MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3989 857.1494 2568.4264
3992 645.3337 2577.3057
3993 645.3343 2577.3081
3995 860.7949 2579.3629
3997 861.1447 2580.4123
3998 861.1503 2580.4291
3999 861.4506 2581.3300
4000 861.4534 2581.3384
4001 861.4689 2581.3849
4014 870.4700 2608.3882
4023 877.4676 2629.3810
4029 879.4172 2635.2298
4031 879.4178 2635.2316
4034 880.7998 2639.3776
4037 882.7991 2645.3755
4041 530.6515 2648.2211
4045 887.1818 2658.5236
4048 888.9979 2663.9719
4057 672.3177 2685.2417
4072 898.4528 2692.3366
4090 679.3621 2713.4193
4091 908.0842 2721.2308
4092 908.0847 2721.2323
4097 910.8594 2729.5564
4098 910.8615 2729.5627
4099 683.3989 2729.5665
4101 683.5790 2730.2869
4104 911.7935 2732.3587
4106 912.1105 2733.3097
4111 686.3217 2741.2577
4112 686.6464 2742.5565
4119 917.8497 2750.5273
4121 917.8507 2750.5303
4122 688.6401 2750.5313
4123 918.1864 2751.5374
4127 690.3763 2757.4761
4129 690.3868 2757.5181
4131 690.8616 2759.4173
4136 922.8974 2765.6704
4144 926.1859 2775.5359
4148 927.4998 2779.4776
4151 557.0909 2780.4181
4160 698.1276 2788.4813
4161 930.5116 2788.5130
4165 931.5192 2791.5358
4168 932.1778 2793.5116
4172 935.5125 2803.5157
4183 937.1872 2808.5398
4185 939.5196 2815.5370
4189 941.1418 2820.4036
4193 941.8486 2822.5240
4196 943.1614 2826.4624
4203 948.5567 2842.6483
4205 712.9033 2847.5841
4206 950.4967 2848.4683
4211 955.8533 2864.5381
4212 955.8749 2864.6029
4214 956.1617 2865.4633
4215 717.3935 2865.5449
4217 718.3832 2869.5037
4219 718.3855 2869.5129
4220 718.8934 2871.5445
4221 718.8937 2871.5457
4224 958.8836 2873.6290
4233 721.1529 2880.5825
4237 963.2140 2886.6202
4238 963.4972 2887.4698
4246 967.8450 2900.5132
4248 726.1600 2900.6109
4249 726.4160 2901.6349
4251 727.8674 2907.4405
4252 970.4958 2908.4656
4253 728.1304 2908.4925
4255 728.4059 2909.5945
4257 971.5096 2911.5070
4269 733.1489 2928.5665
4275 978.8467 2933.5183
4278 738.4020 2949.5789
4279 984.2032 2949.5878
4280 738.6271 2950.4793
4281 984.5292 2950.5658
4283 985.5263 2953.5571
4291 741.8731 2963.4633
4292 742.3865 2965.5169
4295 990.5289 2968.5649
4296 990.8519 2969.5339
4297 991.2440 2970.7102
4299 995.5225 2983.5457
4305 997.5294 2989.5664
4307 748.4074 2989.6005
4308 748.8925 2991.5409
4314 998.5024 2992.4854
4316 998.5056 2992.4950
4317 998.5064 2992.4974
4325 754.9303 3015.6921
4327 1006.5767 3016.7083
4328 1007.7912 3020.3518
4329 1007.7921 3020.3545
4332 1008.5181 3022.5325
4334 757.6456 3026.5533
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