MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1488 560.8093 1119.6040 1119.5924 10.4 1 18 0.83 +1Score > 40 indicates identity
Score > 30 indicates homology		 VAQLYLEQR + Deamidated (NQ)
979 302.8481 905.5225 905.5334 -12.1 1 18 0.89 +1Score > 38 indicates identity
Score > 30 indicates homology		 RLFQTLK + Deamidated (NQ)
1486 560.8057 1119.5968 1119.5924 3.97 0 18 0.49 +1Score > 41 indicates identity
Score > 28 indicates homology		 GAVVAYANDLK
1815 633.8824 1265.7502 1265.7343 12.6 1 18 0.28 +1Score > 33 indicates identity
Score > 25 indicates homology		 LQLQELGPVIR + Deamidated (NQ)
765 409.7181 817.4216 817.4293 -9.41 1 18 0.69 +1Score > 44 indicates identity
Score > 29 indicates homology		 KNSNNIK + Deamidated (NQ)
1826 637.8118 1273.6090 1273.5972 9.27 1 18 0.18 +1Score > 41 indicates identity
Score > 23 indicates homology		 QPIMQGDTEQK
2836 586.9987 1757.9743 1757.9828 -4.88 0 18 0.62 +1Score > 36 indicates identity
Score > 29 indicates homology		 VSVIPTLLHVAGYSFR
829 423.2502 844.4858 844.4840 2.16 1 18 0.82 +1Score > 40 indicates identity
Score > 30 indicates homology		 LIAEMLR
2643 826.4551 1650.8956 1650.8828 7.76 0 18 0.15 +1Score > 37 indicates identity
Score > 23 indicates homology		 VSLNTAAVLHPSLVTE + Deamidated (NQ)
2465 790.4496 1578.8846 1578.8729 7.42 1 18 0.96 +1Score > 36 indicates identity
Score > 31 indicates homology		 AAVQEGIVPGGGIALAR + Deamidated (NQ)
1806 421.5735 1261.6987 1261.6918 5.46 1 18 0.59 +1Score > 39 indicates identity
Score > 29 indicates homology		 QIDELFLSAVK
365 316.1808 630.3470 630.3449 3.37 0 18 0.3 +1Score > 43 indicates identity
Score > 26 indicates homology		 AGGAVTR
2414 778.4425 1554.8704 1554.8869 -10.6 1 18 0.88 +1Score > 37 indicates identity
Score > 30 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
404 329.7109 657.4072 657.4061 1.73 1 18 1 +1Score > 41 indicates identity
Score > 31 indicates homology		 AVEVIK
2039 686.3431 1370.6716 1370.6929 -15.5 1 18 0.99 +1Score > 40 indicates identity
Score > 31 indicates homology		 LNLNLQDEAVIE + Deamidated (NQ)
2360 510.6248 1528.8526 1528.8249 18.1 0 18 0.62 +1Score > 37 indicates identity
Score > 29 indicates homology		 FNHQLTDLTSIIK
1578 579.3022 1156.5898 1156.6088 -16.4 1 18 0.95 +1Score > 40 indicates identity
Score > 31 indicates homology		 VQQVVDAAAKE
998 305.8324 914.4754 914.4610 15.7 0 18 0.56 +1Score > 41 indicates identity
Score > 28 indicates homology		 DIHNFIR + Deamidated (NQ)
2391 772.4335 1542.8524 1542.8439 5.53 0 18 0.51 +1Score > 38 indicates identity
Score > 28 indicates homology		 NMVVSNILLNNIAK + Deamidated (NQ)
3710 1152.0688 2302.1230 2302.1176 2.36 1 18 0.25 +1Score > 39 indicates identity
Score > 25 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
1431 544.3121 1086.6096 1086.6073 2.13 0 18 0.33 +1Score > 40 indicates identity
Score > 26 indicates homology		 VPIQIQNFK + Deamidated (NQ)
1409 539.2680 1076.5214 1076.5349 -12.5 1 18 0.18 +1Score > 42 indicates identity
Score > 23 indicates homology		 KTQDNTAAVK + 2 Deamidated (NQ)
943 447.7391 893.4636 893.4607 3.34 1 18 0.91 +1Score > 41 indicates identity
Score > 30 indicates homology		 LSGELYGR
1214 496.2852 990.5558 990.5498 6.09 0 18 0.81 +1Score > 40 indicates identity
Score > 30 indicates homology		 AIVIGFNTR + Deamidated (NQ)
1672 600.3416 1198.6686 1198.6921 -19.6 0 18 0.49 +1Score > 40 indicates identity
Score > 27 indicates homology		 VDIIALDATLR
2695 837.4702 1672.9258 1672.9069 11.3 1 18 0.11 +1Score > 36 indicates identity
Score > 21 indicates homology		 KLVILGGAMQGNVLDK + 2 Deamidated (NQ); Oxidation (M)
1721 408.1901 1221.5485 1221.5634 -12.3 0 18 0.87 +1Score > 40 indicates identity
Score > 30 indicates homology		 MADIMHVFAR + 2 Oxidation (M)
3685 765.3943 2293.1611 2293.1240 16.2 1 18 0.12 +1Score > 38 indicates identity
Score > 21 indicates homology		 FWLGNDHIHALTAQGTNELR + Deamidated (NQ)
1474 557.8107 1113.6068 1113.6030 3.50 0 18 0.61 +1Score > 41 indicates identity
Score > 28 indicates homology		 AIVAQVLQNR + 3 Deamidated (NQ)
2742 851.9225 1701.8304 1701.8421 -6.85 1 18 0.82 +1Score > 40 indicates identity
Score > 30 indicates homology		 DLTQQAKDIQNVTVE + Deamidated (NQ)
870 429.7702 857.5258 857.5222 4.23 0 18 0.64 +1Score > 37 indicates identity
Score > 28 indicates homology		 TQVIAVVK + Deamidated (NQ)
1237 333.8583 998.5531 998.5437 9.41 1 18 0.97 +1Score > 39 indicates identity
Score > 30 indicates homology		 KIYDFVSK
1279 507.7935 1013.5724 1013.5869 -14.3 1 18 0.5 +1Score > 40 indicates identity
Score > 27 indicates homology		 VVSEALGALR
519 352.2223 702.4300 702.4276 3.51 0 18 0.83 +1Score > 38 indicates identity
Score > 30 indicates homology		 ITTAGIK
1657 597.3425 1192.6704 1192.6676 2.36 1 18 0.77 +1Score > 37 indicates identity
Score > 29 indicates homology		 GALEVRPPQAR
2112 468.9143 1403.7211 1403.6946 18.9 0 18 0.31 +1Score > 41 indicates identity
Score > 25 indicates homology		 NFHQHQITHLK + 2 Deamidated (NQ)
238 516.3050 515.2977 515.2955 4.34 0 18 0.55 +1Score > 47 indicates identity
Score > 28 indicates homology		 ALAQL + Deamidated (NQ)
3063 944.5141 1887.0136 1887.0102 1.85 1 18 0.29 +1Score > 38 indicates identity
Score > 25 indicates homology		 FGRLVQLQTLILQDNE + Deamidated (NQ)
2090 695.8896 1389.7646 1389.7616 2.21 1 18 0.47 +1Score > 38 indicates identity
Score > 27 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
2479 528.2680 1581.7822 1581.8038 -13.7 1 18 0.22 +1Score > 41 indicates identity
Score > 24 indicates homology		 AGPLPPLNQEAAFQK + 2 Deamidated (NQ)
657 383.6918 765.3690 765.3657 4.37 1 18 0.96 +1Score > 42 indicates identity
Score > 30 indicates homology		 VESFQR + Deamidated (NQ)
2068 462.9209 1385.7409 1385.7402 0.50 1 18 0.39 +1Score > 40 indicates identity
Score > 26 indicates homology		 SLATLASEQPIQK + Deamidated (NQ)
2809 578.5974 1732.7704 1732.7951 -14.3 1 18 0.1 +1Score > 38 indicates identity
Score > 20 indicates homology		 HNQSPRNTYQSAMAK + Deamidated (NQ)
2705 562.2964 1683.8674 1683.8977 -18.0 1 18 0.091 +1Score > 40 indicates identity
Score > 20 indicates homology		 ALASNRTLSMLHLQK + 2 Deamidated (NQ)
517 352.2038 702.3930 702.3912 2.65 0 18 0.98 +1Score > 44 indicates identity
Score > 30 indicates homology		 AGDILSK
1964 669.8274 1337.6402 1337.6649 -18.5 1 18 0.38 +1Score > 41 indicates identity
Score > 26 indicates homology		 MEFQTTLPSIR + Oxidation (M)
2106 701.3998 1400.7850 1400.7875 -1.72 1 18 0.47 +1Score > 38 indicates identity
Score > 27 indicates homology		 KVTSNIKPQSLGK + 2 Deamidated (NQ)
838 425.7161 849.4176 849.4344 -19.8 0 18 0.15 +1Score > 43 indicates identity
Score > 22 indicates homology		 SLDAFAAR
2027 683.8632 1365.7118 1365.7292 -12.7 0 18 0.53 +1Score > 40 indicates identity
Score > 27 indicates homology		 IAAQDYIIGVFR + Deamidated (NQ)
1761 411.8965 1232.6677 1232.6652 1.99 0 18 0.58 +1Score > 40 indicates identity
Score > 28 indicates homology		 TLNLLFALAQE + Deamidated (NQ)
2483 793.4344 1584.8542 1584.8512 1.94 0 18 0.55 +1Score > 39 indicates identity
Score > 28 indicates homology		 TVILWDVAQGQVVR + 2 Deamidated (NQ)
3222 1006.4950 2010.9754 2010.9660 4.69 0 18 0.84 +1Score > 40 indicates identity
Score > 29 indicates homology		 QLTHQQIYPHFADGSNR
754 407.2290 812.4434 812.4504 -8.59 1 18 0.36 +1Score > 39 indicates identity
Score > 26 indicates homology		 KAPASSPR
2822 871.9962 1741.9778 1741.9726 3.00 1 18 0.51 +1Score > 35 indicates identity
Score > 27 indicates homology		 QIQLLLQYLKNDPR + Deamidated (NQ)
1364 527.8350 1053.6554 1053.6434 11.5 0 18 0.32 +1Score > 33 indicates identity
Score > 25 indicates homology		 VAEPLITLAK
1813 422.9208 1265.7406 1265.7417 -0.90 0 18 0.86 +1Score > 34 indicates identity
Score > 30 indicates homology		 DLPLIGMPIAVK
3202 1000.0320 1998.0494 1998.0171 16.2 0 18 0.26 +1Score > 38 indicates identity
Score > 24 indicates homology		 LVIDAGNTNIVFAVHDGSR + Deamidated (NQ)
3132 488.7500 1950.9709 1950.9660 2.51 1 18 0.15 +1Score > 40 indicates identity
Score > 22 indicates homology		 SNRHIYAQVIDDVHQR + Deamidated (NQ)
730 400.7132 799.4118 799.4076 5.36 1 18 0.19 +1Score > 39 indicates identity
Score > 23 indicates homology		 KAGADPIE
1432 544.7721 1087.5296 1087.5444 -13.6 0 18 0.65 +1Score > 42 indicates identity
Score > 28 indicates homology		 GLCLTQPGSR
2722 565.2868 1692.8386 1692.8393 -0.42 0 18 0.19 +1Score > 41 indicates identity
Score > 23 indicates homology		 ALTVPDLTQQMFDAK + Oxidation (M)
811 419.7455 837.4764 837.4821 -6.70 1 18 0.46 +1Score > 36 indicates identity
Score > 27 indicates homology		 AKIHNGAK
3380 1056.0490 2110.0834 2110.0946 -5.29 1 18 0.13 +1Score > 38 indicates identity
Score > 21 indicates homology		 AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
1865 430.5897 1288.7473 1288.7391 6.36 0 18 0.63 +1Score > 35 indicates identity
Score > 28 indicates homology		 TFSIIKPDAVAK
1012 460.2504 918.4862 918.4923 -6.58 0 18 0.82 +1Score > 42 indicates identity
Score > 29 indicates homology		 IGNIAQFR + Deamidated (NQ)
1660 598.3884 1194.7622 1194.7448 14.6 0 18 0.12 +1Score > 27 indicates identity
Score > 21 indicates homology		 IVVALGGNALLR
502 351.6936 701.3726 701.3821 -13.4 0 18 0.92 +1Score > 45 indicates identity
Score > 30 indicates homology		 VQSGVGR
2758 570.3244 1707.9514 1707.9407 6.26 0 18 0.59 +1Score > 36 indicates identity
Score > 28 indicates homology		 LQNGLTPLLIVTPNGR + 3 Deamidated (NQ)
1305 343.1947 1026.5623 1026.5498 12.1 1 18 0.19 +1Score > 39 indicates identity
Score > 23 indicates homology		 RVYDTFVK
1393 357.8469 1070.5189 1070.5356 -15.6 1 18 0.38 +1Score > 40 indicates identity
Score > 26 indicates homology		 KNIDPQNNK + Deamidated (NQ)
1559 575.7826 1149.5506 1149.5448 5.10 1 18 0.69 +1Score > 42 indicates identity
Score > 28 indicates homology		 LMNQSQGSRK + 2 Deamidated (NQ)
333 616.3440 615.3367 615.3452 -13.8 1 17 0.89 +1Score > 44 indicates identity
Score > 29 indicates homology		 KAGNAR
663 385.7312 769.4478 769.4446 4.19 0 17 0.83 +1Score > 37 indicates identity
Score > 29 indicates homology		 LGQSLPR
1440 365.5257 1093.5553 1093.5404 13.6 0 17 0.52 +1Score > 41 indicates identity
Score > 27 indicates homology		 NSYLIDNVR + Deamidated (NQ)
1604 391.2050 1170.5932 1170.5768 14.0 1 17 0.16 +1Score > 40 indicates identity
Score > 22 indicates homology		 IAQALVENPSE + Deamidated (NQ)
2359 510.6191 1528.8355 1528.8283 4.69 1 17 0.9 +1Score > 39 indicates identity
Score > 30 indicates homology		 NVIVLQTVMQEVR + Deamidated (NQ)
1148 322.1758 963.5056 963.4881 18.1 0 17 0.82 +1Score > 41 indicates identity
Score > 29 indicates homology		 QMGLIMQK + Oxidation (M)
1691 604.7814 1207.5482 1207.5576 -7.78 1 17 0.98 +1Score > 41 indicates identity
Score > 30 indicates homology		 MANAANMIKVE + Deamidated (NQ); Oxidation (M)
1271 337.8671 1010.5795 1010.5621 17.2 1 17 0.37 +1Score > 36 indicates identity
Score > 26 indicates homology		 SLRAAPGSPR
1500 375.8663 1124.5771 1124.5866 -8.45 0 17 0.59 +1Score > 40 indicates identity
Score > 28 indicates homology		 IAVNYQLFR + 2 Deamidated (NQ)
915 440.2530 878.4914 878.4749 18.8 1 17 0.4 +1Score > 38 indicates identity
Score > 26 indicates homology		 YLINEVK + Deamidated (NQ)
2385 772.4255 1542.8364 1542.8617 -16.4 0 17 0.69 +1Score > 39 indicates identity
Score > 28 indicates homology		 SGATLNQLLVQSAIK + Deamidated (NQ)
988 912.4719 911.4646 911.4752 -11.7 1 17 0.14 +1Score > 40 indicates identity
Score > 21 indicates homology		 KFDINFK + Deamidated (NQ)
2235 490.9319 1469.7739 1469.7772 -2.28 1 17 0.33 +1Score > 40 indicates identity
Score > 25 indicates homology		 MNAQPRSALALAAR + Deamidated (NQ)
1110 316.8563 947.5471 947.5440 3.25 0 17 0.73 +1Score > 38 indicates identity
Score > 28 indicates homology		 QYLLLNGK
2564 539.3037 1614.8893 1614.8585 19.0 1 17 0.52 +1Score > 38 indicates identity
Score > 27 indicates homology		 IMNMNQRLLLPVR + 2 Deamidated (NQ); Oxidation (M)
744 401.7220 801.4294 801.4232 7.77 1 17 0.93 +1Score > 42 indicates identity
Score > 29 indicates homology		 LQSETPK
702 392.7701 783.5256 783.5331 -9.45 1 17 0.92 +1Score > 32 indicates identity
Score > 29 indicates homology		 LGIVVKR
1380 354.8587 1061.5543 1061.5505 3.52 0 17 0.2 +1Score > 41 indicates identity
Score > 23 indicates homology		 AANAPVYVTR + Deamidated (NQ)
2191 722.3755 1442.7364 1442.7365 -0.033 1 17 0.11 +1Score > 41 indicates identity
Score > 20 indicates homology		 AIADLVDAETAAQR
2648 828.9894 1655.9642 1655.9458 11.2 1 17 0.37 +1Score > 32 indicates identity
Score > 25 indicates homology		 ILSTRSQIVGIDLIE
1320 517.7507 1033.4868 1033.4803 6.29 0 17 0.76 +1Score > 41 indicates identity
Score > 29 indicates homology		 QYMWLHR + Deamidated (NQ)
1876 648.3782 1294.7418 1294.7609 -14.7 1 17 0.46 +1Score > 35 indicates identity
Score > 26 indicates homology		 VVGLSTQLPPRK + Deamidated (NQ)
1294 510.2831 1018.5516 1018.5335 17.8 0 17 0.55 +1Score > 41 indicates identity
Score > 27 indicates homology		 AGFNLLLAAE + Deamidated (NQ)
2340 758.3723 1514.7300 1514.7293 0.48 1 17 0.34 +1Score > 41 indicates identity
Score > 25 indicates homology		 VFSVFALFENDVE
1669 600.3353 1198.6560 1198.6418 11.9 1 17 0.59 +1Score > 40 indicates identity
Score > 27 indicates homology		 DVLALARDGNR
531 354.6895 707.3644 707.3602 5.96 0 17 0.74 +1Score > 41 indicates identity
Score > 28 indicates homology		 FIADSR
347 312.1740 622.3334 622.3439 -16.7 0 17 0.096 +1Score > 41 indicates identity
Score > 19 indicates homology		 ITFSR
258 547.3243 546.3170 546.3126 8.17 0 17 0.5 +1Score > 38 indicates identity
Score > 27 indicates homology		 AVTTR
902 437.2639 872.5132 872.4967 19.0 1 17 0.15 +1Score > 40 indicates identity
Score > 21 indicates homology		 QIAAEITK
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