| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 598.9807 | 1793.9203 | 1793.9498 | -16.5 | 0 | 16 | 0.41 | 1Score > 39 indicates identityScore > 25 indicates homology |
GNAPMLPVWLSPIQVR + Deamidated (NQ); Oxidation (M) | |
| 652.3063 | 651.2990 | 651.3010 | -3.03 | 0 | 16 | 0.82 | 1Score > 41 indicates identityScore > 28 indicates homology |
MATSAR + Oxidation (M) | |
| 358.7016 | 715.3886 | 715.3864 | 3.10 | 0 | 16 | 0.5 | 1Score > 44 indicates identityScore > 25 indicates homology |
QDAAALK | |
| 343.1932 | 1026.5578 | 1026.5498 | 7.73 | 1 | 16 | 0.18 | 1Score > 39 indicates identityScore > 21 indicates homology |
RVYDTFVK | |
| 410.2274 | 1227.6604 | 1227.6823 | -17.9 | 1 | 16 | 0.65 | 1Score > 40 indicates identityScore > 27 indicates homology |
QPDSQVTVKVK | |
| 372.7360 | 743.4574 | 743.4541 | 4.48 | 1 | 16 | 0.96 | 1Score > 41 indicates identityScore > 28 indicates homology |
KLDGAIK | |
| 710.0463 | 2127.1171 | 2127.0960 | 9.90 | 1 | 16 | 0.56 | 1Score > 38 indicates identityScore > 26 indicates homology |
AADTAGDLAGLEIAGPGFINVR | |
| 797.4378 | 2389.2916 | 2389.3104 | -7.88 | 1 | 16 | 0.11 | 1Score > 35 indicates identityScore > 19 indicates homology |
LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ) | |
| 805.3820 | 1608.7494 | 1608.7600 | -6.54 | 1 | 16 | 0.38 | 1Score > 40 indicates identityScore > 24 indicates homology |
KMGMNIINDDITGR + 2 Oxidation (M) | |
| 667.0001 | 1997.9785 | 1997.9656 | 6.46 | 0 | 16 | 0.16 | 1Score > 40 indicates identityScore > 20 indicates homology |
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M) | |
| 372.5355 | 1114.5847 | 1114.5982 | -12.2 | 1 | 16 | 0.58 | 1Score > 41 indicates identityScore > 26 indicates homology |
GADKDAAALVGK | |
| 839.4270 | 1676.8394 | 1676.8477 | -4.93 | 0 | 16 | 0.66 | 1Score > 41 indicates identityScore > 27 indicates homology |
AITQSLTPSMVIMPR + Deamidated (NQ); 2 Oxidation (M) | |
| 485.9591 | 1454.8555 | 1454.8318 | 16.3 | 1 | 16 | 0.23 | 1Score > 33 indicates identityScore > 22 indicates homology |
TAVVVGAGRSGLAAAR | |
| 904.4625 | 1806.9104 | 1806.9220 | -6.37 | 0 | 16 | 0.44 | 1Score > 40 indicates identityScore > 25 indicates homology |
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M) | |
| 684.3524 | 1366.6902 | 1366.6803 | 7.31 | 1 | 16 | 0.76 | 1Score > 40 indicates identityScore > 27 indicates homology |
TSGFEMVQGLLGK + Deamidated (NQ) | |
| 976.5329 | 2926.5769 | 2926.6254 | -16.6 | 1 | 16 | 1 | 1Score > 33 indicates identityScore > 28 indicates homology |
ARIAGVNIPNHQHTVIGLTAIFGIGTTR | |
| 801.4422 | 1600.8698 | 1600.8573 | 7.84 | 0 | 16 | 0.15 | 1Score > 38 indicates identityScore > 20 indicates homology |
SVAATDIWQQLLTR | |
| 465.7686 | 929.5226 | 929.5083 | 15.5 | 1 | 16 | 0.87 | 1Score > 42 indicates identityScore > 28 indicates homology |
LGAAWQKR + Deamidated (NQ) | |
| 904.4626 | 1806.9106 | 1806.9220 | -6.26 | 0 | 16 | 0.31 | 1Score > 40 indicates identityScore > 23 indicates homology |
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M) | |
| 620.3268 | 1238.6390 | 1238.6394 | -0.31 | 0 | 16 | 0.45 | 1Score > 40 indicates identityScore > 25 indicates homology |
VTASLPTPVQPE + Deamidated (NQ) | |
| 368.7280 | 735.4414 | 735.4391 | 3.13 | 0 | 16 | 0.8 | 1Score > 34 indicates identityScore > 27 indicates homology |
LPHTIR | |
| 400.7179 | 799.4212 | 799.4188 | 3.05 | 1 | 16 | 0.66 | 1Score > 39 indicates identityScore > 26 indicates homology |
VDPQGKR + Deamidated (NQ) | |
| 314.8370 | 941.4892 | 941.4930 | -4.11 | 0 | 16 | 0.77 | 1Score > 40 indicates identityScore > 27 indicates homology |
VPSGQLADR | |
| 639.8381 | 1277.6616 | 1277.6438 | 14.0 | 0 | 16 | 0.99 | 1Score > 41 indicates identityScore > 28 indicates homology |
LLDIYPMQGGR + Oxidation (M) | |
| 602.6409 | 1804.9009 | 1804.8964 | 2.48 | 1 | 16 | 0.29 | 1Score > 40 indicates identityScore > 23 indicates homology |
RLSCMPGIANFLQVAE | |
| 774.3964 | 1546.7782 | 1546.7641 | 9.18 | 1 | 16 | 0.82 | 1Score > 41 indicates identityScore > 27 indicates homology |
YQPQVGFNRNAPR + Deamidated (NQ) | |
| 938.4541 | 1874.8936 | 1874.9110 | -9.24 | 1 | 16 | 0.75 | 1Score > 40 indicates identityScore > 27 indicates homology |
QIEVTTDGAGVSGTVVDVE | |
| 738.9022 | 1475.7898 | 1475.7620 | 18.9 | 0 | 16 | 0.69 | 1Score > 40 indicates identityScore > 27 indicates homology |
ANNAILQFLDISR + 2 Deamidated (NQ) | |
| 712.3610 | 1422.7074 | 1422.7354 | -19.7 | 1 | 16 | 0.58 | 1Score > 41 indicates identityScore > 26 indicates homology |
LQSQIQSKFQSK + 2 Deamidated (NQ) | |
| 569.3069 | 568.2996 | 568.2969 | 4.78 | 0 | 16 | 0.32 | 1Score > 38 indicates identityScore > 23 indicates homology |
DVHAK | |
| 413.5601 | 1237.6585 | 1237.6666 | -6.59 | 1 | 16 | 0.51 | 1Score > 39 indicates identityScore > 25 indicates homology |
ENLDGLIHISK | |
| 769.9156 | 1537.8166 | 1537.8212 | -2.99 | 0 | 16 | 0.64 | 1Score > 39 indicates identityScore > 26 indicates homology |
TTLPSQGPLQRPSR + Deamidated (NQ) | |
| 693.7088 | 2078.1046 | 2078.0983 | 3.03 | 0 | 16 | 0.61 | 1Score > 37 indicates identityScore > 26 indicates homology |
LNSAVFPGGQGGPLMHIIAAK + Deamidated (NQ) | |
| 417.9066 | 1250.6980 | 1250.6758 | 17.7 | 1 | 16 | 0.49 | 1Score > 37 indicates identityScore > 25 indicates homology |
SSLLPKLTTYE | |
| 438.5667 | 1312.6783 | 1312.6623 | 12.2 | 0 | 16 | 0.63 | 1Score > 40 indicates identityScore > 26 indicates homology |
VEPGLGADNSVVR + Deamidated (NQ) | |
| 380.2542 | 758.4938 | 758.4902 | 4.85 | 0 | 16 | 0.7 | 1Score > 30 indicates identityScore > 27 indicates homology |
VISSLIK | |
| 748.8718 | 1495.7290 | 1495.7129 | 10.8 | 0 | 16 | 0.36 | 1Score > 41 indicates identityScore > 24 indicates homology |
FMIAANGFEPDLR + Oxidation (M) | |
| 748.8997 | 1495.7848 | 1495.8021 | -11.5 | 1 | 16 | 0.9 | 1Score > 40 indicates identityScore > 28 indicates homology |
IPIELLQVNDIVE + 2 Deamidated (NQ) | |
| 430.7487 | 859.4828 | 859.4837 | -1.00 | 0 | 16 | 0.88 | 1Score > 41 indicates identityScore > 27 indicates homology |
MLDAAVIK | |
| 580.3617 | 1158.7088 | 1158.7046 | 3.67 | 1 | 16 | 0.086 | 1Score > 31 indicates identityScore > 17 indicates homology |
SLPLLMITKK + Oxidation (M) | |
| 897.9713 | 1793.9280 | 1793.9523 | -13.5 | 1 | 16 | 0.33 | 1Score > 39 indicates identityScore > 23 indicates homology |
QQDIPVENVLALGGIAR + 2 Deamidated (NQ) | |
| 799.4194 | 1596.8242 | 1596.7968 | 17.2 | 0 | 16 | 0.68 | 1Score > 40 indicates identityScore > 26 indicates homology |
QGQQQQQLQQQQK | |
| 410.5762 | 1228.7068 | 1228.7139 | -5.81 | 1 | 16 | 0.83 | 1Score > 38 indicates identityScore > 27 indicates homology |
QISSLGQQIKK | |
| 555.2968 | 1662.8686 | 1662.8537 | 8.97 | 1 | 16 | 0.27 | 1Score > 40 indicates identityScore > 22 indicates homology |
ISGASGLASNTTNTKNK | |
| 693.7086 | 2078.1040 | 2078.0983 | 2.75 | 0 | 15 | 0.14 | 1Score > 37 indicates identityScore > 19 indicates homology |
LNSAVFPGGQGGPLMHIIAAK + Deamidated (NQ) | |
| 528.2869 | 1054.5592 | 1054.5407 | 17.6 | 1 | 15 | 0.66 | 1Score > 39 indicates identityScore > 26 indicates homology |
NSIETQVHK | |
| 450.2890 | 898.5634 | 898.5600 | 3.87 | 0 | 15 | 0.7 | 1Score > 37 indicates identityScore > 26 indicates homology |
ANLLSILR | |
| 1006.5301 | 2011.0456 | 2011.0473 | -0.85 | 1 | 15 | 0.66 | 1Score > 39 indicates identityScore > 26 indicates homology |
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ) | |
| 352.4958 | 1054.4656 | 1054.4866 | -19.9 | 0 | 15 | 0.45 | 1Score > 39 indicates identityScore > 24 indicates homology |
GAFLSNGSMR + Oxidation (M) | |
| 636.8414 | 1271.6682 | 1271.6479 | 16.0 | 0 | 15 | 0.45 | 1Score > 41 indicates identityScore > 24 indicates homology |
GLLHCMVTSVR | |
| 458.8860 | 1373.6362 | 1373.6575 | -15.5 | 0 | 15 | 0.12 | 1Score > 40 indicates identityScore > 19 indicates homology |
TSIEPDGSLWNR | |
| 518.2980 | 1034.5814 | 1034.6012 | -19.1 | 1 | 15 | 0.37 | 1Score > 38 indicates identityScore > 24 indicates homology |
IEIYIVGTK | |
| 319.5147 | 955.5223 | 955.5312 | -9.30 | 1 | 15 | 0.64 | 1Score > 39 indicates identityScore > 26 indicates homology |
GRGNAQVVR | |
| 391.2047 | 1170.5923 | 1170.5782 | 12.0 | 1 | 15 | 0.64 | 1Score > 40 indicates identityScore > 26 indicates homology |
FHGVADQELR | |
| 610.3054 | 1218.5962 | 1218.5728 | 19.3 | 1 | 15 | 0.84 | 1Score > 42 indicates identityScore > 27 indicates homology |
NTAEVAQGAASAK + 2 Deamidated (NQ) | |
| 442.2681 | 1323.7825 | 1323.7809 | 1.20 | 1 | 15 | 0.43 | 1Score > 33 indicates identityScore > 24 indicates homology |
GVIAMLARQLPR | |
| 570.2929 | 1707.8569 | 1707.8389 | 10.5 | 0 | 15 | 0.92 | 1Score > 40 indicates identityScore > 27 indicates homology |
TSFQMLPISNEPISK + Deamidated (NQ); Oxidation (M) | |
| 904.4619 | 1806.9092 | 1806.9363 | -15.0 | 1 | 15 | 0.52 | 1Score > 40 indicates identityScore > 25 indicates homology |
QAGVPIVPGSQGIIENVE + Deamidated (NQ) | |
| 633.8837 | 1265.7528 | 1265.7343 | 14.6 | 0 | 15 | 0.65 | 1Score > 33 indicates identityScore > 26 indicates homology |
LQLQQLGLVPR + 2 Deamidated (NQ) | |
| 476.2492 | 950.4838 | 950.4709 | 13.6 | 1 | 15 | 0.85 | 1Score > 41 indicates identityScore > 27 indicates homology |
AGEIVYDGK | |
| 423.2246 | 844.4346 | 844.4290 | 6.67 | 1 | 15 | 0.77 | 1Score > 43 indicates identityScore > 27 indicates homology |
NVANIGEK + Deamidated (NQ) | |
| 304.4160 | 1213.6349 | 1213.6489 | -11.5 | 1 | 15 | 0.37 | 1Score > 40 indicates identityScore > 23 indicates homology |
MINPNLVLER + Oxidation (M) | |
| 323.8391 | 968.4955 | 968.5039 | -8.74 | 0 | 15 | 0.69 | 1Score > 39 indicates identityScore > 26 indicates homology |
GSTGAPPNLR | |
| 728.4357 | 1454.8568 | 1454.8569 | -0.059 | 1 | 15 | 0.62 | 1Score > 33 indicates identityScore > 26 indicates homology |
VSVTVLGVKGNQVR | |
| 379.2166 | 1512.8373 | 1512.8512 | -9.16 | 0 | 15 | 0.5 | 1Score > 38 indicates identityScore > 25 indicates homology |
TSGDLLAGLLLDGLR | |
| 671.3558 | 2011.0456 | 2011.0262 | 9.63 | 1 | 15 | 0.47 | 1Score > 39 indicates identityScore > 24 indicates homology |
QTLSPSVLIEHLNLFGNE + Deamidated (NQ) | |
| 1068.5627 | 2135.1108 | 2135.0858 | 11.7 | 1 | 15 | 0.15 | 1Score > 38 indicates identityScore > 19 indicates homology |
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ) | |
| 593.3312 | 1184.6478 | 1184.6448 | 2.59 | 1 | 15 | 0.84 | 1Score > 40 indicates identityScore > 27 indicates homology |
NCQALRAKPK | |
| 736.8672 | 1471.7198 | 1471.7381 | -12.4 | 0 | 15 | 0.33 | 1Score > 40 indicates identityScore > 23 indicates homology |
WALMIDPQGQALK + 2 Deamidated (NQ) | |
| 586.3884 | 585.3811 | 585.3737 | 12.6 | 0 | 15 | 0.23 | 1Score > 39 indicates identityScore > 21 indicates homology |
ILDIL | |
| 380.2561 | 758.4976 | 758.4902 | 9.86 | 0 | 15 | 0.8 | 1Score > 28 indicates identityScore > 27 indicates homology |
SVLSILK | |
| 308.6877 | 615.3608 | 615.3592 | 2.74 | 0 | 15 | 0.9 | 1Score > 45 indicates identityScore > 27 indicates homology |
IVDAAK | |
| 456.5702 | 1366.6888 | 1366.6915 | -1.98 | 0 | 15 | 0.74 | 1Score > 40 indicates identityScore > 26 indicates homology |
GTYVCIATNALGK | |
| 750.4482 | 1498.8818 | 1498.8541 | 18.5 | 1 | 15 | 0.18 | 1Score > 32 indicates identityScore > 20 indicates homology |
NKLLIIDGMNLVR + Deamidated (NQ) | |
| 1031.5144 | 1030.5071 | 1030.5196 | -12.1 | 0 | 15 | 0.71 | 1Score > 41 indicates identityScore > 26 indicates homology |
NGVWSIAAGR + Deamidated (NQ) | |
| 752.3866 | 1502.7586 | 1502.7576 | 0.68 | 1 | 15 | 0.48 | 1Score > 41 indicates identityScore > 24 indicates homology |
TSLTLADIQDAEAR | |
| 671.3558 | 2011.0456 | 2011.0262 | 9.63 | 1 | 15 | 0.46 | 1Score > 39 indicates identityScore > 24 indicates homology |
QTLSPSVLIEHLNLFGNE + Deamidated (NQ) | |
| 683.8416 | 1365.6686 | 1365.6646 | 2.99 | 1 | 15 | 0.27 | 1Score > 41 indicates identityScore > 22 indicates homology |
GRHTVPCMPPAK + Oxidation (M) | |
| 336.7014 | 671.3882 | 671.3966 | -12.4 | 1 | 15 | 0.69 | 1Score > 42 indicates identityScore > 26 indicates homology |
KGAATPK | |
| 854.4759 | 853.4686 | 853.4657 | 3.37 | 1 | 15 | 0.97 | 1Score > 38 indicates identityScore > 27 indicates homology |
EHLVASAK | |
| 704.3737 | 2110.0993 | 2110.0921 | 3.40 | 1 | 15 | 0.28 | 1Score > 38 indicates identityScore > 22 indicates homology |
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M) | |
| 398.1991 | 794.3836 | 794.3810 | 3.32 | 1 | 15 | 0.57 | 1Score > 41 indicates identityScore > 25 indicates homology |
AVFENSK + Deamidated (NQ) | |
| 825.9319 | 1649.8492 | 1649.8777 | -17.2 | 0 | 15 | 0.71 | 1Score > 40 indicates identityScore > 26 indicates homology |
AIQNSFFLLINDVR + Deamidated (NQ) | |
| 532.2680 | 1593.7822 | 1593.7787 | 2.17 | 0 | 15 | 0.76 | 1Score > 41 indicates identityScore > 26 indicates homology |
DAYAVTPFNTPTAAR | |
| 862.4822 | 2584.4248 | 2584.3999 | 9.61 | 1 | 15 | 1 | 1Score > 32 indicates identityScore > 27 indicates homology |
SKGSATALYSQLLDTTLQILYLGK + Deamidated (NQ) | |
| 440.7268 | 879.4390 | 879.4338 | 5.98 | 0 | 15 | 0.78 | 1Score > 42 indicates identityScore > 26 indicates homology |
DAGVDIYK | |
| 684.3527 | 1366.6908 | 1366.6803 | 7.75 | 1 | 15 | 0.8 | 1Score > 40 indicates identityScore > 26 indicates homology |
TSGFEMVQGLLGK + Deamidated (NQ) | |
| 577.0298 | 2304.0901 | 2304.1096 | -8.49 | 1 | 15 | 0.79 | 1Score > 39 indicates identityScore > 26 indicates homology |
VVTDKAPTDDQWDAMLLGWK + Oxidation (M) | |
| 990.5140 | 989.5067 | 989.5215 | -15.0 | 1 | 15 | 0.4 | 1Score > 42 indicates identityScore > 23 indicates homology |
KVNLGMSLE | |
| 665.3514 | 1328.6882 | 1328.6936 | -4.02 | 1 | 15 | 0.3 | 1Score > 41 indicates identityScore > 22 indicates homology |
QVVVNLLGSRGGE + 2 Deamidated (NQ) | |
| 755.9507 | 1509.8868 | 1509.8654 | 14.2 | 0 | 15 | 0.17 | 1Score > 32 indicates identityScore > 20 indicates homology |
LIVLTDTPGILLNE | |
| 475.7536 | 949.4926 | 949.4869 | 6.09 | 1 | 15 | 0.76 | 1Score > 42 indicates identityScore > 26 indicates homology |
LDAYEALR | |
| 586.8334 | 1171.6522 | 1171.6448 | 6.34 | 1 | 15 | 0.47 | 1Score > 40 indicates identityScore > 24 indicates homology |
NLSAAVLDELK | |
| 1017.0264 | 2032.0382 | 2032.0364 | 0.91 | 0 | 15 | 0.16 | 1Score > 39 indicates identityScore > 19 indicates homology |
ILIHINNTNPILDLDSPE + 2 Deamidated (NQ) | |
| 510.6254 | 1528.8544 | 1528.8402 | 9.27 | 0 | 15 | 0.29 | 1Score > 37 indicates identityScore > 22 indicates homology |
AFNIDLGFPLIGPR | |
| 766.9457 | 1531.8768 | 1531.8511 | 16.8 | 1 | 15 | 0.63 | 1Score > 34 indicates identityScore > 25 indicates homology |
SRVGIVSWGAYIPK | |
| 471.7317 | 1882.8977 | 1882.8818 | 8.44 | 0 | 15 | 0.52 | 1Score > 40 indicates identityScore > 24 indicates homology |
LCSLNINMHHDPFLR + Deamidated (NQ); Oxidation (M) | |
| 538.2720 | 1611.7942 | 1611.7933 | 0.57 | 0 | 15 | 0.35 | 1Score > 40 indicates identityScore > 23 indicates homology |
ANFLAISGYIWNNK + 2 Deamidated (NQ) | |
| 501.3167 | 500.3094 | 500.3071 | 4.68 | 0 | 15 | 0.23 | 1Score > 43 indicates identityScore > 21 indicates homology |
AVVGR | |
| 403.7245 | 805.4344 | 805.4334 | 1.31 | 0 | 15 | 0.8 | 1Score > 41 indicates identityScore > 26 indicates homology |
LFNDLGK |
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