MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2902 598.9807 1793.9203 1793.9498 -16.5 0 16 0.41 +1Score > 39 indicates identity
Score > 25 indicates homology
GNAPMLPVWLSPIQVR + Deamidated (NQ); Oxidation (M)
395 652.3063 651.2990 651.3010 -3.03 0 16 0.82 +1Score > 41 indicates identity
Score > 28 indicates homology
MATSAR + Oxidation (M)
545 358.7016 715.3886 715.3864 3.10 0 16 0.5 +1Score > 44 indicates identity
Score > 25 indicates homology
QDAAALK
1303 343.1932 1026.5578 1026.5498 7.73 1 16 0.18 +1Score > 39 indicates identity
Score > 21 indicates homology
RVYDTFVK
1734 410.2274 1227.6604 1227.6823 -17.9 1 16 0.65 +1Score > 40 indicates identity
Score > 27 indicates homology
QPDSQVTVKVK
606 372.7360 743.4574 743.4541 4.48 1 16 0.96 +1Score > 41 indicates identity
Score > 28 indicates homology
KLDGAIK
3421 710.0463 2127.1171 2127.0960 9.90 1 16 0.56 +1Score > 38 indicates identity
Score > 26 indicates homology
AADTAGDLAGLEIAGPGFINVR
3818 797.4378 2389.2916 2389.3104 -7.88 1 16 0.11 +1Score > 35 indicates identity
Score > 19 indicates homology
LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ)
2529 805.3820 1608.7494 1608.7600 -6.54 1 16 0.38 +1Score > 40 indicates identity
Score > 24 indicates homology
KMGMNIINDDITGR + 2 Oxidation (M)
3199 667.0001 1997.9785 1997.9656 6.46 0 16 0.16 +1Score > 40 indicates identity
Score > 20 indicates homology
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
1478 372.5355 1114.5847 1114.5982 -12.2 1 16 0.58 +1Score > 41 indicates identity
Score > 26 indicates homology
GADKDAAALVGK
2702 839.4270 1676.8394 1676.8477 -4.93 0 16 0.66 +1Score > 41 indicates identity
Score > 27 indicates homology
AITQSLTPSMVIMPR + Deamidated (NQ); 2 Oxidation (M)
2207 485.9591 1454.8555 1454.8318 16.3 1 16 0.23 +1Score > 33 indicates identity
Score > 22 indicates homology
TAVVVGAGRSGLAAAR
2932 904.4625 1806.9104 1806.9220 -6.37 0 16 0.44 +1Score > 40 indicates identity
Score > 25 indicates homology
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M)
2033 684.3524 1366.6902 1366.6803 7.31 1 16 0.76 +1Score > 40 indicates identity
Score > 27 indicates homology
TSGFEMVQGLLGK + Deamidated (NQ)
4268 976.5329 2926.5769 2926.6254 -16.6 1 16 1 +1Score > 33 indicates identity
Score > 28 indicates homology
ARIAGVNIPNHQHTVIGLTAIFGIGTTR
2516 801.4422 1600.8698 1600.8573 7.84 0 16 0.15 +1Score > 38 indicates identity
Score > 20 indicates homology
SVAATDIWQQLLTR
1057 465.7686 929.5226 929.5083 15.5 1 16 0.87 +1Score > 42 indicates identity
Score > 28 indicates homology
LGAAWQKR + Deamidated (NQ)
2933 904.4626 1806.9106 1806.9220 -6.26 0 16 0.31 +1Score > 40 indicates identity
Score > 23 indicates homology
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M)
1777 620.3268 1238.6390 1238.6394 -0.31 0 16 0.45 +1Score > 40 indicates identity
Score > 25 indicates homology
VTASLPTPVQPE + Deamidated (NQ)
592 368.7280 735.4414 735.4391 3.13 0 16 0.8 +1Score > 34 indicates identity
Score > 27 indicates homology
LPHTIR
731 400.7179 799.4212 799.4188 3.05 1 16 0.66 +1Score > 39 indicates identity
Score > 26 indicates homology
VDPQGKR + Deamidated (NQ)
1086 314.8370 941.4892 941.4930 -4.11 0 16 0.77 +1Score > 40 indicates identity
Score > 27 indicates homology
VPSGQLADR
1843 639.8381 1277.6616 1277.6438 14.0 0 16 0.99 +1Score > 41 indicates identity
Score > 28 indicates homology
LLDIYPMQGGR + Oxidation (M)
2929 602.6409 1804.9009 1804.8964 2.48 1 16 0.29 +1Score > 40 indicates identity
Score > 23 indicates homology
RLSCMPGIANFLQVAE
2393 774.3964 1546.7782 1546.7641 9.18 1 16 0.82 +1Score > 41 indicates identity
Score > 27 indicates homology
YQPQVGFNRNAPR + Deamidated (NQ)
3039 938.4541 1874.8936 1874.9110 -9.24 1 16 0.75 +1Score > 40 indicates identity
Score > 27 indicates homology
QIEVTTDGAGVSGTVVDVE
2254 738.9022 1475.7898 1475.7620 18.9 0 16 0.69 +1Score > 40 indicates identity
Score > 27 indicates homology
ANNAILQFLDISR + 2 Deamidated (NQ)
2151 712.3610 1422.7074 1422.7354 -19.7 1 16 0.58 +1Score > 41 indicates identity
Score > 26 indicates homology
LQSQIQSKFQSK + 2 Deamidated (NQ)
277 569.3069 568.2996 568.2969 4.78 0 16 0.32 +1Score > 38 indicates identity
Score > 23 indicates homology
DVHAK
1767 413.5601 1237.6585 1237.6666 -6.59 1 16 0.51 +1Score > 39 indicates identity
Score > 25 indicates homology
ENLDGLIHISK
2380 769.9156 1537.8166 1537.8212 -2.99 0 16 0.64 +1Score > 39 indicates identity
Score > 26 indicates homology
TTLPSQGPLQRPSR + Deamidated (NQ)
3334 693.7088 2078.1046 2078.0983 3.03 0 16 0.61 +1Score > 37 indicates identity
Score > 26 indicates homology
LNSAVFPGGQGGPLMHIIAAK + Deamidated (NQ)
1794 417.9066 1250.6980 1250.6758 17.7 1 16 0.49 +1Score > 37 indicates identity
Score > 25 indicates homology
SSLLPKLTTYE
1902 438.5667 1312.6783 1312.6623 12.2 0 16 0.63 +1Score > 40 indicates identity
Score > 26 indicates homology
VEPGLGADNSVVR + Deamidated (NQ)
633 380.2542 758.4938 758.4902 4.85 0 16 0.7 +1Score > 30 indicates identity
Score > 27 indicates homology
VISSLIK
2280 748.8718 1495.7290 1495.7129 10.8 0 16 0.36 +1Score > 41 indicates identity
Score > 24 indicates homology
FMIAANGFEPDLR + Oxidation (M)
2281 748.8997 1495.7848 1495.8021 -11.5 1 16 0.9 +1Score > 40 indicates identity
Score > 28 indicates homology
IPIELLQVNDIVE + 2 Deamidated (NQ)
879 430.7487 859.4828 859.4837 -1.00 0 16 0.88 +1Score > 41 indicates identity
Score > 27 indicates homology
MLDAAVIK
1584 580.3617 1158.7088 1158.7046 3.67 1 16 0.086 +1Score > 31 indicates identity
Score > 17 indicates homology
SLPLLMITKK + Oxidation (M)
2904 897.9713 1793.9280 1793.9523 -13.5 1 16 0.33 +1Score > 39 indicates identity
Score > 23 indicates homology
QQDIPVENVLALGGIAR + 2 Deamidated (NQ)
2504 799.4194 1596.8242 1596.7968 17.2 0 16 0.68 +1Score > 40 indicates identity
Score > 26 indicates homology
QGQQQQQLQQQQK
1744 410.5762 1228.7068 1228.7139 -5.81 1 16 0.83 +1Score > 38 indicates identity
Score > 27 indicates homology
QISSLGQQIKK
2656 555.2968 1662.8686 1662.8537 8.97 1 16 0.27 +1Score > 40 indicates identity
Score > 22 indicates homology
ISGASGLASNTTNTKNK
3333 693.7086 2078.1040 2078.0983 2.75 0 15 0.14 +1Score > 37 indicates identity
Score > 19 indicates homology
LNSAVFPGGQGGPLMHIIAAK + Deamidated (NQ)
1366 528.2869 1054.5592 1054.5407 17.6 1 15 0.66 +1Score > 39 indicates identity
Score > 26 indicates homology
NSIETQVHK
960 450.2890 898.5634 898.5600 3.87 0 15 0.7 +1Score > 37 indicates identity
Score > 26 indicates homology
ANLLSILR
3228 1006.5301 2011.0456 2011.0473 -0.85 1 15 0.66 +1Score > 39 indicates identity
Score > 26 indicates homology
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ)
1365 352.4958 1054.4656 1054.4866 -19.9 0 15 0.45 +1Score > 39 indicates identity
Score > 24 indicates homology
GAFLSNGSMR + Oxidation (M)
1824 636.8414 1271.6682 1271.6479 16.0 0 15 0.45 +1Score > 41 indicates identity
Score > 24 indicates homology
GLLHCMVTSVR
2045 458.8860 1373.6362 1373.6575 -15.5 0 15 0.12 +1Score > 40 indicates identity
Score > 19 indicates homology
TSIEPDGSLWNR
1328 518.2980 1034.5814 1034.6012 -19.1 1 15 0.37 +1Score > 38 indicates identity
Score > 24 indicates homology
IEIYIVGTK
1126 319.5147 955.5223 955.5312 -9.30 1 15 0.64 +1Score > 39 indicates identity
Score > 26 indicates homology
GRGNAQVVR
1603 391.2047 1170.5923 1170.5782 12.0 1 15 0.64 +1Score > 40 indicates identity
Score > 26 indicates homology
FHGVADQELR
1715 610.3054 1218.5962 1218.5728 19.3 1 15 0.84 +1Score > 42 indicates identity
Score > 27 indicates homology
NTAEVAQGAASAK + 2 Deamidated (NQ)
1934 442.2681 1323.7825 1323.7809 1.20 1 15 0.43 +1Score > 33 indicates identity
Score > 24 indicates homology
GVIAMLARQLPR
2753 570.2929 1707.8569 1707.8389 10.5 0 15 0.92 +1Score > 40 indicates identity
Score > 27 indicates homology
TSFQMLPISNEPISK + Deamidated (NQ); Oxidation (M)
2931 904.4619 1806.9092 1806.9363 -15.0 1 15 0.52 +1Score > 40 indicates identity
Score > 25 indicates homology
QAGVPIVPGSQGIIENVE + Deamidated (NQ)
1816 633.8837 1265.7528 1265.7343 14.6 0 15 0.65 +1Score > 33 indicates identity
Score > 26 indicates homology
LQLQQLGLVPR + 2 Deamidated (NQ)
1119 476.2492 950.4838 950.4709 13.6 1 15 0.85 +1Score > 41 indicates identity
Score > 27 indicates homology
AGEIVYDGK
825 423.2246 844.4346 844.4290 6.67 1 15 0.77 +1Score > 43 indicates identity
Score > 27 indicates homology
NVANIGEK + Deamidated (NQ)
1703 304.4160 1213.6349 1213.6489 -11.5 1 15 0.37 +1Score > 40 indicates identity
Score > 23 indicates homology
MINPNLVLER + Oxidation (M)
1157 323.8391 968.4955 968.5039 -8.74 0 15 0.69 +1Score > 39 indicates identity
Score > 26 indicates homology
GSTGAPPNLR
2209 728.4357 1454.8568 1454.8569 -0.059 1 15 0.62 +1Score > 33 indicates identity
Score > 26 indicates homology
VSVTVLGVKGNQVR
2334 379.2166 1512.8373 1512.8512 -9.16 0 15 0.5 +1Score > 38 indicates identity
Score > 25 indicates homology
TSGDLLAGLLLDGLR
3227 671.3558 2011.0456 2011.0262 9.63 1 15 0.47 +1Score > 39 indicates identity
Score > 24 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
3436 1068.5627 2135.1108 2135.0858 11.7 1 15 0.15 +1Score > 38 indicates identity
Score > 19 indicates homology
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
1640 593.3312 1184.6478 1184.6448 2.59 1 15 0.84 +1Score > 40 indicates identity
Score > 27 indicates homology
NCQALRAKPK
2238 736.8672 1471.7198 1471.7381 -12.4 0 15 0.33 +1Score > 40 indicates identity
Score > 23 indicates homology
WALMIDPQGQALK + 2 Deamidated (NQ)
292 586.3884 585.3811 585.3737 12.6 0 15 0.23 +1Score > 39 indicates identity
Score > 21 indicates homology
ILDIL
635 380.2561 758.4976 758.4902 9.86 0 15 0.8 +1Score > 28 indicates identity
Score > 27 indicates homology
SVLSILK
335 308.6877 615.3608 615.3592 2.74 0 15 0.9 +1Score > 45 indicates identity
Score > 27 indicates homology
IVDAAK
2030 456.5702 1366.6888 1366.6915 -1.98 0 15 0.74 +1Score > 40 indicates identity
Score > 26 indicates homology
GTYVCIATNALGK
2292 750.4482 1498.8818 1498.8541 18.5 1 15 0.18 +1Score > 32 indicates identity
Score > 20 indicates homology
NKLLIIDGMNLVR + Deamidated (NQ)
1314 1031.5144 1030.5071 1030.5196 -12.1 0 15 0.71 +1Score > 41 indicates identity
Score > 26 indicates homology
NGVWSIAAGR + Deamidated (NQ)
2298 752.3866 1502.7586 1502.7576 0.68 1 15 0.48 +1Score > 41 indicates identity
Score > 24 indicates homology
TSLTLADIQDAEAR
3226 671.3558 2011.0456 2011.0262 9.63 1 15 0.46 +1Score > 39 indicates identity
Score > 24 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
2023 683.8416 1365.6686 1365.6646 2.99 1 15 0.27 +1Score > 41 indicates identity
Score > 22 indicates homology
GRHTVPCMPPAK + Oxidation (M)
436 336.7014 671.3882 671.3966 -12.4 1 15 0.69 +1Score > 42 indicates identity
Score > 26 indicates homology
KGAATPK
852 854.4759 853.4686 853.4657 3.37 1 15 0.97 +1Score > 38 indicates identity
Score > 27 indicates homology
EHLVASAK
3385 704.3737 2110.0993 2110.0921 3.40 1 15 0.28 +1Score > 38 indicates identity
Score > 22 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
722 398.1991 794.3836 794.3810 3.32 1 15 0.57 +1Score > 41 indicates identity
Score > 25 indicates homology
AVFENSK + Deamidated (NQ)
2634 825.9319 1649.8492 1649.8777 -17.2 0 15 0.71 +1Score > 40 indicates identity
Score > 26 indicates homology
AIQNSFFLLINDVR + Deamidated (NQ)
2497 532.2680 1593.7822 1593.7787 2.17 0 15 0.76 +1Score > 41 indicates identity
Score > 26 indicates homology
DAYAVTPFNTPTAAR
4004 862.4822 2584.4248 2584.3999 9.61 1 15 1 +1Score > 32 indicates identity
Score > 27 indicates homology
SKGSATALYSQLLDTTLQILYLGK + Deamidated (NQ)
920 440.7268 879.4390 879.4338 5.98 0 15 0.78 +1Score > 42 indicates identity
Score > 26 indicates homology
DAGVDIYK
2035 684.3527 1366.6908 1366.6803 7.75 1 15 0.8 +1Score > 40 indicates identity
Score > 26 indicates homology
TSGFEMVQGLLGK + Deamidated (NQ)
3714 577.0298 2304.0901 2304.1096 -8.49 1 15 0.79 +1Score > 39 indicates identity
Score > 26 indicates homology
VVTDKAPTDDQWDAMLLGWK + Oxidation (M)
1210 990.5140 989.5067 989.5215 -15.0 1 15 0.4 +1Score > 42 indicates identity
Score > 23 indicates homology
KVNLGMSLE
1949 665.3514 1328.6882 1328.6936 -4.02 1 15 0.3 +1Score > 41 indicates identity
Score > 22 indicates homology
QVVVNLLGSRGGE + 2 Deamidated (NQ)
2317 755.9507 1509.8868 1509.8654 14.2 0 15 0.17 +1Score > 32 indicates identity
Score > 20 indicates homology
LIVLTDTPGILLNE
1118 475.7536 949.4926 949.4869 6.09 1 15 0.76 +1Score > 42 indicates identity
Score > 26 indicates homology
LDAYEALR
1607 586.8334 1171.6522 1171.6448 6.34 1 15 0.47 +1Score > 40 indicates identity
Score > 24 indicates homology
NLSAAVLDELK
3259 1017.0264 2032.0382 2032.0364 0.91 0 15 0.16 +1Score > 39 indicates identity
Score > 19 indicates homology
ILIHINNTNPILDLDSPE + 2 Deamidated (NQ)
2361 510.6254 1528.8544 1528.8402 9.27 0 15 0.29 +1Score > 37 indicates identity
Score > 22 indicates homology
AFNIDLGFPLIGPR
2369 766.9457 1531.8768 1531.8511 16.8 1 15 0.63 +1Score > 34 indicates identity
Score > 25 indicates homology
SRVGIVSWGAYIPK
3054 471.7317 1882.8977 1882.8818 8.44 0 15 0.52 +1Score > 40 indicates identity
Score > 24 indicates homology
LCSLNINMHHDPFLR + Deamidated (NQ); Oxidation (M)
2538 538.2720 1611.7942 1611.7933 0.57 0 15 0.35 +1Score > 40 indicates identity
Score > 23 indicates homology
ANFLAISGYIWNNK + 2 Deamidated (NQ)
219 501.3167 500.3094 500.3071 4.68 0 15 0.23 +1Score > 43 indicates identity
Score > 21 indicates homology
AVVGR
747 403.7245 805.4344 805.4334 1.31 0 15 0.8 +1Score > 41 indicates identity
Score > 26 indicates homology
LFNDLGK
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