MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1402 536.7877 1071.5608 1071.5560 4.51 1 22 0.85 +1Score > 42 indicates identity
Score > 34 indicates homology		 VQQALDELR + Deamidated (NQ)
652 382.7337 763.4528 763.4480 6.41 0 22 0.78 +1Score > 35 indicates identity
Score > 34 indicates homology		 IYNIIK + Deamidated (NQ)
2004 452.5634 1354.6684 1354.6742 -4.29 1 22 0.097 +1Score > 40 indicates identity
Score > 24 indicates homology		 GAAVWGPRAQADR + Deamidated (NQ)
1174 486.7666 971.5186 971.5148 3.93 0 22 0.8 +1Score > 42 indicates identity
Score > 34 indicates homology		 SAGLQVQNR
2741 851.9210 1701.8274 1701.8210 3.82 0 22 0.61 +1Score > 40 indicates identity
Score > 32 indicates homology		 LTNTNINQLDQWIE + Deamidated (NQ)
995 457.7925 913.5704 913.5596 11.8 0 22 0.26 +1Score > 36 indicates identity
Score > 29 indicates homology		 ALLSQLLR + Deamidated (NQ)
974 452.2473 902.4800 902.4709 10.2 1 22 0.79 +1Score > 42 indicates identity
Score > 34 indicates homology		 LTNADLEK
2985 615.3308 1842.9706 1842.9799 -5.07 0 22 0.53 +1Score > 39 indicates identity
Score > 32 indicates homology		 SVTINALSGVSGAGAKPSAR + Deamidated (NQ)
2826 582.6248 1744.8526 1744.8843 -18.2 1 22 0.1 +1Score > 40 indicates identity
Score > 25 indicates homology		 ISQNPTAVLTETVSQR + 2 Deamidated (NQ)
2539 538.2914 1611.8524 1611.8580 -3.49 1 22 0.28 +1Score > 39 indicates identity
Score > 29 indicates homology		 LSARPGNEVNLLDSK
2437 783.9054 1565.7962 1565.8236 -17.4 1 22 0.33 +1Score > 40 indicates identity
Score > 30 indicates homology		 TAPKTTALSVAGMFR + Oxidation (M)
2432 781.9117 1561.8088 1561.7923 10.6 0 22 0.7 +1Score > 40 indicates identity
Score > 33 indicates homology		 MIVFVNQRPVDSR + 2 Deamidated (NQ)
306 602.3531 601.3458 601.3435 3.85 0 22 0.6 +1Score > 45 indicates identity
Score > 32 indicates homology		 LSAQIA
2482 793.4343 1584.8540 1584.8512 1.82 0 22 0.17 +1Score > 39 indicates identity
Score > 27 indicates homology		 TVILWDVAQGQVVR + 2 Deamidated (NQ)
734 400.7495 799.4844 799.4916 -8.90 0 22 0.2 +1Score > 40 indicates identity
Score > 27 indicates homology		 LVSALAAR
331 308.6753 615.3360 615.3452 -14.9 1 22 0.68 +1Score > 44 indicates identity
Score > 33 indicates homology		 AGNKAR
1403 358.5166 1072.5280 1072.5335 -5.16 1 22 0.13 +1Score > 41 indicates identity
Score > 25 indicates homology		 MNGPGALELR + Oxidation (M)
2764 571.3336 1710.9790 1710.9767 1.30 1 22 0.19 +1Score > 34 indicates identity
Score > 27 indicates homology		 QQPITTTLELVDIIK
1704 304.6738 1214.6661 1214.6619 3.48 1 22 0.38 +1Score > 40 indicates identity
Score > 30 indicates homology		 ALIQSAIGSAER
440 673.3995 672.3922 672.3918 0.56 1 22 0.74 +1Score > 43 indicates identity
Score > 33 indicates homology		 KQGLAR + Deamidated (NQ)
1167 486.7552 971.4958 971.5036 -7.96 1 22 0.76 +1Score > 41 indicates identity
Score > 33 indicates homology		 QLIQQQRG + 2 Deamidated (NQ)
1299 512.2616 1022.5086 1022.5284 -19.3 0 22 0.18 +1Score > 42 indicates identity
Score > 27 indicates homology		 IADTLDTFK
632 380.2361 758.4576 758.4538 5.10 0 22 0.97 +1Score > 40 indicates identity
Score > 34 indicates homology		 IISDALK
894 435.2472 868.4798 868.4807 -0.96 0 22 0.13 +1Score > 37 indicates identity
Score > 25 indicates homology		 ILTYGFR
3005 619.6663 1855.9771 1855.9891 -6.48 0 22 0.12 +1Score > 38 indicates identity
Score > 25 indicates homology		 VLTSDSNIVPVDISGIAR + Deamidated (NQ)
580 365.2260 728.4374 728.4432 -7.93 1 22 0.96 +1Score > 41 indicates identity
Score > 34 indicates homology		 ALEGVIK
1084 471.7517 941.4888 941.5069 -19.2 1 22 0.63 +1Score > 40 indicates identity
Score > 32 indicates homology		 LLIENDPK + Deamidated (NQ)
1199 328.8551 983.5435 983.5512 -7.83 1 21 0.28 +1Score > 37 indicates identity
Score > 28 indicates homology		 QAKLNRPR + 2 Deamidated (NQ)
2297 501.9216 1502.7430 1502.7148 18.8 0 21 0.19 +1Score > 41 indicates identity
Score > 27 indicates homology		 MTQVGQTHTSGSIR + Deamidated (NQ)
1287 339.4994 1015.4764 1015.4822 -5.72 1 21 0.18 +1Score > 40 indicates identity
Score > 27 indicates homology		 QPLNTGEQK + 2 Deamidated (NQ)
2395 516.6040 1546.7902 1546.8025 -7.95 1 21 0.19 +1Score > 41 indicates identity
Score > 27 indicates homology		 TGIDLKGDIMALQR + Deamidated (NQ); Oxidation (M)
1129 319.8390 956.4952 956.5113 -16.9 0 21 0.36 +1Score > 39 indicates identity
Score > 29 indicates homology		 LCLPQGLR + Deamidated (NQ)
2906 599.3317 1794.9733 1794.9727 0.33 0 21 0.19 +1Score > 37 indicates identity
Score > 27 indicates homology		 IQNIVASANLGKPLNLE + 2 Deamidated (NQ)
544 358.2460 714.4774 714.4640 18.9 0 21 0.36 +1Score > 36 indicates identity
Score > 29 indicates homology		 GIVVISK
701 392.7572 783.4998 783.4967 4.05 0 21 0.83 +1Score > 36 indicates identity
Score > 33 indicates homology		 VAIVQVR
831 848.3923 847.3850 847.3937 -10.2 1 21 0.73 +1Score > 41 indicates identity
Score > 33 indicates homology		 DDTWRR
345 311.6595 621.3044 621.2970 12.1 0 21 0.73 +1Score > 40 indicates identity
Score > 33 indicates homology		 NATSTK + Deamidated (NQ)
669 386.7257 771.4368 771.4239 16.8 1 21 0.83 +1Score > 43 indicates identity
Score > 33 indicates homology		 IVEAANR
793 416.2632 830.5118 830.4974 17.4 1 21 0.93 +1Score > 35 indicates identity
Score > 34 indicates homology		 KLTGAVSR
332 308.6754 615.3362 615.3340 3.65 0 21 0.58 +1Score > 44 indicates identity
Score > 31 indicates homology		 AAGGAAAK
1706 304.6744 1214.6685 1214.6619 5.46 1 21 0.67 +1Score > 40 indicates identity
Score > 32 indicates homology		 ALIQSAIGSAER
1131 479.2556 956.4966 956.4927 4.12 1 21 0.32 +1Score > 40 indicates identity
Score > 29 indicates homology		 TPVAEDVAR
853 854.4765 853.4692 853.4657 4.10 0 21 0.27 +1Score > 37 indicates identity
Score > 28 indicates homology		 APAAAPAASK
2781 574.2916 1719.8530 1719.8654 -7.24 0 21 0.2 +1Score > 41 indicates identity
Score > 27 indicates homology		 LMHFQTIPYSPTLR + Deamidated (NQ); Oxidation (M)
1910 439.2503 1314.7291 1314.7143 11.2 1 21 0.34 +1Score > 40 indicates identity
Score > 29 indicates homology		 IVLNIGSGAESVR + Deamidated (NQ)
1417 361.8498 1082.5276 1082.5430 -14.3 0 21 0.71 +1Score > 40 indicates identity
Score > 32 indicates homology		 NPHLIMNVK + 2 Deamidated (NQ); Oxidation (M)
1594 583.7858 1165.5570 1165.5476 8.14 0 21 0.63 +1Score > 41 indicates identity
Score > 32 indicates homology		 NAPIHSQQNR + 2 Deamidated (NQ)
336 308.6879 615.3612 615.3704 -14.9 1 21 0.52 +1Score > 45 indicates identity
Score > 31 indicates homology		 AGGGKVK
1155 483.7498 965.4850 965.5038 -19.4 1 21 0.86 +1Score > 42 indicates identity
Score > 33 indicates homology		 KGAMTALMK + Oxidation (M)
836 425.2472 848.4798 848.4868 -8.19 1 21 0.67 +1Score > 40 indicates identity
Score > 32 indicates homology		 LYRQIR + Deamidated (NQ)
2237 736.3753 1470.7360 1470.7566 -13.9 0 21 0.17 +1Score > 41 indicates identity
Score > 26 indicates homology		 NNDILLLQGAGTIE + Deamidated (NQ)
952 897.5338 896.5265 896.5443 -19.9 1 21 0.63 +1Score > 36 indicates identity
Score > 32 indicates homology		 KPLVVEGR
200 474.2704 473.2631 473.2598 7.06 0 21 0.28 +1Score > 47 indicates identity
Score > 28 indicates homology		 AGAAGK
1099 316.4922 946.4548 946.4720 -18.2 1 21 0.74 +1Score > 42 indicates identity
Score > 32 indicates homology		 GENIGTTVR + Deamidated (NQ)
1656 596.7985 1191.5824 1191.5983 -13.3 1 21 0.86 +1Score > 41 indicates identity
Score > 33 indicates homology		 GITLASASSQEK + Deamidated (NQ)
1371 529.7997 1057.5848 1057.5768 7.66 1 21 0.8 +1Score > 41 indicates identity
Score > 33 indicates homology		 QTQAILGAEK
2601 545.6293 1633.8661 1633.8974 -19.2 1 21 0.79 +1Score > 39 indicates identity
Score > 33 indicates homology		 YLQATPMVIGIRTR + Oxidation (M)
2806 866.9469 1731.8792 1731.8890 -5.65 0 21 0.4 +1Score > 40 indicates identity
Score > 29 indicates homology		 LVQAVIAGGSSSLINSSE + Deamidated (NQ)
460 340.6807 679.3468 679.3442 3.91 0 21 0.9 +1Score > 40 indicates identity
Score > 33 indicates homology		 FPFNR
748 403.7254 805.4362 805.4334 3.56 0 21 0.13 +1Score > 41 indicates identity
Score > 25 indicates homology		 IFNNIGK + Deamidated (NQ)
1863 645.3397 1288.6648 1288.6809 -12.5 1 21 0.86 +1Score > 42 indicates identity
Score > 33 indicates homology		 QTILQMGRVDK + Deamidated (NQ)
936 444.7578 887.5010 887.5076 -7.38 1 21 0.28 +1Score > 41 indicates identity
Score > 28 indicates homology		 IASNVQKK + Deamidated (NQ)
1481 373.8549 1118.5429 1118.5390 3.46 0 21 0.78 +1Score > 42 indicates identity
Score > 32 indicates homology		 QAGVSCQQLK + Deamidated (NQ)
824 422.7133 843.4120 843.4086 4.07 1 21 0.89 +1Score > 39 indicates identity
Score > 33 indicates homology		 IQSENPR + Deamidated (NQ)
1252 503.2971 1004.5796 1004.5654 14.1 0 21 0.91 +1Score > 37 indicates identity
Score > 33 indicates homology		 AIDAYIALR
1455 551.3005 1100.5864 1100.5826 3.49 0 21 0.82 +1Score > 42 indicates identity
Score > 32 indicates homology		 GIVTGDLGVDR
1008 459.2461 916.4776 916.4654 13.4 0 21 0.19 +1Score > 43 indicates identity
Score > 26 indicates homology		 DAIVNAWK + Deamidated (NQ)
1233 333.1960 996.5662 996.5465 19.8 1 21 0.66 +1Score > 38 indicates identity
Score > 31 indicates homology		 QARNGVPVR + Deamidated (NQ)
446 337.2171 672.4196 672.4170 3.90 0 21 0.78 +1Score > 40 indicates identity
Score > 32 indicates homology		 SGLIVGK
1951 444.5938 1330.7596 1330.7609 -0.98 1 21 0.22 +1Score > 36 indicates identity
Score > 26 indicates homology		 FANVLKDLGVQK
2041 686.3704 1370.7262 1370.7405 -10.4 1 21 0.43 +1Score > 40 indicates identity
Score > 29 indicates homology		 VLSQQAAAVVKQE + Deamidated (NQ)
3133 488.7507 1950.9737 1950.9660 3.94 1 21 0.18 +1Score > 40 indicates identity
Score > 26 indicates homology		 SNRHIYAQVIDDVHQR + Deamidated (NQ)
1244 501.7843 1001.5540 1001.5505 3.51 0 21 0.6 +1Score > 41 indicates identity
Score > 31 indicates homology		 ANTIDISLR
2489 397.7165 1586.8369 1586.8126 15.3 1 21 0.22 +1Score > 40 indicates identity
Score > 27 indicates homology		 RMLNWLLNPITSE + Deamidated (NQ)
2394 774.3998 1546.7850 1546.8103 -16.3 1 21 0.36 +1Score > 41 indicates identity
Score > 29 indicates homology		 EAAAPGAAPAAAAAAPAAK
2510 533.6335 1597.8787 1597.9039 -15.8 1 21 0.066 +1Score > 38 indicates identity
Score > 21 indicates homology		 LATEAAAAQIVTVAIR + Deamidated (NQ)
2295 501.9188 1502.7346 1502.7148 13.2 0 20 0.48 +1Score > 41 indicates identity
Score > 30 indicates homology		 MTQVGQTHTSGSIR + Deamidated (NQ)
1611 587.2862 1172.5578 1172.5350 19.5 0 20 0.18 +1Score > 40 indicates identity
Score > 26 indicates homology		 LGYNDTQFSK + Deamidated (NQ)
370 316.6921 631.3696 631.3653 6.85 0 20 0.93 +1Score > 41 indicates identity
Score > 33 indicates homology		 VASVTR
1643 593.3487 1184.6828 1184.6765 5.40 1 20 0.57 +1Score > 38 indicates identity
Score > 31 indicates homology		 VIQVNNQKLK + 2 Deamidated (NQ)
1058 465.7864 929.5582 929.5545 3.98 0 20 0.86 +1Score > 38 indicates identity
Score > 32 indicates homology		 LTNLIASAK
1950 444.5921 1330.7545 1330.7609 -4.82 1 20 0.2 +1Score > 37 indicates identity
Score > 26 indicates homology		 FANVLKDLGVQK
1274 506.3136 1010.6126 1010.6012 11.4 0 20 0.48 +1Score > 33 indicates identity
Score > 30 indicates homology		 INALQLIPK + 2 Deamidated (NQ)
2219 730.8426 1459.6706 1459.6678 1.95 1 20 0.22 +1Score > 40 indicates identity
Score > 26 indicates homology		 QVQAELQQLGGSAE + 3 Deamidated (NQ)
2544 806.9435 1611.8724 1611.8733 -0.50 1 20 0.51 +1Score > 38 indicates identity
Score > 30 indicates homology		 INAIINDGKYHVVR + Deamidated (NQ)
1544 380.8908 1139.6506 1139.6550 -3.88 0 20 0.69 +1Score > 37 indicates identity
Score > 31 indicates homology		 AIASAVNPVAVK + Deamidated (NQ)
1516 566.2884 1130.5622 1130.5642 -1.69 0 20 0.61 +1Score > 42 indicates identity
Score > 31 indicates homology		 VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
2848 882.4977 1762.9808 1763.0080 -15.4 1 20 0.17 +1Score > 35 indicates identity
Score > 25 indicates homology		 IIENLKPLLPAGIQDK + 2 Deamidated (NQ)
1807 632.8148 1263.6150 1263.6315 -13.0 1 20 0.87 +1Score > 41 indicates identity
Score > 32 indicates homology		 CILQKLMQGR + 2 Deamidated (NQ); Oxidation (M)
941 447.7388 893.4630 893.4528 11.4 1 20 0.84 +1Score > 41 indicates identity
Score > 32 indicates homology		 TGVEMTLK + Oxidation (M)
1189 326.8755 977.6047 977.5910 14.0 0 20 0.6 +1Score > 31 indicates identity
Score > 31 indicates homology		 LPGPLPVGTK
1948 665.3489 1328.6832 1328.6936 -7.78 1 20 0.19 +1Score > 41 indicates identity
Score > 26 indicates homology		 QVVVNLLGSRGGE + 2 Deamidated (NQ)
1746 615.3645 1228.7144 1228.7027 9.59 1 20 0.33 +1Score > 37 indicates identity
Score > 28 indicates homology		 QLSEGLLALSAK
3404 530.2810 2117.0949 2117.0905 2.05 0 20 0.12 +1Score > 39 indicates identity
Score > 24 indicates homology		 QYIAQHFVAISTNANDVVK
887 433.7179 865.4212 865.4294 -9.36 1 20 0.23 +1Score > 41 indicates identity
Score > 26 indicates homology		 LAEAGYSR
1200 492.8129 983.6112 983.6015 9.91 0 20 0.26 +1Score > 35 indicates identity
Score > 27 indicates homology		 QLAILADLK
1415 360.8546 1079.5420 1079.5355 6.02 1 20 0.21 +1Score > 41 indicates identity
Score > 26 indicates homology		 KQQVAMMVK + 2 Deamidated (NQ); Oxidation (M)
546 716.3978 715.3905 715.3977 -10.0 0 20 0.85 +1Score > 43 indicates identity
Score > 32 indicates homology		 QVSVAGR
1878 648.8702 1295.7258 1295.7085 13.4 1 20 0.81 +1Score > 37 indicates identity
Score > 32 indicates homology		 INNLNHSILKK + 3 Deamidated (NQ)
2885 893.9840 1785.9534 1785.9585 -2.82 1 20 0.83 +1Score > 39 indicates identity
Score > 32 indicates homology		 RIQGGAADTVLLGATGTGK + Deamidated (NQ)
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