MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4335 757.9299 3027.6905
4337 1010.5646 3028.6720
4340 607.5144 3032.5356
4343 1013.5175 3037.5307
4344 1013.5217 3037.5433
4346 760.6555 3038.5929
4349 762.1755 3044.6729
4350 762.4142 3045.6277
4360 1023.2236 3066.6490
4362 767.8573 3067.4001
4364 1024.2368 3069.6886
4365 1024.5756 3070.7050
4368 770.8895 3079.5289
4370 771.1415 3080.5369
4371 1028.2174 3081.6304
4375 1030.5122 3088.5148
4379 773.9236 3091.6653
4386 1035.5687 3103.6843
4397 1045.5262 3133.5568
4407 787.6754 3146.6725
4409 790.4295 3157.6889
4417 792.4254 3165.6725
4419 1057.9569 3170.8489
4420 1057.9724 3170.8954
4423 795.4094 3177.6085
4424 1060.9124 3179.7154
4427 1061.5236 3181.5490
4431 1061.5288 3181.5646
4433 1062.8981 3185.6725
4435 1064.6155 3190.8247
4436 1064.8990 3191.6752
4442 800.4091 3197.6073
4444 641.3645 3201.7861
4446 801.4611 3201.8153
4447 801.7065 3202.7969
4450 802.4239 3205.6665
4451 802.4242 3205.6677
4454 1070.5613 3208.6621
4457 803.3964 3209.5565
4461 803.4169 3209.6385
4466 1075.5275 3223.5607
4467 1077.5511 3229.6315
4469 1078.1851 3231.5335
4471 1078.5886 3232.7440
4474 1079.5764 3235.7074
4475 1079.9006 3236.6800
4476 810.1949 3236.7505
4477 1080.5258 3238.5556
4480 813.4592 3249.8077
4481 813.7081 3250.8033
4482 1084.6124 3250.8154
4483 1085.2640 3252.7702
4486 815.4404 3257.7325
4488 1087.5583 3259.6531
4491 817.4070 3265.5989
4492 817.4600 3265.8109
4493 819.4369 3273.7185
4494 819.6588 3274.6061
4495 819.6682 3274.6437
4496 1092.5824 3274.7254
4498 1094.5551 3280.6435
4499 657.5612 3282.7696
4500 821.9535 3283.7849
4501 822.4127 3285.6217
4502 1097.6093 3289.8061
4503 1099.7849 3296.3329
4504 661.1799 3300.8631
4505 826.4210 3301.6549
4506 826.6651 3302.6313
4508 662.1720 3305.8236
4509 827.4667 3305.8377
4511 827.7109 3306.8145
4512 1103.2789 3306.8149
4515 827.7129 3306.8225
4517 827.9623 3307.8201
4518 827.9671 3307.8393
4519 664.3603 3316.7651
4523 666.1501 3325.7141
4524 832.4487 3325.7657
4528 1112.5883 3334.7431
4529 1114.6057 3340.7953
4530 669.3649 3341.7881
4535 838.2263 3348.8761
4536 1118.3162 3351.9268
4537 840.4716 3357.8573
4538 1120.9490 3359.8252
4542 841.9634 3363.8245
4543 841.9656 3363.8333
4545 673.7750 3363.8386
4546 1122.6188 3364.8346
4548 844.4374 3373.7205
4549 678.3528 3386.7276
4550 1130.6007 3388.7803
4554 680.9673 3399.8001
4555 681.9841 3404.8841
4557 852.4875 3405.9209
4558 683.5939 3412.9331
4559 854.2420 3412.9389
4561 854.4615 3413.8169
4569 1149.5787 3445.7143
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