MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1021 461.7372 921.4598 921.4630 -3.38 0 20 0.69 +1Score > 42 indicates identity
Score > 31 indicates homology		 IWQIMSK + Deamidated (NQ); Oxidation (M)
1256 504.7441 1007.4736 1007.4924 -18.6 1 20 0.7 +1Score > 42 indicates identity
Score > 31 indicates homology		 GASLADFAEK
1722 611.8097 1221.6048 1221.6023 2.08 1 20 0.51 +1Score > 42 indicates identity
Score > 30 indicates homology		 QKGLSSAMLDR + Deamidated (NQ); Oxidation (M)
459 339.7015 677.3884 677.3860 3.54 0 20 0.57 +1Score > 40 indicates identity
Score > 30 indicates homology		 IYGGLR
1696 405.2042 1212.5908 1212.5696 17.5 0 20 0.19 +1Score > 40 indicates identity
Score > 25 indicates homology		 MLYQQIASNK + 2 Deamidated (NQ); Oxidation (M)
521 352.2226 702.4306 702.4276 4.36 1 20 0.99 +1Score > 38 indicates identity
Score > 33 indicates homology		 IDKVTK
1957 667.8605 1333.7064 1333.6990 5.59 1 20 0.17 +1Score > 40 indicates identity
Score > 25 indicates homology		 SFLVNLDRNQK + Deamidated (NQ)
2089 695.8888 1389.7630 1389.7616 1.06 1 20 0.85 +1Score > 39 indicates identity
Score > 32 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
1671 600.3394 1198.6642 1198.6418 18.7 1 20 0.95 +1Score > 40 indicates identity
Score > 32 indicates homology		 DVLALARDGNR
2083 695.8288 1389.6430 1389.6307 8.90 0 20 0.52 +1Score > 40 indicates identity
Score > 30 indicates homology		 QGSDIDAACGQLR
1979 672.8528 1343.6910 1343.6905 0.38 1 20 0.92 +1Score > 41 indicates identity
Score > 32 indicates homology		 ILSSNGRNVNNR + Deamidated (NQ)
962 300.8199 899.4379 899.4501 -13.6 0 20 0.45 +1Score > 39 indicates identity
Score > 29 indicates homology		 DAWGPGVAK
1053 465.2781 928.5416 928.5454 -4.02 1 20 0.88 +1Score > 40 indicates identity
Score > 32 indicates homology		 IINSARQK
1288 339.4994 1015.4764 1015.4822 -5.72 1 20 0.14 +1Score > 40 indicates identity
Score > 24 indicates homology		 QPLNTGEQK + 2 Deamidated (NQ)
1779 414.2369 1239.6889 1239.6823 5.30 1 20 0.56 +1Score > 38 indicates identity
Score > 30 indicates homology		 RGADILLVGTPE
1859 644.3568 1286.6990 1286.7082 -7.08 0 20 0.24 +1Score > 40 indicates identity
Score > 26 indicates homology		 TVSLQALQQLGK + 2 Deamidated (NQ)
487 347.6811 693.3476 693.3446 4.43 1 20 0.64 +1Score > 41 indicates identity
Score > 31 indicates homology		 EGAYVR
946 447.7396 893.4646 893.4607 4.46 1 20 0.94 +1Score > 41 indicates identity
Score > 32 indicates homology		 LSGELYGR
1408 538.8055 1075.5964 1075.6026 -5.71 1 20 0.84 +1Score > 40 indicates identity
Score > 32 indicates homology		 IGGFEIATLR
3610 745.4406 2233.3000 2233.2583 18.7 1 20 0.23 +1Score > 27 indicates identity
Score > 26 indicates homology		 SLTRFGFTLIIGGAIGNLIDR
2964 610.3148 1827.9226 1827.9366 -7.70 0 20 0.28 +1Score > 40 indicates identity
Score > 27 indicates homology		 IPTLSQVQALSADWAAR + 2 Deamidated (NQ)
2975 613.6751 1838.0035 1837.9938 5.24 0 20 0.24 +1Score > 36 indicates identity
Score > 26 indicates homology		 GSVTNVLTSIVGNVFGFK
427 336.1898 670.3650 670.3762 -16.7 0 20 0.69 +1Score > 37 indicates identity
Score > 31 indicates homology		 AGPTVAR
3498 724.4381 2170.2925 2170.2911 0.61 0 20 0.18 +1Score > 25 indicates identity LIIFAMNGVLVAIAGLILSAR + Oxidation (M)
2660 833.4930 1664.9714 1664.9937 -13.4 1 20 0.51 +1Score > 32 indicates identity
Score > 29 indicates homology		 GILGDRIVAGDIVVIR
614 375.7124 749.4102 749.4072 4.09 0 20 0.5 +1Score > 42 indicates identity
Score > 29 indicates homology		 IFNTVR + Deamidated (NQ)
248 531.2910 530.2837 530.2813 4.67 1 20 0.9 +1Score > 46 indicates identity
Score > 32 indicates homology		 AEGVR
775 412.7366 823.4586 823.4664 -9.43 0 20 0.86 +1Score > 38 indicates identity
Score > 32 indicates homology		 HLASLGAR
882 431.2392 860.4638 860.4603 4.09 0 20 0.45 +1Score > 42 indicates identity
Score > 29 indicates homology		 TATIVNNK + Deamidated (NQ)
2547 807.4147 1612.8148 1612.8308 -9.90 1 20 0.71 +1Score > 40 indicates identity
Score > 31 indicates homology		 VTGALESGANAVLVSPE
835 424.7004 847.3862 847.3937 -8.75 1 20 0.38 +1Score > 41 indicates identity
Score > 28 indicates homology		 DDTWRR
2138 708.3634 1414.7122 1414.7245 -8.64 0 20 0.77 +1Score > 41 indicates identity
Score > 31 indicates homology		 LIWGVYPANEPR + Deamidated (NQ)
352 314.6754 627.3362 627.3340 3.56 0 20 0.57 +1Score > 37 indicates identity
Score > 30 indicates homology		 IGADPR
2028 683.9133 1365.8120 1365.7980 10.3 1 20 0.47 +1Score > 32 indicates identity
Score > 29 indicates homology		 QVLQKAPLALQR + 2 Deamidated (NQ)
293 586.3890 585.3817 585.3737 13.6 0 20 0.63 +1Score > 39 indicates identity
Score > 30 indicates homology		 ILDIL
958 450.2707 898.5268 898.5348 -8.89 1 20 0.37 +1Score > 39 indicates identity
Score > 28 indicates homology		 RVDAAVLR
2309 502.6065 1504.7977 1504.7773 13.5 1 20 0.74 +1Score > 39 indicates identity
Score > 31 indicates homology		 LYDLKAQGLDLQE
1289 508.7644 1015.5142 1015.5159 -1.63 1 20 0.42 +1Score > 42 indicates identity
Score > 28 indicates homology		 NAGDVAGRTR
468 343.2097 684.4048 684.3919 19.0 0 19 0.9 +1Score > 37 indicates identity
Score > 32 indicates homology		 GLGVSPR
2419 778.4558 1554.8970 1554.8729 15.5 1 19 0.67 +1Score > 35 indicates identity
Score > 30 indicates homology		 QQLKSAGSVLISAPR + Deamidated (NQ)
901 437.2164 872.4182 872.4352 -19.4 1 19 0.88 +1Score > 41 indicates identity
Score > 31 indicates homology		 QQAEIQR + Deamidated (NQ)
1226 332.8551 995.5435 995.5512 -7.79 0 19 0.94 +1Score > 37 indicates identity
Score > 32 indicates homology		 LSNVHTAVR
2466 790.4506 1578.8866 1578.8729 8.69 1 19 0.31 +1Score > 36 indicates identity
Score > 27 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
986 455.7575 909.5004 909.4960 4.89 1 19 0.28 +1Score > 38 indicates identity
Score > 26 indicates homology		 FLGGFLEK
1194 491.7411 981.4676 981.4556 12.3 1 19 0.53 +1Score > 40 indicates identity
Score > 29 indicates homology		 WEQYVTR + Deamidated (NQ)
627 380.2170 758.4194 758.4286 -12.1 0 19 0.98 +1Score > 44 indicates identity
Score > 32 indicates homology		 AVQNTVK
3445 1071.5734 2141.1322 2141.1255 3.14 1 19 0.13 +1Score > 37 indicates identity
Score > 23 indicates homology		 TLNLLLSYLNYSVDNIRK + 3 Deamidated (NQ)
1182 325.1954 972.5644 972.5604 4.12 1 19 0.56 +1Score > 39 indicates identity
Score > 29 indicates homology		 KLQQLSQK + Deamidated (NQ)
729 400.2390 798.4634 798.4487 18.5 1 19 0.38 +1Score > 38 indicates identity
Score > 28 indicates homology		 AEPIIKE
3612 746.4067 2236.1983 2236.2150 -7.49 1 19 0.19 +1Score > 36 indicates identity
Score > 25 indicates homology		 MFLQARAALVTGGALYIVGNR + Oxidation (M)
1848 427.5942 1279.7608 1279.7387 17.2 0 19 0.071 +1Score > 34 indicates identity
Score > 20 indicates homology		 AVSILNDLPPLK + Deamidated (NQ)
3206 1001.0350 2000.0554 2000.0289 13.3 1 19 0.28 +1Score > 38 indicates identity
Score > 26 indicates homology		 VILTPTHLAIVMEYASGGE
579 365.2257 728.4368 728.4293 10.4 1 19 0.97 +1Score > 41 indicates identity
Score > 32 indicates homology		 ALAGKNR
3012 930.9722 1859.9298 1859.9014 15.3 1 19 0.27 +1Score > 40 indicates identity
Score > 26 indicates homology		 APGQDVTLFRSPDSVDR + Deamidated (NQ)
2766 571.6678 1711.9816 1711.9608 12.2 1 19 0.22 +1Score > 34 indicates identity
Score > 25 indicates homology		 QQPITTTLELVDIIK + Deamidated (NQ)
1651 594.8342 1187.6538 1187.6510 2.42 1 19 0.65 +1Score > 40 indicates identity
Score > 30 indicates homology		 LTKSQLQQNK + Deamidated (NQ)
612 375.2025 748.3904 748.3901 0.42 1 19 0.99 +1Score > 42 indicates identity
Score > 32 indicates homology		 ISRMDK
438 336.7115 671.4084 671.4078 0.91 1 19 0.96 +1Score > 44 indicates identity
Score > 31 indicates homology		 AGAKGIR
680 388.7097 775.4048 775.4076 -3.50 0 19 0.66 +1Score > 42 indicates identity
Score > 30 indicates homology		 QSSNVIK + Deamidated (NQ)
3224 1006.4964 2010.9782 2010.9660 6.08 0 19 0.28 +1Score > 40 indicates identity
Score > 26 indicates homology		 QLTHQQIYPHFADGSNR
2961 609.2999 1824.8779 1824.8968 -10.4 0 19 0.53 +1Score > 40 indicates identity
Score > 29 indicates homology		 DVVEPYLIQQGFLMR + 2 Deamidated (NQ); Oxidation (M)
461 340.6808 679.3470 679.3442 4.20 0 19 0.39 +1Score > 40 indicates identity
Score > 27 indicates homology		 FPNFR
1243 501.7831 1001.5516 1001.5618 -10.1 1 19 0.37 +1Score > 41 indicates identity
Score > 27 indicates homology		 TQRDLTIR
1218 497.7718 993.5290 993.5356 -6.55 1 19 0.1 +1Score > 40 indicates identity
Score > 22 indicates homology		 DPKRPEPR
286 581.2701 580.2628 580.2605 3.98 0 19 0.63 +1Score > 40 indicates identity
Score > 29 indicates homology		 YADGR
2082 695.8282 1389.6418 1389.6372 3.35 1 19 0.4 +1Score > 40 indicates identity
Score > 28 indicates homology		 ADSLTAGGLDARDE
878 430.7486 859.4826 859.4837 -1.23 0 19 0.87 +1Score > 41 indicates identity
Score > 31 indicates homology		 LDMAVLAK
1363 527.8334 1053.6522 1053.6434 8.42 0 19 0.12 +1Score > 33 indicates identity
Score > 22 indicates homology		 VAEPLITLAK
1407 538.7672 1075.5198 1075.5033 15.4 1 19 0.67 +1Score > 42 indicates identity
Score > 30 indicates homology		 VTDAATAEVAE
601 372.1894 742.3642 742.3610 4.43 1 19 0.13 +1Score > 38 indicates identity
Score > 22 indicates homology		 GPEDGLR
1434 545.2842 1088.5538 1088.5502 3.35 1 19 0.88 +1Score > 42 indicates identity
Score > 31 indicates homology		 QQAQPFLEK + Deamidated (NQ)
1845 427.5677 1279.6813 1279.6594 17.1 1 19 0.47 +1Score > 39 indicates identity
Score > 28 indicates homology		 MPPIEPAERPK + Oxidation (M)
294 586.3893 585.3820 585.3737 14.1 0 19 0.62 +1Score > 39 indicates identity
Score > 29 indicates homology		 ILDIL
940 446.7445 891.4744 891.4814 -7.80 1 19 0.79 +1Score > 41 indicates identity
Score > 30 indicates homology		 VAEFLSAR
1723 611.8113 1221.6080 1221.6023 4.70 1 19 0.53 +1Score > 41 indicates identity
Score > 29 indicates homology		 QKGLSSAMLDR + Deamidated (NQ); Oxidation (M)
1976 448.6021 1342.7845 1342.7820 1.84 1 19 0.49 +1Score > 35 indicates identity
Score > 28 indicates homology		 TSLLRAVQQVVK + 2 Deamidated (NQ)
813 421.2624 840.5102 840.5181 -9.36 1 19 0.43 +1Score > 35 indicates identity
Score > 28 indicates homology		 KLQSLPR
947 894.4722 893.4649 893.4607 4.77 1 19 0.75 +1Score > 41 indicates identity
Score > 30 indicates homology		 GISEGLYR
1422 542.7879 1083.5612 1083.5560 4.82 0 19 0.35 +1Score > 40 indicates identity
Score > 27 indicates homology		 GSAPGQPVTAAK + Deamidated (NQ)
1670 600.3375 1198.6604 1198.6418 15.5 1 19 0.77 +1Score > 40 indicates identity
Score > 30 indicates homology		 DVLALARDGNR
1897 437.2220 1308.6442 1308.6561 -9.14 1 19 0.24 +1Score > 41 indicates identity
Score > 25 indicates homology		 QNITETSFVLR + 2 Deamidated (NQ)
2012 453.5818 1357.7236 1357.7201 2.55 1 19 0.82 +1Score > 40 indicates identity
Score > 30 indicates homology		 QTALSIEQQLAR + Deamidated (NQ)
1311 514.8256 1027.6366 1027.6277 8.68 1 19 0.78 +1Score > 35 indicates identity
Score > 30 indicates homology		 LVGLLEGSLK
1679 601.3412 1200.6678 1200.6601 6.42 1 19 0.73 +1Score > 40 indicates identity
Score > 30 indicates homology		 DLVQQKDILK + 2 Deamidated (NQ)
1207 330.5144 988.5214 988.5077 13.9 1 19 0.9 +1Score > 42 indicates identity
Score > 31 indicates homology		 LTDLQEAAK + Deamidated (NQ)
1078 470.7920 939.5694 939.5753 -6.23 0 19 0.77 +1Score > 35 indicates identity
Score > 30 indicates homology		 QPVLLITR + Deamidated (NQ)
3218 670.7067 2009.0983 2009.1381 -19.8 1 19 0.14 +1Score > 36 indicates identity
Score > 23 indicates homology		 TSLGTINHSLLSLAALRSR
3803 794.1021 2379.2845 2379.2885 -1.71 0 19 0.17 +1Score > 35 indicates identity
Score > 23 indicates homology		 FGIGADGVLFLAKPLHTSLHMR
1428 362.8674 1085.5804 1085.5968 -15.2 0 19 0.27 +1Score > 41 indicates identity
Score > 26 indicates homology		 QVVTLVQGIE + Deamidated (NQ)
616 375.7256 749.4366 749.4436 -9.24 0 19 0.68 +1Score > 36 indicates identity
Score > 29 indicates homology		 ITFTLR
1062 311.1797 930.5173 930.5134 4.16 1 19 0.87 +1Score > 42 indicates identity
Score > 31 indicates homology		 TANLLKNR + 2 Deamidated (NQ)
1484 560.7956 1119.5766 1119.5924 -14.1 1 19 0.49 +1Score > 42 indicates identity
Score > 28 indicates homology		 GLQLFSVARE + Deamidated (NQ)
1507 564.8219 1127.6292 1127.6299 -0.54 1 19 0.95 +1Score > 40 indicates identity
Score > 31 indicates homology		 NALLENVTVR
2220 730.8435 1459.6724 1459.6678 3.18 1 19 0.65 +1Score > 40 indicates identity
Score > 29 indicates homology		 QVQAELQQLGGSAE + 3 Deamidated (NQ)
3573 1104.5914 2207.1682 2207.1474 9.45 1 19 0.06 +1Score > 37 indicates identity
Score > 19 indicates homology		 DLLGTLEVPADAYYGIQTLR
1229 333.1645 996.4717 996.4764 -4.72 0 18 0.23 +1Score > 40 indicates identity
Score > 25 indicates homology		 ISNVSFDSK + Deamidated (NQ)
708 395.2363 788.4580 788.4432 18.8 0 18 0.73 +1Score > 39 indicates identity
Score > 30 indicates homology		 LFGPDLK
1931 331.2000 1320.7709 1320.7765 -4.26 1 18 0.96 +1Score > 32 indicates identity
Score > 31 indicates homology		 KPTSHILVEVAK
845 426.7145 851.4144 851.4025 14.1 1 18 0.15 +1Score > 41 indicates identity
Score > 23 indicates homology		 TAAQFEGK + Deamidated (NQ)
1370 529.7931 1057.5716 1057.5702 1.35 1 18 0.85 +1Score > 42 indicates identity
Score > 30 indicates homology		 LAVMPQSRR + Deamidated (NQ)
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