > 35 indicates identity
Score > 25 indicates homology
YKQIK + Deamidated (NQ)
1501 376.2017 1125.5833 1125.5852 -1.72 1 14 0.54 +1Score > 39 indicates identity
Score > 24 indicates homology
KIQASSMAFK + Oxidation (M)
1506 564.8080 1127.6014 1127.5896 10.5 1 14 0.29 +1Score > 41 indicates identity
Score > 21 indicates homology
LVEIQMPAVE
721 398.1989 794.3832 794.3810 2.83 1 14 0.72 +1Score > 41 indicates identity
Score > 25 indicates homology
ENYVAAK + Deamidated (NQ)
1685 602.8383 1203.6620 1203.6459 13.4 1 14 0.25 +1Score > 39 indicates identity
Score > 20 indicates homology
SVSTARQLTLE
1709 609.3057 1216.5968 1216.6200 -19.1 0 14 0.71 +1Score > 42 indicates identity
Score > 25 indicates homology
TAQVWLSQQR + Deamidated (NQ)
1291 508.7697 1015.5248 1015.5186 6.17 0 14 0.72 +1Score > 42 indicates identity
Score > 25 indicates homology
LADGIDIDGK
2290 500.5887 1498.7443 1498.7667 -15.0 0 14 0.97 +1Score > 40 indicates identity
Score > 26 indicates homology
GQLAQINVKPNWK + 4 Deamidated (NQ)
2348 759.9399 1517.8652 1517.8705 -3.44 1 14 0.48 +1Score > 35 indicates identity
Score > 23 indicates homology
ISLVNIKYLVLDE
3037 936.4672 1870.9198 1870.9061 7.36 1 14 0.54 +1Score > 40 indicates identity
Score > 24 indicates homology
AAVHELSDYLIGQDPSR + Deamidated (NQ)
3308 1030.5332 2059.0518 2059.0545 -1.30 1 14 0.33 +1Score > 39 indicates identity
Score > 22 indicates homology
IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
2400 517.2734 1548.7984 1548.7817 10.7 1 14 0.44 +1Score > 40 indicates identity
Score > 23 indicates homology
ETGMINQSLLTLGR + Deamidated (NQ); Oxidation (M)
2153 712.3625 1422.7104 1422.7368 -18.5 1 14 0.59 +1Score > 41 indicates identity
Score > 24 indicates homology
IQIGHFDRAHTK + Deamidated (NQ)
565 364.2179 726.4212 726.4276 -8.74 0 14 0.44 +1Score > 36 indicates identity
Score > 23 indicates homology
GPVLVDK
2743 851.9234 1701.8322 1701.8210 6.64 0 14 0.63 +1Score > 40 indicates identity
Score > 24 indicates homology
LTNTNINQLDQWIE + Deamidated (NQ)
1738 410.2280 1227.6622 1227.6823 -16.4 1 14 0.93 +1Score > 40 indicates identity
Score > 26 indicates homology
QPDSQVTVKVK
341 309.6960 617.3774 617.3748 4.24 0 14 0.51 +1Score > 38 indicates identity
Score > 24 indicates homology
TAVVTK
1156 323.5234 967.5484 967.5491 -0.72 1 14 0.72 +1Score > 36 indicates identity
Score > 25 indicates homology
KAYFQLAK
3420 1064.5638 2127.1130 2127.0749 17.9 1 14 0.79 +1Score > 38 indicates identity
Score > 25 indicates homology
IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
291 586.3881 585.3808 585.3737 12.1 0 14 0.69 +1Score > 39 indicates identity
Score > 25 indicates homology
ILDIL
2193 722.4128 1442.8110 1442.7841 18.7 1 14 0.2 +1Score > 37 indicates identity
Score > 20 indicates homology
GNQAIQRSIDTIK
643 381.2080 760.4014 760.3967 6.31 1 14 0.99 +1Score > 44 indicates identity
Score > 26 indicates homology
TAEQIAK + Deamidated (NQ)
3070 947.5030 1892.9914 1893.0030 -6.08 0 14 0.87 +1Score > 39 indicates identity
Score > 26 indicates homology
IAITTRPHQIVMPSAIE + Deamidated (NQ); Oxidation (M)
2417 519.3021 1554.8845 1554.8558 18.4 1 14 0.66 +1Score > 36 indicates identity
Score > 25 indicates homology
RFPVALALSAPVWE
2697 837.4747 1672.9348 1672.9070 16.7 1 14 0.59 +1Score > 36 indicates identity
Score > 24 indicates homology
EKPTILLMVGVQGSGK + Deamidated (NQ); Oxidation (M)
2988 922.9943 1843.9740 1843.9415 17.7 0 14 0.29 +1Score > 39 indicates identity
Score > 21 indicates homology
TANGATIDQIAQLIDGLK + 3 Deamidated (NQ)
3559 550.2764 2197.0765 2197.0666 4.50 0 14 0.94 +1Score > 39 indicates identity
Score > 26 indicates homology
HNFSVWNMLLYFNSALPK + Deamidated (NQ); Oxidation (M)
356 314.6992 627.3838 627.3956 -18.7 0 14 0.42 +1Score > 33 indicates identity
Score > 23 indicates homology
AVTIPK
1827 637.8120 1273.6094 1273.6037 4.49 1 14 0.9 +1Score > 41 indicates identity
Score > 26 indicates homology
DEINDALINQK + 2 Deamidated (NQ)
3187 663.6533 1987.9381 1987.9673 -14.7 1 14 0.83 +1Score > 39 indicates identity
Score > 25 indicates homology
QSLNQFMAVYKLNDSSK + Oxidation (M)
1273 506.3136 1010.6126 1010.6012 11.4 0 14 0.7 +1Score > 33 indicates identity
Score > 25 indicates homology
INALQLIPK + 2 Deamidated (NQ)
1765 413.2123 1236.6151 1236.6237 -6.98 1 14 0.54 +1Score > 41 indicates identity
Score > 24 indicates homology
KNNYIIINNK + 4 Deamidated (NQ)
2427 520.6233 1558.8481 1558.8454 1.74 0 14 0.36 +1Score > 38 indicates identity
Score > 22 indicates homology
LANLSISSTAQVLLE + Deamidated (NQ)
2418 519.3026 1554.8860 1554.8729 8.39 1 14 0.79 +1Score > 36 indicates identity
Score > 25 indicates homology
GDSRVPAISAASIIAK
3184 663.3197 1986.9373 1986.9534 -8.12 1 14 0.28 +1Score > 39 indicates identity
Score > 21 indicates homology
FSHINQPVKTLSGGNQQK + 5 Deamidated (NQ)
1332 519.2743 1036.5340 1036.5488 -14.2 1 14 0.62 +1Score > 40 indicates identity
Score > 24 indicates homology
LAAAFKMNR + Oxidation (M)
3289 1023.0355 2044.0564 2044.0312 12.3 1 14 0.86 +1Score > 39 indicates identity
Score > 26 indicates homology
RYAQLGWVMQLHIGAQR + 2 Deamidated (NQ); Oxidation (M)
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