MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4571 874.7481 3494.9633
4572 1168.2650 3501.7732
4573 706.1740 3525.8336
4574 706.3712 3526.8196
4575 1178.6390 3532.8952
4576 884.4840 3533.9069
4579 887.2333 3544.9041
4580 887.7354 3546.9125
4582 891.4599 3561.8105
4585 715.0031 3569.9791
4586 715.0038 3569.9826
4587 893.5044 3569.9885
4588 1191.6421 3571.9045
4589 894.4968 3573.9581
4592 718.9878 3589.9026
4593 903.4836 3609.9053
4596 906.4999 3621.9705
4597 907.2172 3624.8397
4602 914.7278 3654.8821
4603 917.4909 3665.9345
4604 919.0135 3672.0249
4605 921.0042 3679.9877
4606 921.2576 3681.0013
4607 737.3827 3681.8771
4608 921.5098 3682.0101
4609 921.5116 3682.0173
4611 738.3529 3686.7281
4612 925.4512 3697.7757
4613 741.6191 3703.0591
4617 934.4791 3733.8873
4619 937.5172 3746.0397
4620 938.5273 3750.0801
4622 947.5213 3786.0561
4623 951.5031 3801.9833
4624 951.5211 3802.0553
4625 762.4284 3807.1056
4626 957.5098 3826.0101
4627 957.5204 3826.0525
4632 777.2057 3880.9921
4633 972.0156 3884.0333
4638 654.1441 3918.8209
4640 788.4119 3937.0231
4642 989.5439 3954.1465
4644 1002.2622 4005.0197
4645 1002.2633 4005.0241
4646 1002.2862 4005.1157
4647 1002.2888 4005.1261
4648 1002.2890 4005.1269
4649 802.0341 4005.1341
4650 803.2003 4010.9651
4651 1003.9965 4011.9569
4653 1003.9981 4011.9633
4654 803.4003 4011.9651
4655 804.2412 4016.1696
4656 1007.5082 4026.0037
4657 1008.0649 4028.2305
4658 809.3992 4041.9596
4660 1013.0385 4048.1249
4662 1016.5168 4062.0381
4663 1016.5193 4062.0481
4664 1016.5437 4062.1457
4665 1016.5443 4062.1481
4666 1016.5477 4062.1617
4667 1016.7817 4063.0977
4668 1016.7842 4063.1077
4669 1016.7856 4063.1133
4670 1017.9990 4067.9669
4671 1018.2521 4068.9793
4673 814.8065 4068.9961
4674 1020.5315 4078.0969
4675 1020.5441 4078.1473
4676 1022.5582 4086.2037
4677 1022.5715 4086.2569
4678 1030.5518 4118.1781
4679 824.6480 4118.2036
4681 1032.5078 4126.0021
4682 826.2087 4126.0071
4686 835.8521 4174.2241
4687 1045.5796 4178.2893
4688 1051.5415 4202.1369
4689 1051.5504 4202.1725
4691 1052.5510 4206.1749
4692 1053.5262 4210.0757
4694 1064.0204 4252.0525
4696 1079.8151 4315.2313
4699 870.0342 4345.1346
4702 1117.5892 4466.3277
4704 902.4907 4507.4171
4705 904.8833 4519.3801
4708 1138.3544 4549.3885
4709 910.8945 4549.4361
4711 1138.6082 4550.4037
4712 912.1248 4555.5876
4714 1144.6024 4574.3805
4715 1145.0864 4576.3165
4716 1145.6394 4578.5285
4717 928.6811 4638.3691
4718 1164.8191 4655.2473
4719 940.0824 4695.3756
4720 1175.0953 4696.3521
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